Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA

How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein...

Full description

Saved in:
Bibliographic Details
Published inCurrent biology Vol. 27; no. 14; pp. 2101 - 2111.e5
Main Authors Ramat, Anne, Hannaford, Matthew, Januschke, Jens
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 24.07.2017
Elsevier
Cell Press
Subjects
Animals
asymmetric cell division
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila melanogaster - physiology
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Larva - genetics
Larva - physiology
Life Sciences
Male
Mitosis
mRNA localization
nanobody
Neural Stem Cells - metabolism
neuroblasts
polarity
RNA, Messenger - genetics
RNA, Messenger - metabolism
mRNA localization
asymmetric cell division
nanobody
Drosophila
neuroblasts
polarity
Online AccessGet full text

Cover

Abstract How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA. [Display omitted] •Nanobody technology and the MS2 system can be used to alter mRNA localization•miranda mRNA localizes in two distinct pools in Drosophila neuroblasts in mitosis•Asymmetric Miranda localization requires an mRNA-dependent maintenance step Ramat et al. combine the MS2 system and nanobody expression to alter the subcellular localization of mRNA. Shifting basally localized miranda mRNA in Drosophila neuroblasts to the apical pole resulted in Miranda protein localization defects. Miranda protein and cognate mRNA interaction positively feeds back on asymmetric Miranda localization.
AbstractList How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA. [Display omitted] •Nanobody technology and the MS2 system can be used to alter mRNA localization•miranda mRNA localizes in two distinct pools in Drosophila neuroblasts in mitosis•Asymmetric Miranda localization requires an mRNA-dependent maintenance step Ramat et al. combine the MS2 system and nanobody expression to alter the subcellular localization of mRNA. Shifting basally localized miranda mRNA in Drosophila neuroblasts to the apical pole resulted in Miranda protein localization defects. Miranda protein and cognate mRNA interaction positively feeds back on asymmetric Miranda localization.
How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA. • Nanobody technology and the MS2 system can be used to alter mRNA localization • miranda mRNA localizes in two distinct pools in Drosophila neuroblasts in mitosis • Asymmetric Miranda localization requires an mRNA-dependent maintenance step Ramat et al. combine the MS2 system and nanobody expression to alter the subcellular localization of mRNA. Shifting basally localized miranda mRNA in Drosophila neuroblasts to the apical pole resulted in Miranda protein localization defects. Miranda protein and cognate mRNA interaction positively feeds back on asymmetric Miranda localization.
How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA.
Author Ramat, Anne
Hannaford, Matthew
Januschke, Jens
AuthorAffiliation 1 Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dow Street, DD5 1EH Dundee, UK
AuthorAffiliation_xml – name: 1 Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dow Street, DD5 1EH Dundee, UK
Author_xml – sequence: 1
  givenname: Anne
  surname: Ramat
  fullname: Ramat, Anne
  organization: Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dow Street, DD5 1EH Dundee, UK
– sequence: 2
  givenname: Matthew
  surname: Hannaford
  fullname: Hannaford, Matthew
  organization: Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dow Street, DD5 1EH Dundee, UK
