Hepatic and renal cytochrome P-450s in spontaneously hypertensive rats

• Published in: Biochimica et biophysica acta Vol. 1074; no. 1; pp. 209 - 213
• Main Authors: Imaoka, Susumu, Funae, Yoshihiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.05.1991; Elsevier
• Subjects: Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Blotting, Western; Cardiology. Vascular system; Cytochrome P-450; Cytochrome P-450 Enzyme System - metabolism; Experimental diseases; Hydroxylation; Hypertension; Hypertension - enzymology; Isoenzymes - metabolism; Kidney - enzymology; Male; Medical sciences; Microsomes - enzymology; Microsomes, Liver - enzymology; Rat hepatic microsome; Rat renal microsome; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Testosterone - metabolism; Western blotting; Hypertension; Rat hepatic microsome; Western blotting; Cytochrome P-450; Rat renal microsome; Animal model; Hemoprotein; Rat; Cytochrome P450; Liver; Rodentia; Microsome; Kidney; Vertebrata; Mammalia; Enzymatic activity; Immunoblotting assay; Quantitative analysis
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(91)90063-M

The differences in the levels of cytochrome P-450s in hepatic and renal microsomes between spontaneously hypertensive rats (SHR) and normotensive control rats (Wistar Kyoto rats, WKY) were investigated by Western blotting with a specific antibody. Differences in the metabolic activity of the microsomes were also studied. In hepatic microsomes, the content of P450 PB-1 (IIIA2) was 140% higher in SHR than in WKY and the content of P450 IF-3 (IIA1) in SHR was one-seventh that in WKY. The differences reflected the increase in testosterone 6β-hydroxylation activity and decrease in testosterone 7α-hydroxylation activity in hepatic microsomes of SHR. The level of P450 K-5 (IVA2) in hepatic microsomes of SHR was 4-times that in microsomes of WKY. The levels of other cytochrome P-450s in SHR were not very different from those in WKY. In renal microsomes, the levels of three renal cytochrome P-450s, P450 K-2, K-4, and K-5, were measured. The level of P450 K-5 (fatty acid ω-hydroxylase) in SHR was 50% higher than that in WKY and the difference reflected the increase in lauric acid ω- and (ω − 1)-hydroxylation activities of the renal microsomes of SHR. The levels of P450 K-2 and K-4 did not differ in both rats.