Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis
The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD). ARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induc...
Saved in:
| Published in | Biochimica et biophysica acta Vol. 1850; no. 2; pp. 435 - 446 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
01.02.2015
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD).
ARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induced to undergo cell death by anoikis and the clearance of these cells by living hRPE/ARPE-19 or human monocyte-derived macrophages (HMDMs) in the presence or absence of TC was quantified by flow cytometry. TaqMan low-density gene expression array determining known markers of phagocytosis and loss-of-function studies on selected apopto-phagocytic genes was carried out in HMDM engulfing anoikic cells.
The glucocorticoid TC had a profound phagocytosis-enhancing effect on HMDM engulfing anoikic ARPE-19 or hRPE cells, causing a selective upregulation of the Mer tyrosine kinase (MERTK) receptor, while decreasing the expression of the AXL receptor tyrosine kinase and thrombospondin-1 (THSB-1). The key role of the MERTK could be demonstrated in HMDM engulfing dying cells using gene silencing as well as blocking antibodies. Similar pathways were found upregulated in living ARPE-19 engulfing anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in TC-treated HMDMs.
Specific agonists of the Mertk receptor may have a potential role as phagocytosis enhancers in the retina and serve as future targets for AMD therapy.
The use of Gas6 as enhancer of retinal phagocytosis via the MerTK receptor, alone or in combination with other specific ligands of the tyrosine kinase receptors' family may have a potential role in AMD therapy. |
|---|---|
| AbstractList | The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD).
ARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induced to undergo cell death by anoikis and the clearance of these cells by living hRPE/ARPE-19 or human monocyte-derived macrophages (HMDMs) in the presence or absence of TC was quantified by flow cytometry. TaqMan low-density gene expression array determining known markers of phagocytosis and loss-of-function studies on selected apopto-phagocytic genes was carried out in HMDM engulfing anoikic cells.
The glucocorticoid TC had a profound phagocytosis-enhancing effect on HMDM engulfing anoikic ARPE-19 or hRPE cells, causing a selective upregulation of the Mer tyrosine kinase (MERTK) receptor, while decreasing the expression of the AXL receptor tyrosine kinase and thrombospondin-1 (THSB-1). The key role of the MERTK could be demonstrated in HMDM engulfing dying cells using gene silencing as well as blocking antibodies. Similar pathways were found upregulated in living ARPE-19 engulfing anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in TC-treated HMDMs.
Specific agonists of the Mertk receptor may have a potential role as phagocytosis enhancers in the retina and serve as future targets for AMD therapy.
The use of Gas6 as enhancer of retinal phagocytosis via the MerTK receptor, alone or in combination with other specific ligands of the tyrosine kinase receptors' family may have a potential role in AMD therapy. The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD).BACKGROUNDThe apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD).ARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induced to undergo cell death by anoikis and the clearance of these cells by living hRPE/ARPE-19 or human monocyte-derived macrophages (HMDMs) in the presence or absence of TC was quantified by flow cytometry. TaqMan low-density gene expression array determining known markers of phagocytosis and loss-of-function studies on selected apopto-phagocytic genes was carried out in HMDM engulfing anoikic cells.METHODSARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induced to undergo cell death by anoikis and the clearance of these cells by living hRPE/ARPE-19 or human