– sequence: 3
  givenname: Jens
  surname: Januschke
  fullname: Januschke, Jens
  email: j.januschke@dundee.ac.uk
  organization: Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dow Street, DD5 1EH Dundee, UK
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28690114$$D View this record in MEDLINE/PubMed
https://hal.science/hal-04736818$$DView record in HAL
BookMark eNp9kVFrFDEUhYNU7Lb6A3yRvPow400yk2QQhGWttrCtIPocMpmkk2U2WTLZFf31zbpa1Aef7uXknEO43wU6CzFYhF4SqAkQ_mZTm31fUyCiBl4X5QlaECm6CpqmPUML6DhUnaT0HF3M8waAUNnxZ-icSt4BIc0Cjbfah2yDDsbi6PCtTzoMGq-j0ZP_obOPAfuA36c4x93oJ43v7D7FftJznvFNOMTpYI9Ltkmbn_ZvPo84jxav4n3Q2eLt57vlc_TU6Wm2L37NS_T1w9WX1XW1_vTxZrVcV6Zpaa6Yc9w1EgZrenBCCGcIbfuOAYiBMCmFYZYx4xylVPBB0oY3hHSi40YLB-wSvTv17vb91g7Ghpz0pHbJb3X6rqL26u-X4Ed1Hw-qbSmXjJWC16eC8Z_Y9XKtjho0gnFJ5IEULzl5TTnPnKx7DBBQR0RqowoidUSkgKuilMyrPz_4mPjNpBjengy2nOngbVKz8bbwGXyyJqsh-v_UPwD_LqTA
CitedBy_id crossref_primary_10_1021_acs_biochem_9b00813
crossref_primary_10_1083_jcb_202112097
crossref_primary_10_3389_fgene_2019_00135
crossref_primary_10_1016_j_ceb_2019_07_018
crossref_primary_10_1083_jcb_201807037
crossref_primary_10_7554_eLife_29939
crossref_primary_10_3390_ijms221910267
crossref_primary_10_1016_j_ceb_2019_06_001
crossref_primary_10_1242_dev_167650
crossref_primary_10_1242_dev_170589
crossref_primary_10_1007_s11427_020_1702_x
crossref_primary_10_1016_j_jmb_2017_09_013
crossref_primary_10_1074_jbc_REV120_012960
crossref_primary_10_7554_eLife_97902
crossref_primary_10_3390_antib8010016
crossref_primary_10_15252_embj_2018100373
crossref_primary_10_1016_j_devcel_2023_04_006
crossref_primary_10_1016_j_bbagrm_2018_08_004
Cites_doi 10.1016/S0076-6879(04)89022-0
10.1016/S1097-2765(00)80143-4
10.1242/dev.014977
10.1038/ng1632
10.1038/35861
10.1038/embor.2011.178
10.1016/j.cell.2009.01.044
10.1242/jcs.01525
10.1101/gad.190413.112
10.1038/990128
10.1016/j.cub.2004.10.022
10.1016/0092-8674(91)90463-9
10.1242/dev.02456
10.1016/S0092-8674(00)80505-X
10.1016/S0092-8674(00)80504-8
10.1101/gad.12.12.1837
10.1534/genetics.106.065961
10.1242/dev.01919
10.1242/dev.121.9.2737
10.1038/nrm3918
10.1038/37641
10.1016/j.ceb.2009.11.011
10.1038/35050550
10.1038/nature01486
10.1016/S0960-9822(98)00016-5
10.1101/gad.11.4.423
10.1038/35046094
10.1242/jcs.112.10.1541
10.1242/dev.100933
10.1038/358387a0
10.1083/jcb.200512137
10.1016/S0960-9822(99)80070-0
10.1016/0092-8674(91)90138-O
10.1016/j.yexcr.2007.02.018
10.1038/nmeth.1253
10.1016/S0896-6273(00)80701-1
10.1038/377624a0
10.1016/j.cub.2009.03.056
10.1016/j.cell.2008.02.007
10.1083/jcb.200306079
10.1038/ncb2671
10.1016/j.devcel.2015.09.016
10.1016/j.cell.2014.03.005
10.1083/jcb.150.6.1361
10.1126/science.1176488
10.1242/jcs.020024
10.1101/gad.12.12.1847
10.1016/S0960-9822(06)00370-8
10.1016/j.biochi.2010.11.004
10.1016/j.jinsphys.2011.03.014
10.1038/nmeth.2019
10.1016/j.cell.2008.07.049
10.1242/dev.077081
10.1242/dev.125.20.4089
10.1101/gad.531009
10.1038/nature16443
10.1038/nrm1643
10.1093/nar/gkt702
10.1016/S0092-8674(00)81753-5
10.1016/j.cell.2007.08.003
ContentType Journal Article
Copyright 2017 The Author(s)
Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Distributed under a Creative Commons Attribution 4.0 International License
2017 The Author(s) 2017
Copyright_xml – notice: 2017 The Author(s)
– notice: Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
– notice: Distributed under a Creative Commons Attribution 4.0 International License
– notice: 2017 The Author(s) 2017
DBID 6I.
