The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain geno...

Full description

Saved in:
Bibliographic Details
Published ineLife Vol. 7
Main Authors Davis, Gregory M, Tu, Shikui, Anderson, Joshua WT, Colson, Rhys N, Gunzburg, Menachem J, Francisco, Michelle A, Ray, Debashish, Shrubsole, Sean P, Sobotka, Julia A, Seroussi, Uri, Lao, Robert X, Maity, Tuhin, Wu, Monica Z, McJunkin, Katherine, Morris, Quaid D, Hughes, Timothy R, Wilce, Jacqueline A, Claycomb, Julie M, Weng, Zhiping, Boag, Peter R
Format Journal Article
LanguageEnglish
Published Cambridge eLife Sciences Publications Ltd 21.12.2018
eLife Sciences Publications, Ltd
Subjects
22G RNAs
Biology
Biosynthesis
Caenorhabditis elegans
Chromatin
Chromosomes and Gene Expression
Gene expression
Genetics and Genomics
Genomes
Homeostasis
Meiosis
Proteins
Ribonucleic acid
RNA
RNA polymerase
RNA-binding protein
RNA-mediated interference
RNAi
small RNAs
TRIM-NHL
Australia
United States > US
Online AccessGet full text

Cover

Abstract Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
AbstractList Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Author Colson, Rhys N
Morris, Quaid D
Tu, Shikui
Lao, Robert X
Anderson, Joshua WT
Sobotka, Julia A
Wu, Monica Z
Weng, Zhiping
Davis, Gregory M
Seroussi, Uri
Claycomb, Julie M
Gunzburg, Menachem J
Hughes, Timothy R
Maity, Tuhin
McJunkin, Katherine
Francisco, Michelle A
Wilce, Jacqueline A
Ray, Debashish
Shrubsole, Sean P
Boag, Peter R
Author_xml – sequence: 1
  givenname: Gregory M
  surname: Davis
  fullname: Davis, Gregory M
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia, School of Health and Life Sciences, Federation University, Victoria, Australia
– sequence: 2
  givenname: Shikui
  surname: Tu
  fullname: Tu, Shikui
  organization: Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States
– sequence: 3
  givenname: Joshua WT
  surname: Anderson
  fullname: Anderson, Joshua WT
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
– sequence: 4
  givenname: Rhys N
  surname: Colson
  fullname: Colson, Rhys N
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
– sequence: 5
  givenname: Menachem J
  surname: Gunzburg
  fullname: Gunzburg, Menachem J
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
– sequence: 6
  givenname: Michelle A
  surname: Francisco
  fullname: Francisco, Michelle A
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 7
  givenname: Debashish
  surname: Ray
  fullname: Ray, Debashish
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 8
  givenname: Sean P
  surname: Shrubsole
  fullname: Shrubsole, Sean P
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
– sequence: 9
  givenname: Julia A
  surname: Sobotka
  fullname: Sobotka, Julia A
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 10
  givenname: Uri
  surname: Seroussi
  fullname: Seroussi, Uri
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 11
  givenname: Robert X
  surname: Lao
  fullname: Lao, Robert X
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 12
  givenname: Tuhin
  surname: Maity
  fullname: Maity, Tuhin
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 13
  givenname: Monica Z
  surname: Wu
  fullname: Wu, Monica Z
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 14
  givenname: Katherine
  surname: McJunkin
  fullname: McJunkin, Katherine
  organization: Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States
– sequence: 15
  givenname: Quaid D
  surname: Morris
  fullname: Morris, Quaid D
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 16
  givenname: Timothy R
  surname: Hughes
  fullname: Hughes, Timothy R
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 17
  givenname: Jacqueline A
  surname: Wilce
  fullname: Wilce, Jacqueline A
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
– sequence: 18
  givenname: Julie M
  surname: Claycomb
  fullname: Claycomb, Julie M
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Canada
– sequence: 19
  givenname: Zhiping
  surname: Weng
  fullname: Weng, Zhiping
  organization: Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States
– sequence: 20
  givenname: Peter R
  orcidid: 0000-0002-0889-0859
  surname: Boag
  fullname: Boag, Peter R
  organization: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