monocyte-derived macrophages (HMDMs) in the presence or absence of TC was quantified by flow cytometry. TaqMan low-density gene expression array determining known markers of phagocytosis and loss-of-function studies on selected apopto-phagocytic genes was carried out in HMDM engulfing anoikic cells.The glucocorticoid TC had a profound phagocytosis-enhancing effect on HMDM engulfing anoikic ARPE-19 or hRPE cells, causing a selective upregulation of the Mer tyrosine kinase (MERTK) receptor, while decreasing the expression of the AXL receptor tyrosine kinase and thrombospondin-1 (THSB-1). The key role of the MERTK could be demonstrated in HMDM engulfing dying cells using gene silencing as well as blocking antibodies. Similar pathways were found upregulated in living ARPE-19 engulfing anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in TC-treated HMDMs.RESULTSThe glucocorticoid TC had a profound phagocytosis-enhancing effect on HMDM engulfing anoikic ARPE-19 or hRPE cells, causing a selective upregulation of the Mer tyrosine kinase (MERTK) receptor, while decreasing the expression of the AXL receptor tyrosine kinase and thrombospondin-1 (THSB-1). The key role of the MERTK could be demonstrated in HMDM engulfing dying cells using gene silencing as well as blocking antibodies. Similar pathways were found upregulated in living ARPE-19 engulfing anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in TC-treated HMDMs.Specific agonists of the Mertk receptor may have a potential role as phagocytosis enhancers in the retina and serve as future targets for AMD therapy.CONCLUSIONSSpecific agonists of the Mertk receptor may have a potential role as phagocytosis enhancers in the retina and serve as future targets for AMD therapy.The use of Gas6 as enhancer of retinal phagocytosis via the MerTK receptor, alone or in combination with other specific ligands of the tyrosine kinase receptors' family may have a potential role in AMD therapy.GENERAL SIGNIFICANCEThe use of Gas6 as enhancer of retinal phagocytosis via the MerTK receptor, alone or in combination with other specific ligands of the tyrosine kinase receptors' family may have a potential role in AMD therapy. The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have relevance to development of age-related macular degeneration (AMD).ARPE-19 cells and primary human retinal pigment epithelium (hRPE) were induced to undergo cell death by anoikis and the clearance of these cells by living hRPE/ARPE-19 or human monocyte-derived macrophages (HMDMs) in the presence or absence of TC was quantified by flow cytometry. TaqMan low-density gene expression array determining known markers of phagocytosis and loss-of-function studies on selected apopto-phagocytic genes was carried out in HMDM engulfing anoikic cells.The glucocorticoid TC had a profound phagocytosis-enhancing effect on HMDM engulfing anoikic ARPE-19 or hRPE cells, causing a selective upregulation of the Mer tyrosine kinase (MERTK) receptor, while decreasing the expression of the AXL receptor tyrosine kinase and thrombospondin-1 (THSB-1). The key role of the MERTK could be demonstrated in HMDM engulfing dying cells using gene silencing as well as blocking antibodies. Similar pathways were found upregulated in living ARPE-19 engulfing anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in TC-treated HMDMs.Specific agonists of the Mertk receptor may have a potential role as phagocytosis enhancers in the retina and serve as future targets for AMD therapy.The use of Gas6 as enhancer of retinal phagocytosis via the MerTK receptor, alone or in combination with other specific ligands of the tyrosine kinase receptors' family may have a potential role in AMD therapy. |
| Author | Veréb, Zoltán Zahuczky, Gábor Petrovski, Goran Oláh, Brigitta Fésüs, László Kristóf, Endre Facskó, Andrea Albert, Réka Moe, Morten C. Szatmári-Tóth, Mária |