AAFTH
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
1XC
5PM
DOI 10.1016/j.cub.2017.06.016
DatabaseName ScienceDirect Open Access Titles
Elsevier:ScienceDirect:Open Access
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Hyper Article en Ligne (HAL)
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
DatabaseTitleList

MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1879-0445
EndPage 2111.e5
ExternalDocumentID oai_HAL_hal_04736818v1
10_1016_j_cub_2017_06_016
28690114
S0960982217307182
Genre Journal Article
GrantInformation_xml – fundername: Wellcome Trust
GroupedDBID ---
--K
-DZ
-~X
0R~
1RT
1~5
2WC
4.4
457
4G.
53G
5GY
62-
6I.
6J9
7-5
AACTN
AAEDW
AAFTH
AAFWJ
AAIAV
AAKRW
AALRI
AAUCE
AAVLU
AAXJY
AAXUO
ABJNI
ABMAC
ABMWF
ABVKL
ACGFO
ACGFS
ADBBV
ADEZE
ADJPV
AEFWE
AENEX
AEXQZ
AFTJW
AGHSJ
AGKMS
AGUBO
AHHHB
AITUG
ALKID
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
AZFZN
BAWUL
CS3
DIK
DU5
E3Z
EBS
EJD
F5P
FCP
FDB
FIRID
IHE
IXB
J1W
JIG
LX5
M3Z
M41
NCXOZ
O-L
O9-
OK1
P2P
RCE
RIG
ROL
RPZ
SCP
SDG
SES
SSZ
TR2
WQ6
ZA5
0SF
AAEDT
AAMRU
ADVLN
AKAPO
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
29F
5VS
AAIKJ
AAQFI
AAQXK
AAYXX
ACRPL
ADMUD
ADNMO
AGHFR
ASPBG
AVWKF
CAG
CITATION
COF
FEDTE
FGOYB
G-2
HVGLF
HZ~
OZT
R2-
SEW
UHS
XIH
XPP
Y6R
ZGI
1XC
5PM
ID FETCH-LOGICAL-c452t-3ff6f480decb0f777fc125b93007d13887c3e33cff22276d82464119796ca7f03
IEDL.DBID ABVKL
ISSN 0960-9822
IngestDate Tue Sep 17 20:51:57 EDT 2024
Wed Oct 16 06:46:44 EDT 2024
Fri Dec 06 06:32:28 EST 2024
Wed Oct 16 00:58:26 EDT 2024
Fri Feb 23 02:28:30 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 14
Keywords mRNA localization
asymmetric cell division
nanobody
Drosophila
neuroblasts
polarity
Language English
License This is an open access article under the CC BY license.
Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c452t-3ff6f480decb0f777fc125b93007d13887c3e33cff22276d82464119796ca7f03
Notes Lead Contact
OpenAccessLink https://www.sciencedirect.com/science/article/pii/S0960982217307182
PMID 28690114
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_5526833
hal_primary_oai_HAL_hal_04736818v1
crossref_primary_10_1016_j_cub_2017_06_016
pubmed_primary_28690114
elsevier_sciencedirect_doi_10_1016_j_cub_2017_06_016
PublicationCentury 2000
PublicationDate 2017-07-24
PublicationDateYYYYMMDD 2017-07-24
PublicationDate_xml – month: 07
  year: 2017
  text: 2017-07-24
  day: 24
PublicationDecade 2010
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Current biology
PublicationTitleAlternate Curr Biol
PublicationYear 2017
Publisher Elsevier Ltd
Elsevier
Cell Press
Publisher_xml – name: Elsevier Ltd
– name: Elsevier
– name: Cell Press
References Buszczak, Paterno, Lighthouse, Bachman, Planck, Owen, Skora, Nystul, Ohlstein, Allen (bib57) 2007; 175
Mach, Lehmann (bib33) 1997; 11
Bailey, Prehoda (bib49) 2015; 35
Ponton, Chapuis, Pernice, Sword, Simpson (bib58) 2011; 57
Kloc, Bilinski, Dougherty (bib11) 2007; 313
