Biochemical and functional differences between eosinophils from animal species and man

Eosinophils were isolated from peritoneal lavages of repeated horse serum‐injected guinea pigs or rhesus monkeys and from peripheral blood of normal human donors. Eicosanoid metabolism and chemotaxis by these cells were studied by in vitro techniques. Upon calcium ionophore stimulation guinea pig eo...

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Published inJournal of leukocyte biology Vol. 50; no. 2; pp. 140 - 150
Main Authors Sun, F.F., Crittenden, N.J., Czuk, C.I., Taylor, B.M., Stout, B.K., Johnson, H.G.
Format Journal Article
LanguageEnglish
Published Bethesda, MD Society for Leukocyte Biology 01.08.1991
Federation of American Societies for Experimental Biology
Subjects
Animals
Arachidonic Acids - blood
Biological and medical sciences
Calcimycin - pharmacology
calcium ionophore
chemotaxis
Chemotaxis, Leukocyte
eicosanoid metabolism
Eicosanoids - blood
eosinophils
Eosinophils - drug effects
Eosinophils - metabolism
Eosinophils - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
guinea pig
Guinea Pigs
Humans
Immunobiology
In Vitro Techniques
Leukotriene B4 - blood
Macaca mulatta
Myeloid cells: ontogeny, maturation, markers, receptors
Polynuclears
Species Specificity
Thromboxane B2 - blood
Human
Calcium
Cell population
Animal
Arachidonic acid derivatives
Granulocyte
Stimulation
Biosynthesis
Metabolism
Chemotaxis
Eosinophil
Comparative study
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Summary:Eosinophils were isolated from peritoneal lavages of repeated horse serum‐injected guinea pigs or rhesus monkeys and from peripheral blood of normal human donors. Eicosanoid metabolism and chemotaxis by these cells were studied by in vitro techniques. Upon calcium ionophore stimulation guinea pig eosinophils released thromboxane B2 (TXB2) and leukotriene B4 (LTB4) while monkey and human cells produced LTC4, LTB4, and 5‐HETE. Guinea pig cells do not synthesize sulfidopeptide LTs, because they lack the specific LTA4 glutathione S‐transferase. Guinea pig eosinophils exhibit maximal chemotactic responses to LTB4, zymosan activated plasma, and human recombinant C5a, while producing only a negligible response to platelet activating factor (PAF). Monkey and human cells responded maximally to PAF, but exhibit only a weak response to LTB4. These results suggest that the guinea pig eosinophils differ from monkey and human eosinophils in both the synthetic capacity and functional chemotaxis responses to lipid mediators.
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ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.50.2.140