Production of Cellulolytic Enzymes by Aspergillus phoenicis in Grape Waste using Response Surface Methodology

• Published in: Applied biochemistry and biotechnology Vol. 152; no. 2; pp. 295 - 305
• Main Authors: Dedavid e Silva, Lucas André, Cortez Lopes, Fernanda, Terra Silveira, Silvana, Brandelli, Adriano
• Format: Journal Article
• Language: English
• Published: New York New York : Humana Press Inc 01.02.2009; Humana Press Inc; Springer; Springer Nature B.V
• Subjects: agricultural wastes; Aspergillus; Aspergillus - metabolism; Aspergillus phoenicis; beta-glucosidase; beta-Glucosidase - biosynthesis; Biochemistry; Biological and medical sciences; Biotechnology; Cellulase - biosynthesis; cellulases; Cellulases - biosynthesis; cellulose; Chemistry; Chemistry and Materials Science; Enzymes; Fermentation; Fruits; Fundamental and applied biological sciences. Psychology; Fungi; grapes; Industrial wastes; Peptones; response surface methodology; Vitaceae; Vitis - microbiology; Waste Products; waste utilization; winemaking; Wines; Agro-industrial waste; β-Glucosidases; Factorial design; Cellulase; Enzyme; Aspergillus phoenicis; Fungi; Glycosylases; Design; Statistical method; Agro-industrial waste . Factorial design . Cellulase; β-Glucosidase; Cellulolytic enzyme; Glycosidases; Grape; Production; Hydrolases; Response surface; Fungi Imperfecti; Wastes
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-008-8190-7

The production of cellulolytic enzymes by the fungus Aspergillus phoenicis was investigated. Grape waste from the winemaking industry was chosen as the growth substrate among several agro-industrial byproducts. A 2 x 2 central composite design was performed, utilizing the amount of grape waste and peptone as independent variables. The fungus was cultivated in submerged fermentation at 30 °C and 120 rpm for 120 h, and the activities of total cellulases, endoglucanases, and β-glucosidases were measured. Total cellulases were positively influenced by the linear increase of peptone concentration and decrease at axial concentrations of grape waste and peptone. Maximum activity of endoglucanase was observed by a linear increase of both grape waste and peptone concentrations. Concentrations of grape waste between 5 and 15 g/L had a positive effect on the production of β-glucosidase; peptone had no significant effects. The optimum production of the three cellulolytic activities was observed at values near the central point. A. phoenicis has the potential for the production of cellulases utilizing grape waste as the growth substrate.