High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system

• Published in: New biotechnology Vol. 31; no. 3; pp. 214 - 220
• Main Authors: Hou, Jeff Jia Cheng, Hughes, Ben S., Smede, Matthew, Leung, Kar Man, Levine, Kara, Rigby, Susan, Gray, Peter P., Munro, Trent P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.05.2014
• Subjects: Animals; Antibodies, Monoclonal - metabolism; Base Sequence; Batch Cell Culture Techniques; CHO Cells; Chromatin - metabolism; Clone Cells; Cricetinae; Cricetulus; Genetic Vectors - metabolism; Guinea Pigs; High-Throughput Screening Assays - methods; Humans; Immunoglobulin G - metabolism; Promoter Regions, Genetic - genetics; Transfection

•UCOE-containing cells show advantage in recombinant antibody expression.•Cell line generation involving the use of semi-solid cloning and ClonePix FL.•High throughput technology has a crucial role in clonal isolation.•Example of a clear, simple and streamlined path for biopharmaceutical development...