Role of karyopherin nuclear transport receptors in nuclear transport by nuclear trafficking peptide

• Published in: Experimental cell research Vol. 409; no. 1; p. 112893
• Main Authors: Teratake, Yoichi, Kimura, Yayoi, Ishizaka, Yukihito
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021
• Subjects: 60 APPLIED LIFE SCIENCES; ABSORPTION SPECTROSCOPY; Active Transport, Cell Nucleus - physiology; AIDS VIRUS; AMINO ACIDS; beta Karyopherins - metabolism; CELL CULTURES; Cell Line, Tumor; Cell Nucleus - metabolism; CULTURE MEDIA; Cytoplasm - metabolism; FLUORESCENCE; Green Fluorescent Proteins - metabolism; HeLa Cells; HIV-1; HUMAN POPULATIONS; Humans; Importin b1; Karyopherins - metabolism; LIQUID COLUMN CHROMATOGRAPHY; MASS SPECTROSCOPY; Nuclear cargo; Nuclear Localization Signals - metabolism; Nuclear trafficking peptide; PEPTIDES; RECEPTORS; Receptors, Cytoplasmic and Nuclear - metabolism; RNA; Transportin 1; Vpr; vpr Gene Products, Human Immunodeficiency Virus - metabolism; HIV-1; Vpr; Importin b1; Nuclear trafficking peptide; Transportin 1; Nuclear cargo

Nuclear trafficking peptide (NTP), a cell-penetrating peptide (CPP) composed of 10 amino acids (aa) (RIFIHFRIGC), has potent nuclear trafficking activity. Recently, we established a protein-based cell engineering system by using NTP, but it remained elusive how NTP functions as a CPP with nuclear or...