Proteomics Analysis of the Interactome of N-myc Downstream Regulated Gene 1 and Its Interactions with the Androgen Response Program in Prostate Cancer Cells

• Published in: Molecular & cellular proteomics Vol. 6; no. 4; pp. 575 - 588
• Main Authors: Tu, Lan Chun, Yan, Xiaowei, Hood, Leroy, Lin, Biaoyang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2007; American Society for Biochemistry and Molecular Biology
• Subjects: Androgens - metabolism; Androgens - pharmacology; beta Catenin - metabolism; Cadherins - metabolism; Cell Cycle Proteins - chemistry; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Endoplasmic Reticulum - metabolism; Humans; Immunoprecipitation; Intracellular Signaling Peptides and Proteins - chemistry; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Male; Models, Biological; Neoplasms, Hormone-Dependent - etiology; Neoplasms, Hormone-Dependent - genetics; Neoplasms, Hormone-Dependent - metabolism; Phosphorylation; Prostatic Neoplasms - etiology; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Protein Binding; Protein Interaction Mapping; Proteomics; Tandem Mass Spectrometry

NDRG1 is known to play important roles in both androgen-induced cell differentiation and inhibition of prostate cancer metastasis. However, the proteins associated with NDRG1 function are not fully enumerated. Using coimmunoprecipitation and mass spectrometry analysis, we identified 58 proteins that...