BookMark eNptklGLEzEQx4OceOd5T36BgC-CbE2yySZ5EY6iXqGecFTwLabJbJuyTWqyVe7bm7YneId5yTDzm38mw_8lOospAkKvKZlIIfh7mIceJq3gUj1DF4wI0hDFv5_9E5-jq1I2pB7JlaL6BTpviajdVF2gH4s14MXd7EtzezPHu5xGCBHXuGE4FGyxS01v3ZgyrvmxwnHvBrAZ2-hxSVs7Bofvbq8D3tlx_dvelwM4nWAYYGVjeYWe93YocPVwX6Jvnz4upjfN_Ovn2fR63jgu2NgovZRSdR1RQkvXOdp73rZSUeb9UmvLgHnVd55rzZkgXvLeuaUAwbjorIf2Es1Ouj7ZjdnlsLX53iQbzDGR8srYXGcdwBDmpNQdpyAVl75VTHEL3tmeC95KUrU-nLR2--W2FiCO2Q6PRB9XYlibVfplulZQSngVePsgkNPPPZTRbENxMAw2QtoXw6jQWtXRdUXfPEE3aZ9jXVWlJK-kZLRS9ES5nErJ0BsXxrr6dHg_DIYSc7CDOdrBHO1Qe9496fn7gf_RfwDL27QY
CitedBy_id crossref_primary_10_1016_j_jhazmat_2020_125035
crossref_primary_10_1016_j_ydbio_2022_08_010
crossref_primary_10_1016_j_devcel_2024_12_016
crossref_primary_10_1093_g3journal_jkad092
crossref_primary_10_1016_j_semcdb_2021_11_018
crossref_primary_10_1016_j_bbadis_2020_166066
crossref_primary_10_1515_hsz_2019_0158
crossref_primary_10_1002_bies_202200220
crossref_primary_10_15252_embr_202358116
crossref_primary_10_1093_nar_gkab619
Cites_doi 10.1371/journal.pgen.1000624
10.1534/genetics.117.203174
10.1016/j.cell.2012.06.015
10.1101/gad.290155.116
10.1242/dev.00914
10.1371/journal.pgen.1005580
10.1016/j.devcel.2013.11.016
10.1186/s13072-016-0052-x
10.1534/genetics.116.198812
10.1016/j.celrep.2015.09.068
10.1016/j.cell.2016.02.040
10.3389/fgene.2014.00416
10.1002/pro.5560041120
10.1007/978-1-4614-5398-7_3
10.1016/j.cell.2008.12.024
10.1016/j.cell.2004.12.035
10.1016/j.ydbio.2008.06.037
10.1101/gad.236513.113
10.1007/s10577-013-9383-7
10.1093/nar/gku1077
10.1016/j.ymeth.2016.12.003
10.1002/iub.1039
10.1242/dev.075986
10.1016/j.devcel.2013.11.014
10.1534/g3.114.012351
10.1534/g3.117.300166
10.1038/nature07014
10.1534/genetics.114.170423
10.1083/jcb.200506124
10.1038/nbt.1550
10.1016/j.cell.2009.09.012
10.1038/sj.emboj.7601910
10.1101/gad.1119403
10.1111/j.1365-2443.2007.01111.x
10.1038/27579
10.4161/worm.28641
10.1895/wormbook.1.170.1
10.1016/j.devcel.2017.04.023
10.1016/j.molcel.2009.09.020
10.1038/nature12311
10.1016/j.cell.2013.11.032
10.1016/j.cub.2014.02.060
10.1371/journal.pgen.1004533
10.1093/genetics/153.3.1245
10.1083/jcb.200801183
10.1093/bioinformatics/btp120
10.1186/gb-2009-10-3-r25
10.1101/gad.193904.112
10.1101/gad.870801
10.1016/j.cub.2008.05.009
10.1016/j.cell.2005.11.036
10.1534/genetics.114.168831
10.1242/dev.128.17.3221
10.1016/j.cell.2009.09.014
10.1186/s13059-015-0659-4
10.1101/cshperspect.a002774
10.1016/j.jmb.2005.09.068
10.1016/j.cell.2012.06.018
10.1016/j.devcel.2015.07.009
10.1093/nar/gku1308
10.1093/genetics/77.1.71
10.1038/nature11352
10.1016/j.semcdb.2015.10.017
10.1093/nar/gks1032
10.1038/nsmb.2801
10.1242/dev.02060
10.1016/j.devcel.2015.07.010
10.1093/nar/gkr954
10.1016/j.cell.2009.01.053
ContentType Journal Article
Copyright 2018. This work is published under http://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2018. This work is published under http://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID AAYXX
CITATION
3V.
7X7
7XB
88E
88I
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M2P
M7P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.7554/eLife.35478
DatabaseName CrossRef
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
Science Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni Edition)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Biological Science Collection
Health & Medical Collection (Alumni Edition)
Medical Database
Science Database
Biological Science Database
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Health & Medical Research Collection
Biological Science Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest Science Journals (Alumni Edition)
ProQuest Biological Science Collection
ProQuest Central Basic
ProQuest Science Journals
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database

CrossRef
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2050-084X
ExternalDocumentID oai_doaj_org_article_02c779641e7847d38284aedcaf454370
PMC6351104
10_7554_eLife_35478
GeographicLocations Australia
United States--US
GeographicLocations_xml – name: United States--US
– name: Australia
GrantInformation_xml – fundername: ;
– fundername: ;
  grantid: 0606575
– fundername: ;
  grantid: MOP-125894
– fundername: ;
  grantid: CAP- 783 262134
– fundername: ;
  grantid: HD078253
– fundername: ;
  grantid: DK075147
– fundername: ;
  grantid: MOP-274660
GroupedDBID 53G
5VS
7X7
88E
88I
8FE
8FH
8FI
8FJ
AAFWJ
AAKDD
AAYXX
ABUWG
ACGFO
ACGOD
ACPRK
ADBBV
ADRAZ
AENEX
AFKRA
AFPKN
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
CCPQU
CITATION
DIK
DWQXO
EMOBN
FYUFA
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
HMCUK
HYE
IAO
IEA
IHR
INH
INR
ISR
ITC
KQ8
LK8
M1P
M2P
M48
M7P
M~E
NQS
OK1
PGMZT
PHGZM
PHGZT
PIMPY
PQQKQ
PROAC
PSQYO
RHI
RNS
RPM
UKHRP
3V.
