Reversible phosphocholination of Rab proteins by Legionella pneumophila effector proteins

• Published in: The EMBO journal Vol. 31; no. 7; pp. 1774 - 1784
• Main Authors: Goody, Philip R, Heller, Katharina, Oesterlin, Lena K, Müller, Matthias P, Itzen, Aymelt, Goody, Roger S
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 04.04.2012; Blackwell Publishing Ltd; Nature Publishing Group
• Subjects: Bacteria; Bacterial Proteins - metabolism; Cellular biology; GTP Phosphohydrolases - metabolism; GTPase-Activating Proteins - metabolism; Guanine Nucleotide Exchange Factors - metabolism; Legionella; Legionella pneumophila; Legionella pneumophila - metabolism; Membranes; Molecular biology; phosphocholination; Phosphorylcholine - metabolism; Proteins; rab GTP-Binding Proteins - metabolism; Rab1; Rab35

The Legionella pneumophila protein AnkX that is injected into infected cells by a Type IV secretion system transfers a phosphocholine group from CDP‐choline to a serine in the Rab1 and Rab35 GTPase Switch II regions. We show here that the consequences of phosphocholination on the interaction of Rab1...