Rongo, Gavis, Lehmann (bib53) 1995; 121
Baena-Lopez, Alexandre, Mitchell, Pasakarnis, Vincent (bib59) 2013; 140
Li, Yang, Wasser, Cai, Chia (bib35) 1997; 90
Martin, Ephrussi (bib6) 2009; 136
Broadus, Fuerstenberg, Doe (bib32) 1998; 391
Shen, Knoblich, Chan, Jiang, Jan, Jan (bib47) 1998; 12
Lim, Kumari, Gilligan, Quach, Mathavan, Sampath (bib9) 2012; 139
Doe, Chu-LaGraff, Wright, Scott (bib23) 1991; 65
Lee, Luo (bib56) 1999; 22
Hughes, Bullock, Ish-Horowicz (bib27) 2004; 14
Schuldt, Adams, Davidson, Micklem, Haseloff, St Johnston, Brand (bib30) 1998; 12
Rodriguez, Shenoy, Singer, Condeelis (bib60) 2006; 175
Wirtz-Peitz, Nishimura, Knoblich (bib62) 2008; 135
Petronczki, Knoblich (bib19) 2001; 3
Lu, Rothenberg, Jan, Jan (bib22) 1998; 95
Katz, Wells, Park, Wu, Shenoy, Singer (bib41) 2012; 26
Matsuzaki, Ohshiro, Ikeshima-Kataoka, Izumi (bib50) 1998; 125
Dienstbier, Boehl, Li, Bullock (bib37) 2009; 23
Peng, Manning, Albertson, Doe (bib51) 2000; 408
St Johnston (bib3) 2005; 6
Lécuyer, Yoshida, Parthasarathy, Alm, Babak, Cerovina, Hughes, Tomancak, Krause (bib5) 2007; 131
Holt, Bullock (bib1) 2009; 326
Broadus, Doe (bib36) 1997; 7
Knoblich, Jan, Jan (bib28) 1999; 9
Derivery, Seum, Daeden, Loubéry, Holtzer, Jülicher, González-Gaitán (bib43) 2015; 528
Kuchinke, Grawe, Knust (bib29) 1998; 8
Yu (bib31) 2004; 389
JayaNandanan, Gavis, Riechmann, Leptin (bib54) 2011; 12
Tio, Zavortink, Yang, Chia (bib52) 1999; 112
Caussinus, Gonzalez (bib40) 2005; 37
Shen, Jan, Jan (bib39) 1997; 90
Jenny, Hachet, Závorszky, Cyrklaff, Weston, Johnston, Erdélyi, Ephrussi (bib13) 2006; 133
Buxbaum, Haimovich, Singer (bib4) 2015; 16
Doe (bib15) 2008; 135
Zhang, Huang, Bao, Cong, Wang, Chai, Xi, Ge, Somers, Yang (bib25) 2016; 143
Schindelin, Arganda-Carreras, Frise, Kaynig, Longair, Pietzsch, Preibisch, Rueden, Saalfeld, Schmid (bib61) 2012; 9
Rolls, Albertson, Shih, Lee, Doe (bib18) 2003; 163
Groth, Fish, Nusse, Calos (bib45) 2004; 166
Knoblich, Jan, Jan (bib21) 1995; 377
Kloc, Wilk, Vargas, Shirato, Bilinski, Etkin (bib10) 2005; 132
Wodarz, Ramrath, Kuchinke, Knust (bib16) 1999; 402
Meignin, Davis (bib2) 2010; 22
Bertrand, Chartrand, Schaefer, Shenoy, Singer, Long (bib42) 1998; 2
Ulveling, Francastel, Hubé (bib8) 2011; 93
Wodarz, Ramrath, Grimm, Knust (bib17) 2000; 150
Ikeshima-Kataoka, Skeath, Nabeshima, Doe, Matsuzaki (bib20) 1997; 390
Laver, Li, Ancevicius, Westwood, Smibert, Morris, Lipshitz (bib38) 2013; 41
St Johnston, Beuchle, Nüsslein-Volhard (bib34) 1991; 66
Erben, Waldhuber, Langer, Fetka, Jansen, Petritsch (bib26) 2008; 121
Januschke, Reina, Llamazares, Bertran, Rossi, Roig, Gonzalez (bib46) 2013; 15
Ephrussi, Lehmann (bib12) 1992; 358
Knoblich (bib14) 2008; 132
Albertson, Chabu, Sheehan, Doe (bib55) 2004; 117
Raj, van den Bogaard, Rifkin, van Oudenaarden, Tyagi (bib44) 2008; 5
Atwood, Prehoda (bib24) 2009; 19
Betschinger, Mechtler, Knoblich (bib48) 2003; 422
Jung, Gkogkas, Sonenberg, Holt (bib7) 2014; 157
