Submerged monoxenic culture of the entomopathogenic nematode Steinernema carpocapsae in an internal-loop airlift bioreactor using two configurations of the inner tube

• Published in: Biotechnology and bioengineering Vol. 98; no. 1; pp. 167 - 176
• Main Authors: Chavarría-Hernández, Norberto, Sanjuan-Galindo, René, Rodríguez-Pastrana, Blanca-Rosa, Medina-Torres, Luis, Rodríguez-Hernández, Adriana-Inés
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2007; Wiley
• Subjects: Agave; Air Movements; airlift bioreactors; Animals; biocontrol; bioinsecticides; Biological and medical sciences; Bioreactors; Biotechnology; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cell Proliferation; Coculture Techniques - instrumentation; Coculture Techniques - methods; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; hydrodynamics; Nematoda; Nematoda - growth & development; Nematoda - microbiology; oxygen transfer; Steinernema carpocapsae; submerged culture; Symbiosis - physiology; Xenorhabdus - physiology; Xenorhabdus nematophila; Entomopathogen; Airlift reactor; Biological control; Oxygen transfer; airlift bioreactors; Hydrodynamics; bioinsecticides; Steinernema carpocapsae; Bioreactor; Submerged culture; Helmintha; Nemathelminthia; Biopesticide; Invertebrata; Nematoda; biocontrol; Microbial insecticide

This article reports the submerged culture of the entomopathogenic nematode Steinernema carpocapsae and its symbiotic bacterium, Xenorhabdus nematophila, within an internal‐loop airlift bioreactor. Two configurations of the inner cylinder were tested: a standard draft tube (SDT) and a static mixer (...