7XB
8FK
K9.
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQUKI
PRINS
Q9U
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c452t-89b7786608597c6c1fd4337812ddb99a2e2d8f6d4994250d74fccb5e52456ade3
IEDL.DBID 7X7
ISSN 2050-084X
IngestDate Wed Aug 27 01:10:25 EDT 2025
Thu Aug 21 13:42:12 EDT 2025
Fri Jul 11 10:41:13 EDT 2025
Fri Jul 25 09:14:05 EDT 2025
Thu Apr 24 23:07:23 EDT 2025
Tue Jul 01 03:59:45 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Language English
License http://creativecommons.org/publicdomain/zero/1.0
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c452t-89b7786608597c6c1fd4337812ddb99a2e2d8f6d4994250d74fccb5e52456ade3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Monash Institute of Pharmaceutical Sciences, Monash University, Victoria, Australia.
These authors contributed equally to this work.
ORCID 0000-0002-0889-0859
OpenAccessLink https://www.proquest.com/docview/2174215721?pq-origsite=%requestingapplication%
PMID 30575518
PQID 2174215721
PQPubID 2045579
ParticipantIDs doaj_primary_oai_doaj_org_article_02c779641e7847d38284aedcaf454370
pubmedcentral_primary_oai_pubmedcentral_nih_gov_6351104
proquest_miscellaneous_2159982459
proquest_journals_2174215721
crossref_citationtrail_10_7554_eLife_35478
crossref_primary_10_7554_eLife_35478
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2018-12-21
PublicationDateYYYYMMDD 2018-12-21
PublicationDate_xml – month: 12
  year: 2018
  text: 2018-12-21
  day: 21
PublicationDecade 2010
PublicationPlace Cambridge
PublicationPlace_xml – name: Cambridge
PublicationTitle eLife
PublicationYear 2018
Publisher eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
Publisher_xml – name: eLife Sciences Publications Ltd
– name: eLife Sciences Publications, Ltd
References Hammell (bib20) 2009; 136
de Albuquerque (bib15) 2015; 34
Shirayama (bib50) 2012; 150
Reinke (bib45) 2004; 131
Spracklin (bib53) 2017; 206
Laver (bib26) 2015; 16
Gu (bib19) 2009; 36
Yi (bib67) 2015; 11
Ikeda (bib22) 2012; 770
Ashe (bib3) 2012; 150
Boag (bib6) 2005; 132
Ray (bib42) 2009; 27
Yook (bib68) 2012; 40
Billi (bib5) 2014; 7
Ni (bib35) 2016; 9
Tu (bib59) 2015; 43
Tocchini (bib55) 2014; 10
Youngman (bib69) 2014; 5
Edwards (bib17) 2003; 17
Phillips (bib39) 2012; 26
Ray (bib44) 2017; 118
van Wolfswinkel (bib60) 2009; 139
Wedeles (bib63) 2013; 21
Audhya (bib4) 2005; 171
Boag (bib7) 2008; 182
Karp (bib23) 2012; 139
Schwamborn (bib46) 2009; 136
Langmead (bib25) 2009; 10
Brenner (bib9) 1974; 77
Phillips (bib40) 2014; 24
Neumüller (bib34) 2008; 454
Seth (bib48) 2013; 27
Cecere (bib11) 2014; 21
Duchaine (bib16) 2006; 124
Sengupta (bib47) 2012; 64
Tocchini (bib56) 2015; 47
Davis (bib14) 2017; 7
Wedeles (bib64) 2013; 27
Ortiz (bib36) 2014; 4
Weiser (bib66) 2017; 41
Hyenne (bib21) 2008; 321
McJunkin (bib31) 2017; 31
Buckley (bib10) 2012; 489
Spike (bib52) 2014; 198
Timmons (bib54) 1998; 395
Voronina (bib61) 2011; 3
Ray (bib43) 2013; 499
Bosher (bib8) 1999; 153
Tsukamoto (bib58) 2017; 206
Loedige (bib28) 2013; 41
Loedige (bib29) 2014; 28
Navarro (bib33) 2001; 128
Aoki (bib1) 2007; 26
Arnold (bib2) 2014; 42
Claycomb (bib12) 2009; 139
Kudryashova (bib24) 2005; 354
Phillips (bib41) 2015; 34
Paix (bib38) 2014; 198
Gerson-Gurwitz (bib18) 2016; 165
Pace (bib37) 1995; 4
She (bib49) 2009; 5
Trapnell (bib57) 2009; 25
Lewis (bib27) 2005; 120
Loedige (bib30) 2015; 13
Wedeles (bib65) 2014; 3
Conine (bib13) 2013; 155
Wang (bib62) 2008; 18
Nakamura (bib32) 2007; 12
Sonoda (bib51) 2001; 15
References_xml – volume: 5
  start-page: e1000624
  year: 2009
  ident: bib49
  article-title: Regulation of heterochromatin assembly on unpaired chromosomes during Caenorhabditis elegans meiosis by components of a small RNA-mediated pathway
  publication-title: PLoS Genetics
  doi: 10.1371/journal.pgen.1000624
– volume: 206
  start-page: 2007
  year: 2017
  ident: bib58
  article-title: LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans
  publication-title: Genetics
  doi: 10.1534/genetics.117.203174
– volume: 150
  start-page: 65
  year: 2012
  ident: bib50
  article-title: piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline
  publication-title: Cell
  doi: 10.1016/j.cell.2012.06.015
– volume: 31
  start-page: 422
  year: 2017
  ident: bib31
  article-title: A microRNA family exerts maternal control on sex determination in C. elegans
  publication-title: Genes & Development
  doi: 10.1101/gad.290155.116
– volume: 131
  start-page: 311
  year: 2004
  ident: bib45
  article-title: Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans
  publication-title: Development
  doi: 10.1242/dev.00914
– volume: 11
  start-page: e1005580
  year: 2015
  ident: bib67
