Characterization of multimodal hydrophobic interaction chromatography media useful for isolation of green fluorescent proteins with small structural differences

• Published in: Journal of molecular recognition Vol. 22; no. 2; pp. 104 - 109
• Main Authors: Becker, Kristian, Hallgren, Elisabeth, Carredano, Enrique, Palmgren, Ronnie, Bülow, Leif
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.03.2009
• Subjects: Amino Acid Substitution; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; Biologi; Biological Sciences; Chromatography, Liquid - methods; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Library; green fluorescent protein; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - isolation & purification; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; hydrophobic interaction chromatography; Natural Sciences; Naturvetenskap; pH-responsive; polymer ligand; Protein Conformation; tyrosine tag

Hydrophobic interaction chromatography (HIC) has been developed as a powerful technique for separating and purifying proteins. In this study, we have characterized the ability of new multimodal pH‐HIC media to resolve proteins with only small differences in their primary structures. This was done by...