IL-17RA aptamer-mediated repression of IL-6 inhibits synovium inflammation in a murine model of osteoarthritis

• Published in: Osteoarthritis and cartilage Vol. 19; no. 6; pp. 711 - 718
• Main Authors: Chen, L, Li, D.Q, Zhong, J, Wu, X.L, Chen, Q, Peng, H, Liu, S.Q
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2011
• Subjects: Animals; Aptamer; Celecoxib; Cell-SELEX; Cyclooxygenase Inhibitors - pharmacology; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; IL-17RA; Inflammation - metabolism; Inflammation - pathology; Interleukin-17 - metabolism; Interleukin-6 - metabolism; Mice; Osteoarthritis; Osteoarthritis, Knee - drug therapy; Osteoarthritis, Knee - metabolism; Osteoarthritis, Knee - pathology; Polymerase Chain Reaction; Pyrazoles - pharmacology; Rheumatology; RNA, Messenger - metabolism; Sulfonamides - pharmacology; Synovial Membrane - drug effects; Synovial Membrane - pathology; Synovium inflammation; Aptamer; Synovium inflammation; Celecoxib; Osteoarthritis; Cell-SELEX; IL-17RA

Summary Objective Generate DNA aptamers to inhibit IL-17RA-mediated synovial inflammation in an experimental mouse model of osteoarthritis (OA). Methods A novel cell-SELEX method was applied to obtain DNA aptamers specific for IL-17RA. A single-stranded (ss) DNA library with four probes was synthesi...