Proteomics analysis of rice seedling responses to ovine saliva

• Published in: Journal of plant physiology Vol. 168; no. 5; pp. 500 - 509
• Main Authors: Fan, Weihong, Cui, Weitong, Li, Xiaofeng, Chen, Shuangyan, Liu, Gongshe, Shen, Shihua
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 15.03.2011; Elsevier
• Subjects: 2-DE; adenosine triphosphate; Animals; Base Sequence; Biological and medical sciences; defoliation; Deposition; DNA Primers; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; gene expression regulation; genes; genetics; glyceraldehyde-3-phosphate dehydrogenase; grasslands; Grazing; H-transporting ATP synthase; MALDI-TOF; Oryza; Oryza - physiology; Ovine saliva; Pathways; peroxiredoxin; physiology; Plant physiology and development; protein synthesis; Proteins; Proteomics; regrowth; Reverse Transcriptase Polymerase Chain Reaction; ribulose-bisphosphate carboxylase; Rice; RNA, Messenger; RNA, Messenger - genetics; RT-PCR; Saliva; seedlings; Sheep; shoots; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Survival; transcription (genetics); CBB; MALDI-TOF; GBSSI; 2D-PAGE; 2-DE; IEF; RuBisCO; MALDI-TOF MS; SDS-PAGE; Ovine saliva; RT-PCR; CAT; Prx; ROS; GAPDH; Rice; Monocotyledones; Plant juvenile growth stage; Matrix assisted laser desorption ionization; Cereal crop; Response; Oryza sativa; Plant physiology; Gramineae; Analysis; Angiospermae; Proteomics; Spermatophyta; Reverse transcription polymerase chain reaction
• DOI: 10.1016/j.jplph.2010.08.012
• ISSN: 0176-1617; 1618-1328

Grazing is accompanied by a multitude of processes including wounding, saliva deposition, and defoliation. Previous studies have focused on the effects of the grazing or clipping intensity on plant regrowth, survival, and composition in the grassland. However, the impact of saliva deposition on plants is poorly understood. In this study, rice was used as a model plant to study the differentially expressed proteins after ovine saliva treatment. The shoots of 2-week-old seedlings were crosscut and the lower parts were daubed with ovine saliva at the cut surface. After 2, 6, 12 and 24 h, proteomics analysis was performed using proteins extracted from the saliva-treated shoots. The results showed that proteins involved in multiple pathways were differentially expressed in response to ovine saliva, including catalase (CAT), peroxiredoxin (Prx), ATP synthase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Moreover, real-time quantitative reverse-transcription-PCR (RT-PCR) data showed that most of the genes were also regulated at the transcript level. Our results indicate the ovine saliva induces an early response in the rice seedling by stress-related pathways. This study provides information about the response of rice seedlings to ovine saliva at the protein level.