| Author_xml | – sequence: 1 givenname: Réka surname: Albert fullname: Albert, Réka – sequence: 2 givenname: Endre surname: Kristóf fullname: Kristóf, Endre – sequence: 3 givenname: Gábor surname: Zahuczky fullname: Zahuczky, Gábor – sequence: 4 givenname: Mária surname: Szatmári-Tóth fullname: Szatmári-Tóth, Mária – sequence: 5 givenname: Zoltán surname: Veréb fullname: Veréb, Zoltán – sequence: 6 givenname: Brigitta orcidid: 0000-0002-1795-3890 surname: Oláh fullname: Oláh, Brigitta – sequence: 7 givenname: Morten C. surname: Moe fullname: Moe, Morten C. – sequence: 8 givenname: Andrea surname: Facskó fullname: Facskó, Andrea – sequence: 9 givenname: László surname: Fésüs fullname: Fésüs, László – sequence: 10 givenname: Goran surname: Petrovski fullname: Petrovski, Goran |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25450174$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkc1u3CAUhVmkan7aN6gqlt3YAQx2prso6p-Uqpt0bV3wtYcJBhcYqfNUfcXiTiaLLloWIB2-c7jiXJIzHzwS8oazmjPeXu9qrWFCXwvGZZFqJtozcsEaJivJW3VOLlPasbLURr0k50JJxXgnL8ivh2hhNtYHVyJpxGnvIONAYQlLDtWyhSmYQ7aGlnyk-HOJmJINni6QM0afqPU0b5EahxDBG6RhpMPB-qnEZevB0cVOM_pMcbGFdLZIBp1LNb2lj3igMbg_rq8Y8-MpD_12TRvoaYaQbHpFXozgEr5-Oq_I948fHu4-V_ffPn25u72vjFQiV0ODXafbDWz0uqtxVBq0FOIGy_2NZA0ORoyNHlrOhR4LpDvToWi0VgC6uSLvjrlLDD_2mHI_27TODB7DPvWi_KXgLWfqvyhvy3Oik5sVffuE7vWMQ79EO0M89Kc-CiCPgIkhpYjjM8JZv1bd7_pj1f1a9aqWqovt_V82YzPk0lKOYN2_zb8B2pa36Q |
| CitedBy_id | crossref_primary_10_3390_life11070635 crossref_primary_10_1038_cddis_2016_133 crossref_primary_10_3390_life12070928 crossref_primary_10_1038_s41598_017_16524_w crossref_primary_10_3390_ijms20040926 crossref_primary_10_3390_ijms22041724 crossref_primary_10_3389_fmed_2021_794299 |
| Cites_doi | 10.1371/journal.pone.0021349 10.1182/blood-2006-02-001115 10.1167/iovs.12-9951 10.3109/09273948.2011.628432 10.1136/bmjopen-2012-002269 10.4049/jimmunol.178.9.5635 10.1016/S0161-6420(93)31466-1 10.1111/j.1755-3768.2010.02047.x 10.1189/jlb.0602325 10.1056/NEJMoa1102673 10.1016/j.ophtha.2012.04.015 10.1038/nm1103-1350 10.1097/00006982-200003000-00004 10.1038/nri957 10.1016/S1097-2765(04)00237-0 10.1016/j.mce.2007.05.019 10.1111/j.1755-3768.2011.02179.x 10.4049/jimmunol.1200662 10.1089/jop.2009.0131 10.1074/jbc.M107876200 10.1210/en.2006-0981 10.1146/annurev.pharmtox.47.120505.105225 10.1073/pnas.0704756104 10.1007/BF00920033 10.1084/jem.20041447 10.1083/jcb.42.2.392 10.4049/jimmunol.167.2.976 10.1038/35075603 10.1007/s00216-010-3648-1 10.1177/41.2.8419462 10.1159/000078778 10.1189/jlb.1005550 10.4049/jimmunol.0803503 10.1210/endo-119-1-62 10.1007/978-1-4419-1399-9_15 10.4049/jimmunol.1203017 10.1016/j.immuni.2005.05.002 10.1016/j.ophtha.2011.03.020 10.1016/j.imlet.2005.11.016 10.1038/sj.cdd.4402184 10.1136/bjo.2004.046391 10.1093/hmg/9.4.645 10.1111/acel.12072 10.1016/S1350-9462(01)00010-6 |
| ContentType | Journal Article |
| Copyright | Copyright © 2014. Published by Elsevier B.V. |
| Copyright_xml | – notice: Copyright © 2014. Published by Elsevier B.V. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 7S9 L.6 |
| DOI | 10.1016/j.bbagen.2014.10.026 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic AGRICOLA |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry Biology |
| EndPage | 446 |
| ExternalDocumentID | 25450174 10_1016_j_bbagen_2014_10_026 |
| Genre | Clinical Trial Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 23N 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8P~ 9JM AAEDT AAEDW AAHBH AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AATTM AAXKI AAXUO AAYWO AAYXX ABEFU ABFNM ABGSF ABMAC ABUDA ABWVN ABXDB ACDAQ ACIUM ACRLP ACRPL ACVFH ADBBV ADCNI ADEZE ADMUD ADNMO ADUVX AEBSH AEHWI AEIPS AEKER AEUPX AFJKZ AFPUW AFTJW AFXIZ AGCQF AGHFR AGQPQ AGRDE AGRNS AGUBO AGYEJ AHHHB AIEXJ AIGII AIIUN AIKHN AITUG AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU APXCP ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CITATION CS3 EBS EFJIC EJD EO8 EO9 EP2 EP3 FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HVGLF HZ~ IHE J1W KOM LX3 M41 MO0 N9A O-L O9- OAUVE OHT OZT P-8 P-9 PC. Q38 R2- ROL RPZ SBG SCC SDF SDG SDP SES SEW SPCBC SSH SSU SSZ T5K UQL WH7 WUQ XJT XPP ~G- -~X .55 .GJ AACTN AAYJJ ABJNI AFFNX AI. CGR CUY CVF ECM EIF F5P H~9 K-O MVM NPM RIG TWZ UHS VH1 X7M Y6R YYP ZE2 ZGI ~KM 7X8 7S9 L.6 |
