Immunostimulatory DNA sequences inhibit respiratory syncytial viral load, airway inflammation, and mucus secretion

• Published in: Journal of allergy and clinical immunology Vol. 108; no. 5; pp. 697 - 702
• Main Authors: Cho, Jae Youn, Miller, Marina, Baek, Kwang Je, Castaneda, Diego, Nayar, Jyothi, Roman, Mark, Raz, Eyal, Broide, David H.
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.11.2001; Elsevier
• Subjects: Adjuvants, Immunologic - therapeutic use; Allergic diseases; Animals; Asthma - drug therapy; Asthma - metabolism; Asthma - virology; Biological and medical sciences; Bronchial Hyperreactivity - diagnosis; Bronchitis - drug therapy; Bronchitis - virology; Bronchoconstrictor Agents; Cell Line; Chronic obstructive pulmonary disease, asthma; DNA - therapeutic use; Female; IFN-γ, asthma; Immunopathology; Immunostimulatory DNA sequence; Interferon-gamma - biosynthesis; Interferons - pharmacology; Interleukin-13 - biosynthesis; Lung - metabolism; Lung - virology; Medical sciences; methacholine; Methacholine Chloride; Mice; Mice, Inbred BALB C; Mucus - secretion; Pneumology; Respiratory and ent allergic diseases; Respiratory syncytial virus; Respiratory Syncytial Virus Infections - drug therapy; Respiratory Syncytial Virus Infections - metabolism; Respiratory Syncytial Virus Infections - virology; Respiratory Syncytial Viruses - growth & development; Respiratory Syncytial Viruses - isolation & purification; Viral Load; Virus Replication - drug effects; PAS; RSV; ISS; Penh; Immunostimulatory DNA sequence; M-ODN; respiratory syncytial virus; BAL; NK; IFN-γ, asthma; Animal model; Hyperreactivity; Spumavirinae; Prevention; Respiratory tract; Complication; Obstructive pulmonary disease; Mechanism of action; Aminoacid sequence; Immunostimulation; Respiratory disease; Rodentia; Retroviridae; Inflammation; Asthma; Infection; Virus; Vertebrata; Mammalia; Human syncytial virus; Mouse; Animal; DNA; Viral disease; Gamma interferon

Background: Immunostimulatory DNA sequences (ISS) activate the innate immune system to generate antiviral cytokines, such as IFN-γ. Objective: This study investigated whether ISS could reduce viral load, mucus secretion, airway inflammation, and airway hyperreactivity to methacholine in a mouse mode...