Bacteriophage-derived enzyme that depolymerizes the alginic acid capsule associated with cystic fibrosis isolates of Pseudomonas aeruginosa

• Published in: Journal of applied microbiology Vol. 108; no. 2; pp. 695 - 702
• Main Authors: Glonti, T, Chanishvili, N, Taylor, P.W
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: alginate lyase; alginates; Alginates - metabolism; alginic acid; Bacteria; Bacterial Capsules - metabolism; bacteriophage; Bacteriophages - enzymology; Biofilms; Biological and medical sciences; Cystic Fibrosis - microbiology; exopolysaccharide depolymerase; Fundamental and applied biological sciences. Psychology; Glucuronic Acid - metabolism; Hexuronic Acids - metabolism; Microbiology; Podoviridae; Pseudomonas aeruginosa; Pseudomonas aeruginosa - virology; Pseudomonas Infections - microbiology; Viral Plaque Assay; Pseudomonadales; Polysaccharide-lyases; alginate lyase; Poly(β-D-mannuronate) lyase; Enzyme; Applied microbiology; Cystic fibrosis; Lyases; Capsule; Extracellular; bacteriophage; Virus; Bacteria; Pseudomonadaceae; Isolate; Pseudomonas aeruginosa; exopolysaccharide depolymerase; alginic acid; Carbon-oxygen lyases; Polysaccharide; Phage

To identify enzymes associated with bacteriophages infecting cystic fibrosis (CF) strains of Pseudomonas aeruginosa that are able to degrade extracellular alginic acids elaborated by the host bacterium. Plaques produced by 21 Ps. aeruginosa-specific phages were screened for the presence of haloes, a...