| ID | FETCH-LOGICAL-c452t-d3e77b69a9bb69a5ff5bab4228e4528403edc2f3bd6112bfb69b7c7e23bb5aab3 |
| ISSN | 0304-4165 0006-3002 |
| IngestDate | Fri Jul 11 10:53:59 EDT 2025 Thu Jul 10 17:03:47 EDT 2025 Thu Apr 03 07:00:39 EDT 2025 Thu Apr 24 23:06:36 EDT 2025 Tue Jul 01 00:22:04 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | AMD Anoikis Gas6 MERTK Triamcinolone Phagocytosis |
| Language | English |
| License | Copyright © 2014. Published by Elsevier B.V. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c452t-d3e77b69a9bb69a5ff5bab4228e4528403edc2f3bd6112bfb69b7c7e23bb5aab3 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
| ORCID | 0000-0002-1795-3890 |
| OpenAccessLink | http://linkinghub.elsevier.com/retrieve/pii/S0304416514003626 |
| PMID | 25450174 |
| PQID | 1640327495 |
| PQPubID | 23479 |
| PageCount | 12 |
| ParticipantIDs | proquest_miscellaneous_2000216105 proquest_miscellaneous_1640327495 pubmed_primary_25450174 crossref_primary_10_1016_j_bbagen_2014_10_026 crossref_citationtrail_10_1016_j_bbagen_2014_10_026 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2015-02-01 |
| PublicationDateYYYYMMDD | 2015-02-01 |
| PublicationDate_xml | – month: 02 year: 2015 text: 2015-02-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Biochimica et biophysica acta |
| PublicationTitleAlternate | Biochim Biophys Acta |
| PublicationYear | 2015 |
| References | Scott (10.1016/j.bbagen.2014.10.026_bb0120) 2001; 411 Stahn (10.1016/j.bbagen.2014.10.026_bb0075) 2007; 275 Rodríguez-Muela (10.1016/j.bbagen.2014.10.026_bb0170) 2013; 12 Savill (10.1016/j.bbagen.2014.10.026_bb0020) 2002; 2 Bosch (10.1016/j.bbagen.2014.10.026_bb0185) 1993; 41 Tuo (10.1016/j.bbagen.2014.10.026_bb0155) 2012; 20 Jonas (10.1016/j.bbagen.2014.10.026_bb0070) 2005; 89 Mallavarapu (10.1016/j.bbagen.2014.10.026_bb0210) 2010; 664 Májai (10.1016/j.bbagen.2014.10.026_bb0040) 2006; 104 Ford (10.1016/j.bbagen.2014.10.026_bb0065) 2013; 3 Travis (10.1016/j.bbagen.2014.10.026_bb0165) 2007; 47 Klettner (10.1016/j.bbagen.2014.10.026_bb0225) 2012 Irschick (10.1016/j.bbagen.2014.10.026_bb0015) 2004; 36 Feng (10.1016/j.bbagen.2014.10.026_bb0205) 2002; 277 Petrovski (10.1016/j.bbagen.2014.10.026_bb0130) 2011; 89 Mallavarapu (10.1016/j.bbagen.2014.10.026_bb0215) 2010; vol. 664 Chakravarthy (10.1016/j.bbagen.2014.10.026_bb0230) 2012; 119 Gardai (10.1016/j.bbagen.2014.10.026_bb0035) 2006; 79 Lauber (10.1016/j.bbagen.2014.10.026_bb0030) 2004; 14 Floman (10.1016/j.bbagen.2014.10.026_bb0055) 1977; 16 Fujimoto (10.1016/j.bbagen.2014.10.026_bb0135) 2010; 397 Giles (10.1016/j.bbagen.2014.10.026_bb0100) 2001; 167 Zahuczky (10.1016/j.bbagen.2014.10.026_bb0085) 2011; 6 Zizzo (10.1016/j.bbagen.2014.10.026_bb0095) 2012; 189 Nandrot (10.1016/j.bbagen.2014.10.026_bb0190) 2004; 200 Johnen (10.1016/j.bbagen.2014.10.026_bb0125) 2012; 53 Penfold (10.1016/j.bbagen.2014.10.026_bb0150) 1990; 228 Green (10.1016/j.bbagen.2014.10.026_bb0005) 1993; 100 Ehrchen (10.1016/j.bbagen.2014.10.026_bb0110) 2007; 109 Stuart (10.1016/j.bbagen.2014.10.026_bb0025) 2005; 22 Heier (10.1016/j.bbagen.2014.10.026_bb0235) 2011; 118 Finnemann (10.1016/j.bbagen.2014.10.026_bb0200) 2006; vol. 572 Nandrot (10.1016/j.bbagen.2014.10.026_bb0195) 2007; 104 Tao (10.1016/j.bbagen.2014.10.026_bb0245) 2010; 26 Erwig (10.1016/j.bbagen.2014.10.026_bb0045) 2007; 15 Lewis (10.1016/j.bbagen.2014.10.026_bb0060) 1986; 119 Zizzo (10.1016/j.bbagen.2014.10.026_bb0090) 2013; 190 Tasker (10.1016/j.bbagen.2014.10.026_bb0080) 2006; 147 Young (10.1016/j.bbagen.2014.10.026_bb0160) 1969; 42 Seitz (10.1016/j.bbagen.2014.10.026_bb0220) 2007; 178 Martin (10.1016/j.bbagen.2014.10.026_bb0240) 2011; 364 Mosser (10.1016/j.bbagen.2014.10.026_bb0105) 2003; 73 McColl (10.1016/j.bbagen.2014.10.026_bb0115) 2009; 183 D'Cruz (10.1016/j.bbagen.2014.10.026_bb0180) 2000; 9 Danis (10.1016/j.bbagen.2014.10.026_bb0050) 2000; 20 Hageman (10.1016/j.bbagen.2014.10.026_bb0140) 2001; 20 Chaitin (10.1016/j.bbagen.2014.10.026_bb0175) 1983; 24 Kinnunen (10.1016/j.bbagen.2014.10.026_bb0145) 2012; 90 Forrester (10.1016/j.bbagen.2014.10.026_bb0010) 2003; 9 |
