Nongastric H-K-ATPase in rodent prostate: lobe-specific expression and apical localization

• Published in: American Journal of Physiology: Cell Physiology Vol. 282; no. 4; pp. C907 - C916
• Main Authors: Pestov, Nikolay B, Korneenko, Tatyana V, Adams, Gail, Tillekeratne, Manoranjani, Shakhparonov, Mikhail I, Modyanov, Nikolai N
• Format: Journal Article
• Language: English
• Published: United States 01.04.2002
• Subjects: Amino Acid Sequence; Animals; Apocrine Glands - enzymology; Apocrine Glands - secretion; Blotting, Western; Gene Expression Regulation, Enzymologic; H(+)-K(+)-Exchanging ATPase - analysis; H(+)-K(+)-Exchanging ATPase - genetics; H(+)-K(+)-Exchanging ATPase - metabolism; Immunohistochemistry; Male; Mice; Molecular Sequence Data; Potassium - metabolism; Prostate - enzymology; Prostate - secretion; Rats; RNA, Messenger - analysis; Sodium-Potassium-Exchanging ATPase - analysis; Sodium-Potassium-Exchanging ATPase - genetics; Sodium-Potassium-Exchanging ATPase - metabolism
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00258.2001

1  Department of Pharmacology, Medical College of Ohio, Toledo, Ohio 43614; and 2  Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117871, Russia The molecular basis of active ion transport in secretory glands such as the prostate is not well characterized. Rat nongastric H-K-ATPase is expressed at high levels in distal colon surface cell apical membranes and thus is referred to as "colonic." Here we show that the ATPase is expressed in rodent prostate complex in a lobe-specific manner. RT-PCR and Western blot analyses indicate that rat nongastric H-K-ATPase -subunit ( ng ) mRNA and protein are present in coagulating gland (anterior prostate) and lateral and dorsal prostate and absent from ventral lobe, whereas Na-K-ATPase -subunit is present in all lobes. RT-PCR analysis shows that Na-K-ATPase 4 and 3 and gastric H-K-ATPase -subunit are not present in significant amounts in all prostate lobes. Relatively low levels of Na-K-ATPase 2 were found in lateral, dorsal, and anterior lobes. ng protein expression is anteriodorsolateral: highest in coagulating gland, somewhat lower in dorsal lobe, and even lower in lateral lobe. Na-K-ATPase protein abundance has the reverse order: expression in ventral lobe is higher than in coagulating gland. ng protein abundance is higher in coagulating gland than distal colon membranes. Immunohistochemistry shows that in rat and mouse coagulating gland epithelium ng protein has an apical polarization and Na-K-ATPase 1 is localized in basolateral membranes. The presence of nongastric H-K-ATPase in rodent prostate apical membranes may indicate its involvement in potassium concentration regulation in secretions of these glands. ATP1AL1 ; ATP12A ; hydrogen-potassium-adenosinetriphosphatase; sodium-potassium-adenosinetriphosphatase; X-potassium-adenosinetriphosphatase; male accessory glands; potassium transport