A novel high-throughput multi-parameter flow cytometry based method for monitoring and rapid characterization of microbiome dynamics in anaerobic systems

• Published in: Bioresource technology Vol. 220; pp. 566 - 571
• Main Authors: Dhoble, Abhishek S., Bekal, Sadia, Dolatowski, William, Yanz, Connor, Lambert, Kris N., Bhalerao, Kaustubh D.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2016
• Subjects: Anaerobic digestion; Anaerobiosis; Autofluorescence; Automation; Bioreactors - microbiology; Cluster Analysis; Databases as Topic; DNA, Ribosomal Spacer - genetics; Flow cytometry; Flow Cytometry - methods; Labe free monitoring; Microbial community; Microbial Consortia - genetics; Microbial dynamics; Microbiome characterization; Microbiota - genetics; Time Factors; Flow cytometry; Labe free monitoring; Microbiome characterization; Autofluorescence; Microbial dynamics; Anaerobic digestion; Microbial community

•Flow cytometry can facilitate rapid characterization of dynamics of complex microbiomes.•Flow cytometry is comparable to traditional culture-independent techniques like ARISA.•Flow cytometry is sensitive to lab scale to industrial scale anaerobic digesters. A novel multidimensional flow cytometry b...