| References_xml | – volume: 6 start-page: e21349 year: 2011 ident: 10.1016/j.bbagen.2014.10.026_bb0085 article-title: Differentiation and glucocorticoid regulated apopto-phagocytic gene expression patterns in human macrophages. Role of Mertk in enhanced phagocytosis publication-title: PLoS One doi: 10.1371/journal.pone.0021349 – volume: 109 start-page: 1265 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0110 article-title: Glucocorticoids induce differentiation of a specifically activated, anti-inflammatory subtype of human monocytes publication-title: Blood doi: 10.1182/blood-2006-02-001115 – volume: 53 start-page: 4787 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0125 article-title: Sleeping Beauty transposon-mediated transfection of retinal and iris pigment epithelial cells publication-title: Invest. Ophthalmol. Vis. Sci. doi: 10.1167/iovs.12-9951 – volume: 20 start-page: 27 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0155 article-title: Genetics of immunological and inflammatory components in age-related macular degeneration publication-title: Ocul. Immunol. Inflamm. doi: 10.3109/09273948.2011.628432 – volume: 3 year: 2013 ident: 10.1016/j.bbagen.2014.10.026_bb0065 article-title: Current treatments in diabetic macular oedema: systematic review and meta-analysis publication-title: BMJ Open doi: 10.1136/bmjopen-2012-002269 – volume: 178 start-page: 5635 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0220 article-title: Macrophages and dendritic cells use different Axl/Mertk/Tyro3 receptors in clearance of apoptotic cells publication-title: J. Immunol. doi: 10.4049/jimmunol.178.9.5635 – volume: 100 start-page: 1519 year: 1993 ident: 10.1016/j.bbagen.2014.10.026_bb0005 article-title: Age-related macular degeneration histopathologic studies. The 1992 Lorenz E. Zimmerman Lecture publication-title: Ophthalmology doi: 10.1016/S0161-6420(93)31466-1 – volume: 89 start-page: e30 year: 2011 ident: 10.1016/j.bbagen.2014.10.026_bb0130 article-title: Clearance of dying ARPE-19 cells by professional and nonprofessional phagocytes in vitro — implications for age-related macular degeneration (AMD) publication-title: Acta Ophthalmol. doi: 10.1111/j.1755-3768.2010.02047.x – volume: 73 start-page: 209 year: 2003 ident: 10.1016/j.bbagen.2014.10.026_bb0105 article-title: The many faces of macrophage activation publication-title: J. Leukoc. Biol. doi: 10.1189/jlb.0602325 – volume: 364 start-page: 1897 year: 2011 ident: 10.1016/j.bbagen.2014.10.026_bb0240 article-title: Ranibizumab and bevacizumab for neovascular age-related macular degeneration publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1102673 – volume: 119 start-page: 1399 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0230 article-title: Ranibizumab versus bevacizumab to treat neovascular age-related macular degeneration: one-year findings from the IVAN randomized trial publication-title: Ophthalmology doi: 10.1016/j.ophtha.2012.04.015 – volume: 9 start-page: 1350 year: 2003 ident: 10.1016/j.bbagen.2014.10.026_bb0010 article-title: Macrophages eyed in macular degeneration publication-title: Nat. Med. doi: 10.1038/nm1103-1350 – volume: 20 start-page: 244 year: 2000 ident: 10.1016/j.bbagen.2014.10.026_bb0050 article-title: Intravitreal triamcinolone acetonide in exudative age-related macular degeneration publication-title: Retina doi: 10.1097/00006982-200003000-00004 – volume: 2 start-page: 965 year: 2002 ident: 10.1016/j.bbagen.2014.10.026_bb0020 article-title: A blast from the past: clearance of apoptotic cells regulates immune responses publication-title: Nat. Rev. Immunol. doi: 10.1038/nri957 – volume: 14 start-page: 277 year: 2004 ident: 10.1016/j.bbagen.2014.10.026_bb0030 article-title: Clearance of apoptotic cells: getting rid of the corpses publication-title: Mol. Cell