16142234 - Nat Genet. 2005 Oct;37(10):1125-9
22743772 - Nat Methods. 2012 Jun 28;9(7):676-82
1712672 - Cell. 1991 Jul 12;66(1):51-63
9486649 - Nature. 1998 Feb 19;391(6669):792-5
18356248 - Development. 2008 May;135(9):1575-87
18398000 - J Cell Sci. 2008 May 1;121(Pt 9):1403-14
24154526 - Development. 2013 Dec;140(23):4818-25
11146625 - Nat Cell Biol. 2001 Jan;3(1):43-9
23354166 - Nat Cell Biol. 2013 Mar;15(3):241-8
9267025 - Cell. 1997 Aug 8;90(3):449-58
15126397 - Genetics. 2004 Apr;166(4):1775-82
19375318 - Curr Biol. 2009 May 12;19(9):723-9
16000384 - Development. 2005 Aug;132(15):3445-57
7566172 - Nature. 1995 Oct 19;377(6550):624-7
20022233 - Curr Opin Cell Biol. 2010 Feb;22(1):112-9
21435341 - J Insect Physiol. 2011 Jun;57(6):840-50
16835436 - Development. 2006 Aug;133(15):2827-33
19965463 - Science. 2009 Nov 27;326(5957):1212-6
24679524 - Cell. 2014 Mar 27;157(1):26-40
10197526 - Neuron. 1999 Mar;22(3):451-61
23945942 - Nucleic Acids Res. 2013 Nov;41(20):9438-60
22721777 - Development. 2012 Aug;139(16):2903-15
11117748 - Nature. 2000 Nov 30;408(6812):596-600
12629552 - Nature. 2003 Mar 20;422(6929):326-30
21921935 - EMBO Rep. 2011 Oct 28;12(11):1167-74
18806792 - Nat Methods. 2008 Oct;5(10):877-9
15852043 - Nat Rev Mol Cell Biol. 2005 May;6(5):363-75
10591216 - Nature. 1999 Dec 2;402(6761):544-7
22948660 - Genes Dev. 2012 Sep 1;26(17):1885-90
9637685 - Genes Dev. 1998 Jun 15;12(12):1837-46
18295577 - Cell. 2008 Feb 22;132(4):583-97
9042857 - Genes Dev. 1997 Feb 15;11(4):423-35
19515976 - Genes Dev. 2009 Jul 1;23(13):1546-58
9790529 - Cell. 1998 Oct 16;95(2):225-35
17194782 - Genetics. 2007 Mar;175(3):1505-31
26481050 - Dev Cell. 2015 Oct 26;35(2):199-210
9735369 - Development. 1998 Oct;125(20):4089-98
10021388 - Curr Biol. 1999 Feb 11;9(3):155-8
15313577 - Methods Enzymol. 2004;389:364-82
15536119 - J Cell Sci. 2004 Dec 1;117(Pt 25):6061-70
25549890 - Nat Rev Mol Cell Biol. 2015 Feb;16(2):95-109
10995441 - J Cell Biol. 2000 Sep 18;150(6):1361-74
26659188 - Nature. 2015 Dec 10;528(7581):280-5
10212148 - J Cell Sci. 1999 May;112 ( Pt 10):1541-51
17376434 - Exp Cell Res. 2007 May 1;313(8):1639-51
1673362 - Cell. 1991 May 3;65(3):451-64
21111023 - Biochimie. 2011 Apr;93(4):633-44
7555702 - Development. 1995 Sep;121(9):2737-46
9403694 - Nature. 1997 Dec 11;390(6660):625-9
9637686 - Genes Dev. 1998 Jun 15;12(12):1847-57
17923096 - Cell. 2007 Oct 5;131(1):174-87
1641021 - Nature. 1992 Jul 30;358(6385):387-92
15530398 - Curr Biol. 2004 Nov 9;14(21):1950-6
19239891 - Cell. 2009 Feb 20;136(4):719-30
26586222 - Development. 2016 Jan 1;143(1):35-44
9382803 - Curr Biol. 1997 Nov 1;7(11):827-35
17030983 - J Cell Biol. 2006 Oct 9;175(1):67-76
18854163 - Cell. 2008 Oct 3;135(1):161-73
14657233 - J Cell Biol. 2003 Dec 8;163(5):1089-98
9809065 - Mol Cell. 1998 Oct;2(4):437-45
9889099 - Curr Biol. 1998 Dec 17-31;8(25):1357-65
9267024 - Cell. 1997 Aug 8;90(3):437-47
