Flexible HIV-1 Biosensor Based on the Au/MoS2 Nanoparticles/Au Nanolayer on the PET Substrate

• Published in: Nanomaterials (Basel, Switzerland) Vol. 9; no. 8; p. 1076
• Main Authors: Shin, Minkyu, Yoon, Jinho, Yi, Chanyong, Lee, Taek, Choi, Jeong-Woo
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26.07.2019; MDPI
• Subjects: Acquired immune deficiency syndrome; AIDS; Antibodies; Antigens; Atomic force microscopy; Biocompatibility; Biosensors; Coatings; Cysteamine; Deoxyribonucleic acid; DNA; Electrochemistry; Fabrication; Fatigue testing machines; Fatigue tests; Flexibility; flexible biosensor; Glycoprotein gp120; Glycoproteins; Gold; Gold coatings; gp120; Graphene; HIV; HIV-1; Human immunodeficiency virus; Infections; Manufacturing; Methods; Microscopy; Molybdenum; Molybdenum disulfide; MoS2 nanoparticles; nanolayer; Nanomaterials; Nanoparticles; PET substrate; Polyethylene; Polyethylene terephthalate; Polymerase chain reaction; Polymers; Potassium; Quantum dots; Scanning electron microscopy; Sensors; Square waves; Substrates; Viruses; Voltammetry; Wearable technology; United Kingdom > UK; United States > US
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano9081076

An electrochemical flexible biosensor composed of gold (Au), molybdenum disulfide nanoparticles (MoS2 NPs), and Au (Au/MoS2/Au nanolayer) on the polyethylene terephthalate (PET) substrate is developed to detect envelope glycoprotein GP120 (gp120), the surface protein of HIV-1. To fabricate the nanolayer on the PET substrate, Au is sputter coated on the flexible PET substrate and MoS2 NPs are spin coated on Au, which is sputter coated once again with Au. The gp120 antibody is then immobilized on this flexible electrode through cysteamine (Cys) modified on the surface of the Au/MoS2/Au nanolayer. Fabrication of the biosensor is verified by atomic force microscopy, scanning electron microscopy, and cyclic voltammetry. A flexibility test is done using a micro-fatigue tester. Detection of the gp120 is measured by square wave voltammetry. The results indicate that the prepared biosensor detects 0.1 pg/mL of gp120, which is comparable with previously reported gp120 biosensors prepared even without flexibility. Therefore, the proposed biosensor supports the development of a nanomaterial-based flexible sensing platform for highly sensitive biosensors with flexibility for wearable device application.