Hydrophobic handoff for direct delivery of peroxisome tail-anchored proteins

• Published in: Nature communications Vol. 5; no. 1; p. 5790
• Main Authors: Chen, Yinxiao, Pieuchot, Laurent, Loh, Rachel Ann, Yang, Jing, Kari, Teuku Mahfuzh Aufar, Wong, Jie Yun, Jedd, Gregory
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.12.2014; Nature Publishing Group
• Subjects: 631/45; 631/80; Amino Acid Sequence; Animals; Epithelial Cells - cytology; Epithelial Cells - metabolism; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression; Genes, Reporter; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humanities and Social Sciences; Humans; Hydrophobic and Hydrophilic Interactions; Kidney; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular Sequence Data; multidisciplinary; Neurospora crassa - genetics; Neurospora crassa - metabolism; Peroxisomes - chemistry; Peroxisomes - metabolism; Protein Binding; Protein Interaction Domains and Motifs; Protein Transport; Rats; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Red Fluorescent Protein; Science; Science (multidisciplinary); Sequence Alignment; Sequence Homology, Amino Acid

Tail-anchored (TA) proteins are inserted into membranes post-translationally through a C-terminal transmembrane domain (TMD). The PEX19 protein binds peroxisome TA proteins in the cytoplasm and delivers them to the membrane through the PEX3 receptor protein. An amphipathic segment in PEX19 promotes...