Peng (10.1016/j.cub.2017.06.016_bib51) 2000; 408
Petronczki (10.1016/j.cub.2017.06.016_bib19) 2001; 3
St Johnston (10.1016/j.cub.2017.06.016_bib34) 1991; 66
Betschinger (10.1016/j.cub.2017.06.016_bib48) 2003; 422
Kuchinke (10.1016/j.cub.2017.06.016_bib29) 1998; 8
Bailey (10.1016/j.cub.2017.06.016_bib49) 2015; 35
Holt (10.1016/j.cub.2017.06.016_bib1) 2009; 326
Broadus (10.1016/j.cub.2017.06.016_bib36) 1997; 7
Ulveling (10.1016/j.cub.2017.06.016_bib8) 2011; 93
Doe (10.1016/j.cub.2017.06.016_bib15) 2008; 135
Mach (10.1016/j.cub.2017.06.016_bib33) 1997; 11
Rodriguez (10.1016/j.cub.2017.06.016_bib60) 2006; 175
Jenny (10.1016/j.cub.2017.06.016_bib13) 2006; 133
Knoblich (10.1016/j.cub.2017.06.016_bib28) 1999; 9
Caussinus (10.1016/j.cub.2017.06.016_bib40) 2005; 37
Lim (10.1016/j.cub.2017.06.016_bib9) 2012; 139
Hughes (10.1016/j.cub.2017.06.016_bib27) 2004; 14
Rongo (10.1016/j.cub.2017.06.016_bib53) 1995; 121
Wirtz-Peitz (10.1016/j.cub.2017.06.016_bib62) 2008; 135
Knoblich (10.1016/j.cub.2017.06.016_bib14) 2008; 132
Lu (10.1016/j.cub.2017.06.016_bib22) 1998; 95
Wodarz (10.1016/j.cub.2017.06.016_bib16) 1999; 402
Ponton (10.1016/j.cub.2017.06.016_bib58) 2011; 57
Martin (10.1016/j.cub.2017.06.016_bib6) 2009; 136
Kloc (10.1016/j.cub.2017.06.016_bib10) 2005; 132
Kloc (10.1016/j.cub.2017.06.016_bib11) 2007; 313
Shen (10.1016/j.cub.2017.06.016_bib39) 1997; 90
Groth (10.1016/j.cub.2017.06.016_bib45) 2004; 166
Ephrussi (10.1016/j.cub.2017.06.016_bib12) 1992; 358
Buszczak (10.1016/j.cub.2017.06.016_bib57) 2007; 175
Katz (10.1016/j.cub.2017.06.016_bib41) 2012; 26
Januschke (10.1016/j.cub.2017.06.016_bib46) 2013; 15
Meignin (10.1016/j.cub.2017.06.016_bib2) 2010; 22
Broadus (10.1016/j.cub.2017.06.016_bib32) 1998; 391
Lécuyer (10.1016/j.cub.2017.06.016_bib5) 2007; 131
Tio (10.1016/j.cub.2017.06.016_bib52) 1999; 112
Atwood (10.1016/j.cub.2017.06.016_bib24) 2009; 19
Li (10.1016/j.cub.2017.06.016_bib35) 1997; 90
Baena-Lopez (10.1016/j.cub.2017.06.016_bib59) 2013; 140
Rolls (10.1016/j.cub.2017.06.016_bib18) 2003; 163
Wodarz (10.1016/j.cub.2017.06.016_bib17) 2000; 150
Matsuzaki (10.1016/j.cub.2017.06.016_bib50) 1998; 125
Schindelin (10.1016/j.cub.2017.06.016_bib61) 2012; 9
Buxbaum (10.1016/j.cub.2017.06.016_bib4) 2015; 16
Shen (10.1016/j.cub.2017.06.016_bib47) 1998; 12
St Johnston (10.1016/j.cub.2017.06.016_bib3) 2005; 6
Derivery (10.1016/j.cub.2017.06.016_bib43) 2015; 528
Albertson (10.1016/j.cub.2017.06.016_bib55) 2004; 117
Raj (10.1016/j.cub.2017.06.016_bib44) 2008; 5
Yu (10.1016/j.cub.2017.06.016_bib31) 2004; 389
Schuldt (10.1016/j.cub.2017.06.016_bib30) 1998; 12
Bertrand (10.1016/j.cub.2017.06.016_bib42) 1998; 2
Lee (10.1016/j.cub.2017.06.016_bib56) 1999; 22
Ikeshima-Kataoka (10.1016/j.cub.2017.06.016_bib20) 1997; 390
Doe (10.1016/j.cub.2017.06.016_bib23) 1991; 65
Laver (10.1016/j.cub.2017.06.016_bib38) 2013; 41
Knoblich (10.1016/j.cub.2017.06.016_bib21) 1995; 377
Zhang (10.1016/j.cub.2017.06.016_bib25) 2016; 143
