The role of actin and myosin in ascidian sperm mitochondrial translocation

• Published in: Developmental biology Vol. 106; no. 2; pp. 307 - 314
• Main Authors: Lambert, Charles C., Lambert, Gretchen
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.12.1984; Elsevier
• Subjects: Actins - isolation & purification; Actins - physiology; Animals; Biological and medical sciences; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Invertebrates; Less representative or incertae sedis groups: acanthocephala, chaetognatha, gnathostomulida, lophophoria, merostomata, mesozoa, myxozoa, nematorhyncha, pararthropoda, placozoa, priapuloidea, prochordata, pycnogonida, rotifera; Male; Mitochondria - physiology; Mitochondria - ultrastructure; Myosins - isolation & purification; Myosins - physiology; Spermatozoa - physiology; Urochordata - physiology; Marine environment; Gamete; Intracellular movement; Mitochondria; Spermatozoa; Cell organelle; Myosin; Actins; Contractile protein; Prochordata; Invertebrata; Urochordata

Fertilization-related sperm mitochondrial movement occurs at a rate comparable to other actin-myosin-driven movements and is inhibited by cytochalasin B and N-ethyl maleimide in Ascidia ceratodes sperm. F-actin was demonstrated in the tails and mitochondria using NBD-phallacidin fluorescence. Both a...