Genome-Wide Spectra of Transcription Insertions and Deletions Reveal That Slippage Depends on RNA:DNA Hybrid Complementarity

• Published in: mBio Vol. 8; no. 4
• Main Authors: Traverse, Charles C., Ochman, Howard
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 29.08.2017
• Subjects: Base Sequence; Buchnera; Buchnera - genetics; DNA Replication; DNA, Bacterial - chemistry; DNA-Directed RNA Polymerases - metabolism; elongation complex; error rates; Escherichia coli; Escherichia coli - genetics; Gene Expression Profiling; Genome, Bacterial; INDEL Mutation; Mutation; Nucleic Acid Heteroduplexes; Nucleotides; RNA polymerase; RNA, Bacterial - chemistry; Sequence Deletion; Transcription, Genetic; mutation; transcription; transcription slippage; Escherichia coli; RNA polymerase; error rates; Buchnera; elongation complex

Advances in sequencing technologies have enabled direct quantification of genome-wide errors that occur during RNA transcription. These errors occur at rates that are orders of magnitude higher than rates during DNA replication, but due to technical difficulties such measurements have been limited t...