Sensitive immunoassays for human and rat GMFB and GMFG, tissue distribution and age-related changes

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects Vol. 1670; no. 3; pp. 208 - 216
• Main Authors: Inagaki, Masaaki, Aoyama, Mineyoshi, Sobue, Kazuya, Yamamoto, Naoki, Morishima, Tetsuro, Moriyama, Akihiko, Katsuya, Hirotada, Asai, Kiyofumi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24.02.2004; Elsevier BV
• Subjects: Adult; Age-related change; Aging; Aging - blood; Aging - metabolism; Animals; Cloning, Molecular; Enzyme immunoassay; Glia Maturation Factor; Glia Maturation Factor - analysis; Glia Maturation Factor - blood; Glia Maturation Factor - metabolism; Glia maturation factor beta; Glia maturation factor gamma; Humans; Immunoenzyme Techniques; Immunoenzyme Techniques - methods; Middle Aged; Rats; Rats, Wistar; Reproducibility of Results; Tissue Distribution; Glia maturation factor beta; Age-related change; Tissue distribution; Glia maturation factor gamma; Enzyme immunoassay

We developed sensitive and specific two-site enzyme immunoassays (EIA) for glia maturation factor beta (GMFB) and gamma (GMFG) using specific antibodies raised in rabbits. These assay systems enabled us to identify GMFB and GMFG (GMFs) in both human and rat samples and they were used to investigate...