Retinoic acid directs neuronal differentiation of human pluripotent stem cell lines in a non-cell-autonomous manner

• Published in: Differentiation (London) Vol. 80; no. 1; pp. 20 - 30
• Main Authors: Tonge, Peter D., Andrews, Peter W.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.07.2010
• Subjects: Antineoplastic Agents - pharmacology; Blotting, Western; Carcinoma, Embryonal - drug therapy; Carcinoma, Embryonal - metabolism; Carcinoma, Embryonal - pathology; Cell Cycle; Cell Differentiation - drug effects; Cell Proliferation; Differentiation; Embryonal Carcinoma Stem Cells - drug effects; Embryonal Carcinoma Stem Cells - metabolism; Flow Cytometry; Fluorescent Antibody Technique; Humans; Neurogenesis; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; NTERA2; Pluripotent Stem Cells - drug effects; Pluripotent Stem Cells - metabolism; Retinoic acid; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Signal Transduction; Stem cell; Tretinoin - pharmacology; Differentiation; Neurogenesis; Retinoic acid; Stem cell; NTERA2

Differentiation of human embryonic stem (ES) cells and embryonal carcinoma (EC) cells provides an in vitro model to study the process of neuronal differentiation. Retinoic acid (RA) is frequently used to promote neural differentiation of pluripotent cells under a wide variety of culture conditions....