Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells

• Published in: Investigative ophthalmology & visual science Vol. 55; no. 10; pp. 6953 - 6959
• Main Authors: Nakatsu, Martin N, González, Sheyla, Mei, Hua, Deng, Sophie X
• Format: Journal Article
• Language: English
• Published: United States The Association for Research in Vision and Ophthalmology 02.10.2014
• Subjects: Adult; Aged; Cell Differentiation; Cells, Cultured; Epithelium, Corneal - cytology; Female; Humans; Limbus Corneae - cytology; Male; Mesenchymal Stromal Cells - physiology; Middle Aged; Stem Cells - cytology; Young Adult; stem cells; fibroblasts; stem cell niche; mesenchymal; limbus; limbal stem cells; limbal stem cell deficiency; cornea epithelium

We tested the viability of human limbal mesenchymal cells (LMCs) to support the expansion of human corneal epithelial stem/progenitor cells (LSCs). Human LMCs were isolated from sclerocorneal tissue using collagenase A. Primary limbal epithelial cells (LECs) in the form of single cell suspension or...