doi: 10.1016/S1097-2765(04)00237-0 – volume: 275 start-page: 71 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0075 article-title: Molecular mechanisms of glucocorticoid action and selective glucocorticoid receptor agonists publication-title: Mol. Cell. Endocrinol. doi: 10.1016/j.mce.2007.05.019 – volume: 16 start-page: 69 year: 1977 ident: 10.1016/j.bbagen.2014.10.026_bb0055 article-title: Mechanism of steroid action in ocular inflammation: inhibition of prostaglandin production publication-title: Invest. Ophthalmol. Vis. Sci. – volume: 90 start-page: 299 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0145 article-title: Molecular mechanisms of retinal pigment epithelium damage and development of age-related macular degeneration publication-title: Acta Ophthalmol. doi: 10.1111/j.1755-3768.2011.02179.x – volume: 189 start-page: 3508 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0095 article-title: Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction publication-title: J. Immunol. doi: 10.4049/jimmunol.1200662 – volume: 26 start-page: 207 year: 2010 ident: 10.1016/j.bbagen.2014.10.026_bb0245 article-title: Intravitreal bevacizumab combined with intravitreal triamcinolone for therapy-resistant exudative age-related macular degeneration publication-title: J. Ocul. Pharmacol. Ther. doi: 10.1089/jop.2009.0131 – volume: 277 start-page: 17016 year: 2002 ident: 10.1016/j.bbagen.2014.10.026_bb0205 article-title: Mertk triggers uptake of photoreceptor outer segments during phagocytosis by cultured retinal pigment epithelial cells publication-title: J. Biol. Chem. doi: 10.1074/jbc.M107876200 – volume: 147 start-page: 5549 year: 2006 ident: 10.1016/j.bbagen.2014.10.026_bb0080 article-title: Minireview: rapid glucocorticoid signaling via membrane-associated receptors publication-title: Endocrinology doi: 10.1210/en.2006-0981 – volume: 47 start-page: 469 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0165 article-title: Diseases caused by defects in the visual cycle: retinoids as potential therapeutic agents publication-title: Annu. Rev. Pharmacol. Toxicol. doi: 10.1146/annurev.pharmtox.47.120505.105225 – start-page: 277 year: 2012 ident: 10.1016/j.bbagen.2014.10.026_bb0225 article-title: Mechanisms of Pathological VEGF Production in the Retina and Modification with VEGF-antagonists – volume: 104 start-page: 12005 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0195 article-title: Essential role for MFG-E8 as ligand for αvβ5 integrin in diurnal retinal phagocytosis publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0704756104 – volume: 228 start-page: 270 year: 1990 ident: 10.1016/j.bbagen.2014.10.026_bb0150 article-title: Autoantibodies to retinal astrocytes associated with age-related macular degeneration publication-title: Graefes Arch. Clin. Exp. Ophthalmol. doi: 10.1007/BF00920033 – volume: 200 start-page: 1539 year: 2004 ident: 10.1016/j.bbagen.2014.10.026_bb0190 article-title: Loss of synchronized retinal phagocytosis and age-related blindness in mice lacking αvβ5 integrin publication-title: J. Exp. Med. doi: 10.1084/jem.20041447 – volume: 42 start-page: 392 year: 1969 ident: 10.1016/j.bbagen.2014.10.026_bb0160 article-title: Participation of the retinal pigment epithelium in the rod outer segment renewal process publication-title: J. Cell Biol. doi: 10.1083/jcb.42.2.392 – volume: 167 start-page: 976 year: 2001 ident: 10.1016/j.bbagen.2014.10.026_bb0100 article-title: Glucocorticoid augmentation of macrophage capacity for phagocytosis of apoptotic cells is associated with reduced p130Cas expression, loss of paxillin/pyk2 phosphorylation, and high levels of active Rac publication-title: J. Immunol. doi: 10.4049/jimmunol.167.2.976 – volume: 411 start-page: 207 year: 2001 ident: 10.1016/j.bbagen.2014.10.026_bb0120 article-title: Phagocytosis and clearance of apoptotic cells is mediated by MER publication-title: Nature doi: 10.1038/35075603 – volume: vol. 664 start-page: 123 year: 2010 ident: 10.1016/j.bbagen.2014.10.026_bb0215 