Direct and Indirect Effects on Viral Translation and RNA Replication Are Required for AUF1 Restriction of Enterovirus Infections in Human Cells

• Published in: mBio Vol. 9; no. 5
• Main Authors: Ullmer, Wendy, Semler, Bert L
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 04.09.2018
• Subjects: AUF1; coxsackievirus; enterovirus; Enterovirus B, Human - physiology; Gene Knockdown Techniques; HEK293 Cells; HeLa Cells; Heterogeneous Nuclear Ribonucleoprotein D0; Heterogeneous-Nuclear Ribonucleoprotein D - genetics; Host Microbial Interactions; host restriction; Host-Pathogen Interactions; Humans; Internal Ribosome Entry Sites; IRES-mediated translation; Poliovirus - physiology; Protein Binding; Protein Biosynthesis; Protein Transport; Real-Time Polymerase Chain Reaction; RNA replication; Virus Replication; AUF1; RNA replication; enterovirus; coxsackievirus; host restriction; IRES-mediated translation; picornavirus; poliovirus

The cellular mRNA decay protein AUF1 acts as a restriction factor during infection by picornaviruses, including poliovirus, coxsackievirus, and human rhinovirus. AUF1 relocalizes from the nucleus to the cytoplasm during infection by these viruses due to the disruption of nucleocytoplasmic traffickin...