Microtubule inhibitors identified through nonbiased screening enhance DNA transfection efficiency by delaying p62‐dependent ubiquitin recruitment

• Published in: Genes to cells : devoted to molecular & cellular mechanisms Vol. 26; no. 9; pp. 739 - 751
• Main Authors: Tsuchiya, Megumi, Ogawa, Hidesato, Watanabe, Kento, Koujin, Takako, Mori, Chie, Nunomura, Kazuto, Lin, Bangzhong, Tani, Akiyoshi, Hiraoka, Yasushi, Haraguchi, Tokuko
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.09.2021
• Subjects: Animals; Autophagy; Cells, Cultured; Colchicine; Colchicine - pharmacology; Deoxyribonucleic acid; DNA; Efficiency; Endocytosis; Fibroblasts - drug effects; Fibroblasts - metabolism; gene delivery; Gene expression; High-Throughput Screening Assays - methods; high‐throughput screening; Mice; Microtubules - drug effects; Microtubules - metabolism; p62; Phagocytosis; phosphorylation; Polystyrene; Sequestosome-1 Protein - metabolism; Small Molecule Libraries - pharmacology; Transfection; Transfection - methods; transfection efficiency; Tubulin Modulators - pharmacology; Ubiquitin; Ubiquitin - metabolism; Ubiquitination; Vinblastine; Vinblastine - pharmacology; gene delivery; autophagy; ubiquitination; high-throughput screening; p62; phosphorylation; transfection efficiency

Ectopic gene expression is an indispensable tool in biology and medicine, but is often limited by the low efficiency of DNA transfection. We previously reported that depletion of the autophagy receptor p62/SQSTM1 enhances DNA transfection efficiency by preventing the degradation of transfected DNA....