article-title: Neural Retina and MerTK-independent Apical Polarity of αvβ5 Integrin Receptors in the Retinal Pigment Epithelium – volume: 397 start-page: 571 year: 2010 ident: 10.1016/j.bbagen.2014.10.026_bb0135 article-title: Layer-by-layer assembly of small interfering RNA and poly(ethyleneimine) for substrate-mediated electroporation with high efficiency publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-010-3648-1 – volume: 41 start-page: 253 year: 1993 ident: 10.1016/j.bbagen.2014.10.026_bb0185 article-title: Phagocytosis of outer segments by retinal pigment epithelium: phagosome–lysosome interaction publication-title: J. Histochem. Cytochem. doi: 10.1177/41.2.8419462 – volume: 36 start-page: 200 year: 2004 ident: 10.1016/j.bbagen.2014.10.026_bb0015 article-title: Retinal pigment epithelial phagocytosis and metabolism differ from those of macrophages publication-title: Ophthalmic Res. doi: 10.1159/000078778 – volume: 79 start-page: 896 year: 2006 ident: 10.1016/j.bbagen.2014.10.026_bb0035 article-title: Recognition ligands on apoptotic cells: a perspective publication-title: J. Leukoc. Biol. doi: 10.1189/jlb.1005550 – volume: 183 start-page: 2167 year: 2009 ident: 10.1016/j.bbagen.2014.10.026_bb0115 article-title: Glucocorticoids induce protein S-dependent phagocytosis of apoptotic neutrophils by human macrophages publication-title: J. Immunol. doi: 10.4049/jimmunol.0803503 – volume: vol. 572 start-page: 499 year: 2006 ident: 10.1016/j.bbagen.2014.10.026_bb0200 article-title: Mertk Activation During RPE Phagocytosis in Vivo Requires αVβ5 Integrin – volume: 119 start-page: 62 year: 1986 ident: 10.1016/j.bbagen.2014.10.026_bb0060 article-title: Inhibition of prostaglandin synthesis by glucocorticoids in human endothelial cells publication-title: Endocrinology doi: 10.1210/endo-119-1-62 – volume: 24 start-page: 812 year: 1983 ident: 10.1016/j.bbagen.2014.10.026_bb0175 article-title: Defective ingestion of rod outer segments by cultured dystrophic rat pigment epithelial cells publication-title: Invest. Ophthalmol. Vis. Sci. – volume: 664 start-page: 123 year: 2010 ident: 10.1016/j.bbagen.2014.10.026_bb0210 article-title: Neural retina and MerTK-independent apical polarity of αvβ5 integrin receptors in the retinal pigment epithelium publication-title: Adv. Exp. Med. Biol. doi: 10.1007/978-1-4419-1399-9_15 – volume: 190 start-page: 5237 year: 2013 ident: 10.1016/j.bbagen.2014.10.026_bb0090 article-title: IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids publication-title: J. Immunol. doi: 10.4049/jimmunol.1203017 – volume: 22 start-page: 539 year: 2005 ident: 10.1016/j.bbagen.2014.10.026_bb0025 article-title: Phagocytosis: elegant complexity publication-title: Immunity doi: 10.1016/j.immuni.2005.05.002 – volume: 118 start-page: 1098 year: 2011 ident: 10.1016/j.bbagen.2014.10.026_bb0235 article-title: The 1-year results of CLEAR-IT 2, a phase 2 study of vascular endothelial growth factor trap-eye dosed as-needed after 12-week fixed dosing publication-title: Ophthalmology doi: 10.1016/j.ophtha.2011.03.020 – volume: 104 start-page: 94 year: 2006 ident: 10.1016/j.bbagen.2014.10.026_bb0040 article-title: Inflammation and the apopto-phagocytic system publication-title: Immunol. Lett. doi: 10.1016/j.imlet.2005.11.016 – volume: 15 start-page: 243 year: 2007 ident: 10.1016/j.bbagen.2014.10.026_bb0045 article-title: Clearance of apoptotic cells by phagocytes publication-title: Cell Death Differ. doi: 10.1038/sj.cdd.4402184 – volume: 89 start-page: 321 year: 2005 ident: 10.1016/j.bbagen.2014.10.026_bb0070 article-title: Diffuse diabetic macular oedema treated by intravitreal triamcinolone acetonide: a comparative, non-randomised study publication-title: Br. J. Ophthalmol. doi: 10.1136/bjo.2004.046391 – volume: 9 start-page: 645 year: 2000 ident: 10.1016/j.bbagen.2014.10.026_bb0180 article-title: Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat publication-title: Hum. Mol. Genet. doi: 10.1093/hmg/9.4.645 – volume: 12 start-page: 478 issue: 3 year: 2013 ident: 10.1016/j.bbagen.2014.10.026_bb0170 article-title: Balance between autophagic pathways preserves retinal homeostasis publication-title: Aging Cell doi: 10.1111/acel.12072 – volume: 20 start-page: 705 year: 2001 ident: 10.1016/j.bbagen.2014.10.026_bb0140 article-title: An integrated hypothesis that considers drusen as biomarkers of immune-mediated processes at the RPE-Bruch's membrane interface in aging and age-related macular degeneration publication-title: Prog. Retin. Eye Res. doi: 10.1016/S1350-9462(01)00010-6 |