  article-title: A Genetic Cascade of let-7-ncl-1-fib-1 Modulates Nucleolar Size and rRNA Pool in Caenorhabditis elegans
  publication-title: PLoS Genetics
  doi: 10.1371/journal.pgen.1005580
– volume: 27
  start-page: 664
  year: 2013
  ident: bib64
  article-title: Protection of germline gene expression by the C. elegans Argonaute CSR-1
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2013.11.016
– volume: 9
  start-page: 3
  year: 2016
  ident: bib35
  article-title: A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C. elegans
  publication-title: Epigenetics & Chromatin
  doi: 10.1186/s13072-016-0052-x
– volume: 206
  start-page: 1403
  year: 2017
  ident: bib53
  article-title: The RNAi Inheritance Machinery of Caenorhabditis elegans
  publication-title: Genetics
  doi: 10.1534/genetics.116.198812
– volume: 13
  start-page: 1206
  year: 2015
  ident: bib30
  article-title: The crystal structure of the nhl domain in complex with rna reveals the molecular basis of drosophila brain-tumor-mediated gene regulation
  publication-title: Cell Reports
  doi: 10.1016/j.celrep.2015.09.068
– volume: 165
  start-page: 396
  year: 2016
  ident: bib18
  article-title: A small rna-catalytic argonaute pathway tunes germline transcript levels to ensure embryonic divisions
  publication-title: Cell
  doi: 10.1016/j.cell.2016.02.040
– volume: 5
  start-page: 416
  year: 2014
  ident: bib69
  article-title: From early lessons to new frontiers: the worm as a treasure trove of small RNA biology
  publication-title: Frontiers in Genetics
  doi: 10.3389/fgene.2014.00416
– volume: 4
  start-page: 2411
  year: 1995
  ident: bib37
  article-title: How to measure and predict the molar absorption coefficient of a protein
  publication-title: Protein Science
  doi: 10.1002/pro.5560041120
– volume: 770
  start-page: 27
  year: 2012
  ident: bib22
  article-title: TRIM proteins as RING finger E3 ubiquitin ligases
  publication-title: Advances in Experimental Medicine and Biology
  doi: 10.1007/978-1-4614-5398-7_3
– volume: 136
  start-page: 913
  year: 2009
  ident: bib46
  article-title: The TRIM-NHL protein TRIM32 activates microRNAs and prevents self-renewal in mouse neural progenitors
  publication-title: Cell
  doi: 10.1016/j.cell.2008.12.024
– volume: 120
  start-page: 15
  year: 2005
  ident: bib27
  article-title: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
  publication-title: Cell
  doi: 10.1016/j.cell.2004.12.035
– volume: 321
  start-page: 368
  year: 2008
  ident: bib21
  article-title: C. elegans Brat homologs regulate PAR protein-dependent polarity and asymmetric cell division
  publication-title: Developmental Biology
  doi: 10.1016/j.ydbio.2008.06.037
– volume: 28
  start-page: 749
  year: 2014
  ident: bib29
  article-title: The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation
  publication-title: Genes & Development
  doi: 10.1101/gad.236513.113
– volume: 21
  start-page: 573
  year: 2013
  ident: bib63
  article-title: A multitasking Argonaute: exploring the many facets of C. elegans CSR-1
  publication-title: Chromosome Research
  doi: 10.1007/s10577-013-9383-7
– volume: 42
  start-page: 13353
  year: 2014
  ident: bib2
  article-title: Functional characterization of C. elegans Y-box-binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes
  publication-title: Nucleic Acids Research
  doi: 10.1093/nar/gku1077
– volume: 118
  start-page: 3
  year: 2017
  ident: bib44
  article-title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins
  publication-title: Methods
  doi: 10.1016/j.ymeth.2016.12.003
– volume: 64
  start-page: 586
  year: 2012
  ident: bib47
  article-title: Germ granules and the control of mRNA translation
  publication-title: IUBMB Life
  doi: 10.1002/iub.1039
– volume: 139
  start-page: 2177
  year: 2012
  ident: bib23
  article-title: Dauer larva quiescence alters the circuitry of microRNA pathways regulating cell fate progression in C. elegans
  publication-title: Development
  doi: 10.1242/dev.075986
– volume: 27
  start-page: 656
  year: 2013
  ident: bib48
  article-title: The C. elegans CSR-1 argonaute pathway counteracts epigenetic silencing to promote germline gene expression
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2013.11.014
– volume: 4
  start-page: 1765
  year: 2014
  ident: bib36
  article-title: A new dataset of spermatogenic vs. oogenic transcriptomes in the nematode Caenorhabditis elegans
  publication-title: G3: Genes|Genomes|Genetics
  doi: 10.1534/g3.114.012351
– volume: 7
  start-page: 3251
  year: 2017
  ident: bib14
  article-title: Exploring potential germline-associated roles of the trim-nhl protein nhl-2 through rnai screening
