Comparative effects of insulin and insulin-like growth factors on DNA synthesis and differentiation of porcine granulosa cells

• Published in: Biochemical and biophysical research communications Vol. 124; no. 2; pp. 484 - 490
• Main Authors: Baranao, J.L.S., Hammond, J.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.10.1984
• Subjects: Animals; Cell Differentiation - drug effects; Cells, Cultured; differentiation; DNA biosynthesis; DNA Replication - drug effects; Female; granulosa cells; Granulosa Cells - drug effects; Granulosa Cells - metabolism; IGF-I; IGF-II; insulin; Insulin - metabolism; Insulin - pharmacology; Kinetics; Peptides - metabolism; Peptides - pharmacology; pigs; Progesterone - metabolism; Receptors, Cell Surface - drug effects; Receptors, Cell Surface - metabolism; Receptors, Somatomedin; Somatomedins - metabolism; Somatomedins - pharmacology; Swine; Multiplication stimulating activity; Insulin-like growth factors I and II
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(84)91579-1

The effects of insulin-like growth factors (IGF-I and II) were compared with those of insulin in porcine granulosa cells in vitro with regard to stimulation of thymidine incorporation, facilitation of FSH-induced progesterone secretion, and displacement of [ 125I] IGF-I from specific receptors. In all three assays, the order of potency was IGF-I > IGF-II > insulin. However, the difference in potency was marginally significant in the mitogenic assay. These data are consistent with the presence of specific IGF-I receptors on these cells and involvement of these binding sites in regulation of granulosa cell replication and differentiation.