Evaluation of techniques for enrichment and isolation of Escherichia coli O157:H7 from artificially contaminated sprouts

• Published in: International journal of food microbiology Vol. 71; no. 1; pp. 87 - 92
• Main Authors: Weagant, Stephen D, Bound, Andrew J
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.12.2001; Elsevier
• Subjects: Agar; alfalfa; Biological and medical sciences; Cefixime; cell culture; Colony Count, Microbial; Colony Count, Microbial - methods; Culture Media; E. coli O157:H7; Enrichment methods; Escherichia coli; Escherichia coli O157; Escherichia coli O157 - isolation & purification; food contamination; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; genetic variation; Germination; Immunomagnetic separation; Immunomagnetic Separation - methods; isolation & purification; lentils; Medicago sativa; Medicago sativa - microbiology; methods; microbial contamination; microbiology; radishes; Seeds; Seeds - microbiology; Sensitivity and Specificity; sprouts (food); Immunomagnetic separation; E. coli O157:H7; Enrichment methods; Enrichment; Immunomagnetic method; Process improvement; Escherichia coli; Pathogen strain; Bacteria; Isolation; Foodstuff; Method; Biological contamination; Enterobacteriaceae

Because sprouted seed products are kept wet during and after production, have high levels of nutrients, and a neutral pH, they are subject to the outgrowth of pathogens such as Escherichia coli O157:H7. For these same reasons, these products also contain high levels of heterotrophic organisms and in...