  publication-title: G3: Genes|Genomes|Genetics
  doi: 10.1534/g3.117.300166
– volume: 454
  start-page: 241
  year: 2008
  ident: bib34
  article-title: Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage
  publication-title: Nature
  doi: 10.1038/nature07014
– volume: 198
  start-page: 1347
  year: 2014
  ident: bib38
  article-title: Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans
  publication-title: Genetics
  doi: 10.1534/genetics.114.170423
– volume: 171
  start-page: 267
  year: 2005
  ident: bib4
  article-title: A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans
  publication-title: The Journal of Cell Biology
  doi: 10.1083/jcb.200506124
– volume: 27
  start-page: 667
  year: 2009
  ident: bib42
  article-title: Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins
  publication-title: Nature Biotechnology
  doi: 10.1038/nbt.1550
– volume: 139
  start-page: 135
  year: 2009
  ident: bib60
  article-title: CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs
  publication-title: Cell
  doi: 10.1016/j.cell.2009.09.012
– volume: 26
  start-page: 5007
  year: 2007
  ident: bib1
  article-title: In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans
  publication-title: The EMBO Journal
  doi: 10.1038/sj.emboj.7601910
– volume: 17
  start-page: 2508
  year: 2003
  ident: bib17
  article-title: Model of the brain tumor-Pumilio translation repressor complex
  publication-title: Genes & Development
  doi: 10.1101/gad.1119403
– volume: 12
  start-page: 997
  year: 2007
  ident: bib32
  article-title: Dicer-related drh-3 gene functions in germ-line development by maintenance of chromosomal integrity in Caenorhabditis elegans
  publication-title: Genes to Cells
  doi: 10.1111/j.1365-2443.2007.01111.x
– volume: 395
  start-page: 854
  year: 1998
  ident: bib54
  article-title: Specific interference by ingested dsRNA
  publication-title: Nature
  doi: 10.1038/27579
– volume: 3
  start-page: e28641
  year: 2014
  ident: bib65
  article-title: Silent no more: Endogenous small RNA pathways promote gene expression
  publication-title: Worm
  doi: 10.4161/worm.28641
– volume: 7
  start-page: 1
  year: 2014
  ident: bib5
  article-title: Endogenous RNAi pathways in C. elegans
  publication-title: WormBook
  doi: 10.1895/wormbook.1.170.1
– volume: 41
  start-page: 408
  year: 2017
  ident: bib66
  article-title: MORC-1 integrates nuclear rnai and transgenerational chromatin architecture to promote germline immortality
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2017.04.023
– volume: 36
  start-page: 231
  year: 2009
  ident: bib19
  article-title: Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2009.09.020
– volume: 499
  start-page: 172
  year: 2013
  ident: bib43
  article-title: A compendium of RNA-binding motifs for decoding gene regulation
  publication-title: Nature
  doi: 10.1038/nature12311
– volume: 155
  start-page: 1532
  year: 2013
  ident: bib13
  article-title: Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans
  publication-title: Cell
  doi: 10.1016/j.cell.2013.11.032
– volume: 24
  start-page: 839
  year: 2014
  ident: bib40
  article-title: MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation
  publication-title: Current Biology
  doi: 10.1016/j.cub.2014.02.060
– volume: 10
  start-page: e1004533
  year: 2014
  ident: bib55
  article-title: The TRIM-NHL protein LIN-41 controls the onset of developmental plasticity in Caenorhabditis elegans
  publication-title: PLoS Genetics
  doi: 10.1371/journal.pgen.1004533
– volume: 153
  start-page: 1245
  year: 1999
  ident: bib8
  article-title: RNA interference can target pre-mRNA: consequences for gene expression in a Caenorhabditis elegans operon
  publication-title: Genetics
  doi: 10.1093/genetics/153.3.1245
– volume: 182
  start-page: 543
  year: 2008
  ident: bib7
  article-title: Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis
  publication-title: The Journal of Cell Biology
  doi: 10.1083/jcb.200801183
– volume: 25
  start-page: 1105
  year: 2009
  ident: bib57
  article-title: TopHat: discovering splice junctions with RNA-Seq
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp120
– volume: 10
  start-page: R25
  year: 2009
  ident: bib25
  article-title: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
  publication-title: Genome Biology
  doi: 10.1186/gb-2009-10-3-r25
– volume: 26
  start-page: 1433
  year: 2012
  ident: bib39
  article-title: MUT-16 promotes formation of perinuclear mutator foci required for RNA silencing in the C. elegans germline
  publication-title: Genes & Development
  doi: 10.1101/gad.193904.112
