The psychosis risk factor RBM12 encodes a novel repressor of GPCR/cAMP signal transduction

• Published in: The Journal of biological chemistry Vol. 299; no. 9; p. 105133
• Main Authors: Semesta, Khairunnisa M., Garces, Angelica, Tsvetanova, Nikoleta G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2023; American Society for Biochemistry and Molecular Biology
• Subjects: Adenylyl Cyclases - genetics; Adenylyl Cyclases - metabolism; Cyclic AMP - antagonists & inhibitors; Cyclic AMP - genetics; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - genetics; Cyclic AMP-Dependent Protein Kinases - metabolism; Gene Expression Regulation, Enzymologic - genetics; HEK293 Cells; Humans; Neurons - physiology; Psychotic Disorders - genetics; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Signal Transduction - genetics; BDNF; DMSO; GO; TBST; RRM; gRNA; PDE; DMEM; CREB; PKAcat; β2-AR; CRISPRi; hiPSC; RBM 12; GPCR; NGN2; NTC; RT-qPCR

RBM12 is a high-penetrance risk factor for familial schizophrenia and psychosis, yet its precise cellular functions and the pathways to which it belongs are not known. We utilize two complementary models, HEK293 cells and human iPSC-derived neurons, and delineate RBM12 as a novel repressor of the G...