Microhomology-mediated circular DNA formation from oligonucleosomal fragments during spermatogenesis

• Published in: eLife Vol. 12
• Main Authors: Hu, Jun, Zhang, Zhe, Xiao, Sai, Cao, Yalei, Chen, Yinghong, Weng, Jiaming, Jiang, Hui, Li, Wei, Chen, Jia-Yu, Liu, Chao
• Format: Journal Article
• Language: English
• Published: Cambridge eLife Sciences Publications Ltd 17.10.2023; eLife Sciences Publications, Ltd
• Subjects: Bioinformatics; Biosynthesis; Cell death; Circular DNA; Developmental Biology; DNA fragmentation; Euchromatin; extrachromosomal circular DNA; Genetics and Genomics; Genomes; Heterochromatin; Meiosis; microhomology; Mitochondrial DNA; Morphology; Quality control; Recombination hot spots; Sperm; Spermatogenesis

The landscape of extrachromosomal circular DNA (eccDNA) during mammalian spermatogenesis, as well as the biogenesis mechanism, remains to be explored. Here, we revealed widespread eccDNA formation in human sperms and mouse spermatogenesis. We noted that germline eccDNAs are derived from oligonucleos...