– volume: 15
  start-page: 762
  year: 2001
  ident: bib51
  article-title: Drosophila Brain Tumor is a translational repressor
  publication-title: Genes & Development
  doi: 10.1101/gad.870801
– volume: 18
  start-page: 861
  year: 2008
  ident: bib62
  article-title: A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis
  publication-title: Current Biology
  doi: 10.1016/j.cub.2008.05.009
– volume: 124
  start-page: 343
  year: 2006
  ident: bib16
  article-title: Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways
  publication-title: Cell
  doi: 10.1016/j.cell.2005.11.036
– volume: 198
  start-page: 1535
  year: 2014
  ident: bib52
  article-title: The TRIM-NHL protein LIN-41 and the OMA RNA-binding proteins antagonistically control the prophase-to-metaphase transition and growth of Caenorhabditis elegans oocytes
  publication-title: Genetics
  doi: 10.1534/genetics.114.168831
– volume: 128
  start-page: 3221
  year: 2001
  ident: bib33
  article-title: cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans
  publication-title: Development
  doi: 10.1242/dev.128.17.3221
– volume: 139
  start-page: 123
  year: 2009
  ident: bib12
  article-title: The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation
  publication-title: Cell
  doi: 10.1016/j.cell.2009.09.014
– volume: 16
  start-page: 94
  year: 2015
  ident: bib26
  article-title: Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition
  publication-title: Genome Biology
  doi: 10.1186/s13059-015-0659-4
– volume: 3
  start-page: a002774
  year: 2011
  ident: bib61
  article-title: RNA granules in germ cells
  publication-title: Cold Spring Harbor Perspectives in Biology
  doi: 10.1101/cshperspect.a002774
– volume: 354
  start-page: 413
  year: 2005
  ident: bib24
  article-title: Trim32 is a ubiquitin ligase mutated in limb girdle muscular dystrophy type 2H that binds to skeletal muscle myosin and ubiquitinates actin
  publication-title: Journal of Molecular Biology
  doi: 10.1016/j.jmb.2005.09.068
– volume: 150
  start-page: 88
  year: 2012
  ident: bib3
  article-title: piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans
  publication-title: Cell
  doi: 10.1016/j.cell.2012.06.018
– volume: 34
  start-page: 457
  year: 2015
  ident: bib41
  article-title: piRNAs and piRNA-Dependent siRNAs protect conserved and essential c. elegans genes from misrouting into the RNAi pathway
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2015.07.009
– volume: 43
  start-page: 208
  year: 2015
  ident: bib59
  article-title: Comparative functional characterization of the CSR-1 22G-RNA pathway in Caenorhabditis nematodes
  publication-title: Nucleic Acids Research
  doi: 10.1093/nar/gku1308
– volume: 77
  start-page: 71
  year: 1974
  ident: bib9
  article-title: The genetics ofCaenorhabditis elegans
  publication-title: Genetics
  doi: 10.1093/genetics/77.1.71
– volume: 489
  start-page: 447
  year: 2012
  ident: bib10
  article-title: A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality
  publication-title: Nature
  doi: 10.1038/nature11352
– volume: 47
  start-page: 52
  year: 2015
  ident: bib56
  article-title: TRIM-NHL proteins in development and disease
  publication-title: Seminars in Cell & Developmental Biology
  doi: 10.1016/j.semcdb.2015.10.017
– volume: 41
  start-page: 518
  year: 2013
  ident: bib28
  article-title: The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
  publication-title: Nucleic Acids Research
  doi: 10.1093/nar/gks1032
– volume: 21
  start-page: 358
  year: 2014
  ident: bib11
  article-title: Global effects of the CSR-1 RNA interference pathway on the transcriptional landscape
  publication-title: Nature Structural & Molecular Biology
  doi: 10.1038/nsmb.2801
– volume: 132
  start-page: 4975
  year: 2005
  ident: bib6
  article-title: A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans
  publication-title: Development
  doi: 10.1242/dev.02060
– volume: 34
  start-page: 448
  year: 2015
  ident: bib15
  article-title: Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans
  publication-title: Developmental Cell
  doi: 10.1016/j.devcel.2015.07.010
– volume: 40
  start-page: D735
  year: 2012
  ident: bib68
  article-title: WormBase 2012: more genomes, more data, new website
  publication-title: Nucleic Acids Research
  doi: 10.1093/nar/gkr954
– volume: 136
  start-page: 926
  year: 2009
  ident: bib20
  article-title: nhl-2 Modulates microRNA activity in Caenorhabditis elegans
  publication-title: Cell
  doi: 10.1016/j.cell.2009.01.053
SSID ssj0000748819
Score 2.2659664
Snippet Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of...
