Development of Genetically Encoded Fluorescent KSR1-Based Probes to Track Ceramides during Phagocytosis

• Published in: International journal of molecular sciences Vol. 25; no. 5; p. 2996
• Main Authors: Girik, Vladimir, van Ek, Larissa, Dentand Quadri, Isabelle, Azam, Maral, Cruz Cobo, María, Mandavit, Marion, Riezman, Isabelle, Riezman, Howard, Gavin, Anne-Claude, Nunes-Hasler, Paula
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.03.2024; MDPI
• Subjects: Biosensors; Ceramides; Ceramides - metabolism; Dendritic cells; DNA microarrays; Fluorescence; Fluorescent Dyes; Glycine; Kinases; Lipids; Localization; Mass spectrometry; Membranes; Phagocytosis; Plant lipids; Protein Kinases; Proteins; Scientific imaging; Signal Transduction - physiology; Canada; United Kingdom; Massachusetts; Germany; stromal interaction molecule 1 (STIM1); C6-ceramide; SET nuclear proto-oncogene; fumonisin B1; protein kinase c zeta (PRKCZ); sphingomyelinase; myriocin; phosphatase 2A inhibitor 2 (I2PP2A); Sec22b; lipidomics; kinase suppressor of ras 1 (KSR1)

Ceramides regulate phagocytosis; however, their exact function remains poorly understood. Here, we sought (1) to develop genetically encoded fluorescent tools for imaging ceramides, and (2) to use them to examine ceramide dynamics during phagocytosis. Fourteen enhanced green fluorescent protein (EGF...