| SSID | ssj0000595 ssj0025309 |
| Score | 2.1796715 |
| Snippet | The apopto-phagocytic gene expression patterns during clearance of dying cells in the retina and the effect of triamcinolone (TC) upon these processes have... |
| SourceID | proquest pubmed crossref |
| SourceType | Aggregation Database Index Database Enrichment Source |
| StartPage | 435 |
| SubjectTerms | agonists Anoikis - drug effects Anoikis - genetics Anti-Inflammatory Agents - pharmacology antibodies Antibodies, Neutralizing - pharmacology c-Mer Tyrosine Kinase cell death Cell Line epithelial cells Epithelial Cells - cytology Epithelial Cells - enzymology epithelium Eye Proteins - biosynthesis Eye Proteins - genetics Female flow cytometry gene expression Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Enzymologic - genetics gene silencing Gene Silencing - drug effects genes Humans ligands loss-of-function mutation macrophages Macrophages - cytology Macrophages - metabolism macular degeneration Macular Degeneration - drug therapy Macular Degeneration - enzymology Macular Degeneration - genetics Male phagocytosis Phagocytosis - drug effects Phagocytosis - genetics Proto-Oncogene Proteins - biosynthesis Proto-Oncogene Proteins - genetics receptor protein-tyrosine kinase Receptor Protein-Tyrosine Kinases - biosynthesis Receptor Protein-Tyrosine Kinases - genetics receptors retina Retinal Pigment Epithelium - cytology Retinal Pigment Epithelium - enzymology therapeutics triamcinolone Triamcinolone - pharmacology tyrosine |
| Title | Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25450174 https://www.proquest.com/docview/1640327495 https://www.proquest.com/docview/2000216105 |
| Volume | 1850 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JbtswECUc59Beiu5Jl4AFegtkWAul6OgGaYMG7iF10NwEkqJiJbEk2PKhPvSX-lH9kc6Qoiw3NbpcBIOkKFnzRA5Hbx4JeevCW-ArlzkqFqkTRDKAdy6QTuQKj2dHIYv1F9Pxp_D0Ivh4yS57vR8d1tKyFgO5-m1eyf9YFcrArpgl-w-WbTuFAvgN9oUjWBiOf2djuMZM5hgaL3D7E72tPHiQvCqrunSqKb8q5VeUZIUuFKr5G9arllPFSODC0hwl7h6hswfAeUx16hOmN6KrWuVXmjCgKkzfuMUIO0b7FwMYU2AEaPmJYzWvb2x_qpgaaoG9h3KRLzY-IOelnOaoVXCo6kORlybEwlHco50pRijApWeJc_NB_6at0oMTFr7ztazkCRIz2yg4ny7lykSFP-gzXVG2LOTPK17PTOk8dyamExNeGtti3o2GuMwSqDsjPHL5hr-M8EbctsGy1xmwAyOWcmciMTGN64EQ0Ap1ct1ggCxAk97fwVY10-CCdTaDwS1YT6st2dFW7ZBdD9YyXp_sjs7Ov5y1cQHmN0yk5tZthqemId69AdSvbrrcdKa2rJC0pzR5SB40Sxw6Mnh9RHqqeEzuHdudBZ-Q7xu4pS1u6R3cUsQtXeOWWtzSvKCAM9rilpYZ1bilDW5pg1u6xi01uKUjCriliFs8S-PW9mdxS7u4fUou3p9Mjk-dZuMQRwbMq53UV1EkwpjHAo8sy5jgAsXuFNQfBUNfpdLLfJGGsNwQGTQSkYyU5wvBOBf-M9Iv4AHsERrIoQ8NJUvdFPw4n8cyAh8_BBfHVT4L9olvLZDIRlUfN3e5TSx98joxJkzQhFgKJtwnTntWZVRl_tD-jTVuArbCp8ULVS4XiRvCv_GiIGbb22A2ngcruyG0eW6Q0V7VIunF1pqX5P76RXtF-vV8qV6DI16LA7Iz-OYeNHD-CSCU4jU |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Triamcinolone+regulated+apopto-phagocytic+gene+expression+patterns+in+the+clearance+of+dying+retinal+pigment+epithelial+cells.+A+key+role+of+Mertk+in+the+enhanced+phagocytosis&rft.jtitle=Biochimica+et+biophysica+acta&rft.au=Albert%2C+R%C3%A9ka&rft.au=Krist%C3%B3f%2C+Endre&rft.au=Zahuczky%2C+G%C3%A1bor&rft.au=Szatm%C3%A1ri-T%C3%B3th%2C+M%C3%A1ria&rft.date=2015-02-01&rft.issn=0006-3002&rft.volume=1850&rft.issue=2&rft.spage=435&rft_id=info:doi/10.1016%2Fj.bbagen.2014.10.026&rft_id=info%3Apmid%2F25450174&rft.externalDocID=25450174 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0304-4165&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0304-4165&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0304-4165&client=summon |