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of...
SourceID doaj
pubmedcentral
proquest
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Enrichment Source
Index Database
SubjectTerms 22G RNAs
Biology
Biosynthesis
Caenorhabditis elegans
Chromatin
Chromosomes and Gene Expression
Gene expression
Genetics and Genomics
Genomes
Homeostasis
Meiosis
Proteins
Ribonucleic acid
RNA
RNA polymerase
RNA-binding protein
RNA-mediated interference
RNAi
small RNAs
TRIM-NHL
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA4iCF7EJ9YXETwJ0T6SJjmui7KK7kEUvNU0SbEgXXFXxH_vTFqXLQhevJV0KOnMJDMfmXxDyEkphOdZJpmOSwUARShWemeZtl5rV2XIUofVFuN89MhvnsTTQqsvrAlr6YFbxZ3HqZV4XTLxEjZSlwFC4Aa-ZioueCYDWoeYtwCmwh4swTET3V7IkxAyz_1tXfmzDOmreiEoMPX30st-ceRCtLlaJ2tdmkgH7fQ2yJJvNslK2zjya4s8g3Xpw_31HRuPbmngWqgbCs8spfWUGmonrO2kQ2EccjzaIG-xeaemcXQ6CTyt9H48qCm2JP40X1MUHJ5RbEMB0WubPF5dPgxHrOuVwCwX6YwpXSITXI58ZdLmNqkcmgDCt3Ol1ib1qVNV7gDgwCqNneSVtaXwAg8-jfPZDlluJo3fJVQrnbrKKJ2omHsljbBV7GCtZrkzpXUROf1RX2E7InHsZ_FaAKBAXRdB10XQdURO5sJvLX_G72IXaIe5CJJehwFwhaJzheIvV4jIwY8Vi24lTguEXJDWANCNyPH8NawhPBgxjZ98oIwA1Ama0BGRPev3JtR_09QvgY07x6PYmO_9xx_sk1VIyBSWy6TJAVmevX_4Q0h6ZuVR8O9v3Ef-OQ
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Na9wwEB3SlEIvoekHdZoWFXIqaOsvWdKppKFhW5I9hCzk5sqS3BiCnexuaPffd0b2LnUIvRl5jM2MxvMGSe8BHFVC-DzLJNdxpbBBEYpX3lmurdfa1Rmx1NFui1kxnec_rsTVDmzEOAcHLh9t7UhPar64mfy5W3_BhEf8OpFYDT_7s6b2k4yYqZ7AUyxJkjL0fMD54ZcscZ4muj-f9_CZUUUKxP0jtDneK_lP8Tl9AXsDamTHfZj3Yce3L-FZryO5fgU_Mdjs8uL7OZ9Nz1igXmhahtc8Zc2SGWY73gvrMBxHyMdaojE2C2Zax5ZdoG1lF7PjhpFC8W-zXpLhyYSRKgUWs9cwP_12eTLlg3QCt7lIV1zpiojhCqIvk7awSe0oIljNnau0NqlPnaoLh_0OJm3sZF5bWwkvaB3UOJ-9gd22a_1bYFrp1NVG6UTFuVfSCFvHDlM3K5yprIvg08Z9pR14xUne4qbE_oJ8XQZfl8HXERxtjW97Oo3Hzb5SHLYmxIEdBrrFr3JIqTJOraSDtImXWGJdhr1jbjBQps5Fnsk4gsNNFMvNvCqpA0OUg31vBB-3tzGlaJ3EtL67JxuBTSh6QkcgR9EffdD4TttcB3LuglZm4_zg_y9_B88ReSnaF5Mmh7C7Wtz794huVtWHMHP_ArWY-R4
  priority: 102
  providerName: Scholars Portal
Title The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
URI https://www.proquest.com/docview/2174215721
https://www.proquest.com/docview/2159982459
https://pubmed.ncbi.nlm.nih.gov/PMC6351104
https://doaj.org/article/02c779641e7847d38284aedcaf454370
Volume 7
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fa9swED62lsFexn4yb13QoE8DtY4tWdLTaEtLNtowQgt582RJ3gzD7pKU0f9-d7KTzTD2Yox1YHOn83cnnb4DOKykDCLPFTdppTFBkZpXwTtuXDDG1zmx1FG1xbyY3YjPS7kcFtzWQ1nl9p8Yf9S-c7RGfkyhM8ITJiwfb39y6hpFu6tDC42HsE_UZVTSpZZqt8aC8KgR8fpjeQqB8zhcNnU4yonEagREka9_FGSOSyT_wpyLp_BkCBbZSW_dZ_AgtM_hUd8-8v4FfEUbs-vFpys-n12yyLjQtAzvecaaNbPMdbzvp8PwOUZ6rCX2YrtitvVs3UW2VraYnzSMGhP_svdrEjw7YtSMAjHsJdxcnF-fzfjQMYE7IbMN16YiPriCWMuUK9y09mQIBHHvK2NsFjKv68JjmoO-mnolaucqGSRtf1of8lew13ZteA3MaJP52moz1akIWlnp6tSjx-aFt5XzCXzYqq90A504dbX4UWJaQbouo67LqOsEDnfCtz2Lxr_FTskOOxGivo4PutW3cvCkMs2covOz06AQWX2OKaOwaChbCylylSZwsLViOfjjuvwzexJ4vxtGT6LtEduG7o5kJOaeqAmTgBpZf_RB45G2-R45uQvakE3Fm_-__C08xoBLUzlMNj2Avc3qLrzDoGZTTeLMncD-6fn8y2ISlwbweiX0b3zo-gE
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3fa9RAEB7qFdEX8SeNVl2hvghpc8km2X0QaWvLnb0GOa7Qt3Szu9GAJPVypdw_5d_oTH6cBsS3vh3JkAszs_vNZHe_D2AvC0PLgyB2pZcJbFBC4WbWaFdqK6XJA2Kpo90WSTS54F8uw8st-NWfhaFtlf2c2EzUptL0jfyASmeEJ2xYPl3_dEk1ilZXewmNNi3O7PoWW7b64_Qzxve975-eLI4nbqcq4Goe-itXyIw40yJi9op1pMe5oZdFoDMmk1L51jcijwy2ApjPnol5rnUW2pCWCJWxAT73HmzzAFuZEWwfnSRf55uvOgjIAjG2PQgYI1Qf2FmR2_2AaLMG0NcoBAzK2uGmzL9Q7vQxPOrKU3bY5tMT2LLlU7jfClaun8EVZhVbzKfnbjKZsYbjoSgZ_nZ9VtRMMV25rYIPw-tYW7KS-JLVkqnSsLpq-GHZPDksGEkh36p1TYbH-4zkLxA1n8PFnXjzBYzKqrQ7wKSQvsmVkGPhcStiFercMzhHBJFRmTYOfOjdl-qOwJx0NH6k2MiQr9PG12njawf2NsbXLW_Hv82OKA4bEyLbbi5Uy29pN3ZTz9cxndgd2xix3ATYpHKFgVI5D3kQew7s9lFMuxmgTv_kqwPvNrdx7NKCjCptdUM2IXa76AnpQDyI_uCFhnfK4nvDAh7RErDHX_7_z9_Cg8nifJbOpsnZK3iI5Z6gzTj-eBdGq-WNfY0l1Sp70-Uxg6u7Hjq_Ae6sND4
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VIhAXxFMYCixSuSBt49d6vQeESkuU0BChqpVyc9e7a7CE7JKkqvLX-HXM-BGwhLj1Ftkjx5rHzox39vsA9nMhXBxFkis_T7FBESnPnTVcGaeULSJCqaNpi3kyOY8_L8RiB371Z2ForLJfE5uF2taGvpGPqHTG9IQNy6joxiK-Ho8_XP7kxCBFO609nUbrIiduc43t2-r99Bht_TYMx5_Ojia8YxjgJhbhmqcqJ_y0hFC-pElMUFh6cUx61uZK6dCFNi0Si20B-rZvZVwYkwsnaLtQWxfhc2_BbRmJgGJMLuT2-w6m5hSzbXskUGLSHrlZWbiDiAC0Bkmw4QoYFLjD8cy_8t34AdzvClV22HrWQ9hx1SO401JXbh7DBfoXOzudfuHzyYw1aA9lxfA3D1m5YpqZmrdcPgyvY5XJKkJO1kumK8tWdYMUy07nhyUjUuRrvVmR4NEBIyIMzJ9P4PxGdPkUdqu6cs-AqVSFttCpClI_dqnUwhS-xdUiSqzOjfXgXa--zHRQ5sSo8SPDloZ0nTW6zhpde7C_Fb5sETz-LfaR7LAVIdjt5kK9_JZ1UZz5oZF0djdwErO6jbBdjTUaShexiCPpe7DXWzHr1oJV9sdzPXizvY1RTFszunL1FckI7HtRE8oDObD-4IWGd6rye4MHntBmsB8___-fv4a7GDDZbDo_eQH3sO5LaSonDPZgd728ci-xtlrnrxonZnBx01HzG-BeNw4
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+TRIM-NHL+protein+NHL-2+is+a+co-factor+in+the+nuclear+and+somatic+RNAi+pathways+in+C.+elegans&rft.jtitle=eLife&rft.au=Davis%2C+Gregory+M&rft.au=Tu+Shikui&rft.au=Anderson+Joshua+WT&rft.au=Colson%2C+Rhys+N&rft.date=2018-12-21&rft.pub=eLife+Sciences+Publications+Ltd&rft.eissn=2050-084X&rft.volume=7&rft_id=info:doi/10.7554%2FeLife.35478&rft.externalDBID=HAS_PDF_LINK
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2050-084X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2050-084X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2050-084X&client=summon