Dienstbier (10.1016/j.cub.2017.06.016_bib37) 2009; 23
JayaNandanan (10.1016/j.cub.2017.06.016_bib54) 2011; 12
Erben (10.1016/j.cub.2017.06.016_bib26) 2008; 121
Jung (10.1016/j.cub.2017.06.016_bib7) 2014; 157
References_xml – volume: 140
  start-page: 4818
  year: 2013
  end-page: 4825
  ident: bib59
  article-title: Accelerated homologous recombination and subsequent genome modification in Drosophila
  publication-title: Development
  contributor:
    fullname: Vincent
– volume: 143
  start-page: 35
  year: 2016
  end-page: 44
  ident: bib25
  article-title: Phosphotyrosyl phosphatase activator facilitates localization of Miranda through dephosphorylation in dividing neuroblasts
  publication-title: Development
  contributor:
    fullname: Yang
– volume: 16
  start-page: 95
  year: 2015
  end-page: 109
  ident: bib4
  article-title: In the right place at the right time: visualizing and understanding mRNA localization
  publication-title: Nat. Rev. Mol. Cell Biol.
  contributor:
    fullname: Singer
– volume: 93
  start-page: 633
  year: 2011
  end-page: 644
  ident: bib8
  article-title: When one is better than two: RNA with dual functions
  publication-title: Biochimie
  contributor:
    fullname: Hubé
– volume: 125
  start-page: 4089
  year: 1998
  end-page: 4098
  ident: bib50
  article-title: miranda localizes staufen and prospero asymmetrically in mitotic neuroblasts and epithelial cells in early Drosophila embryogenesis
  publication-title: Development
  contributor:
    fullname: Izumi
– volume: 131
  start-page: 174
  year: 2007
  end-page: 187
  ident: bib5
  article-title: Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function
  publication-title: Cell
  contributor:
    fullname: Krause
– volume: 11
  start-page: 423
  year: 1997
  end-page: 435
  ident: bib33
  article-title: An Egalitarian-BicaudalD complex is essential for oocyte specification and axis determination in Drosophila
  publication-title: Genes Dev.
  contributor:
    fullname: Lehmann
– volume: 37
  start-page: 1125
  year: 2005
  end-page: 1129
  ident: bib40
  article-title: Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster
  publication-title: Nat. Genet.
  contributor:
    fullname: Gonzalez
– volume: 12
  start-page: 1847
  year: 1998
  end-page: 1857
  ident: bib30
  article-title: Miranda mediates asymmetric protein and RNA localization in the developing nervous system
  publication-title: Genes Dev.
  contributor:
    fullname: Brand
– volume: 377
  start-page: 624
  year: 1995
  end-page: 627
  ident: bib21
  article-title: Asymmetric segregation of Numb and Prospero during cell division
  publication-title: Nature
  contributor:
    fullname: Jan
– volume: 135
  start-page: 161
  year: 2008
  end-page: 173
  ident: bib62
  article-title: Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization
  publication-title: Cell
  contributor:
    fullname: Knoblich
– volume: 112
  start-page: 1541
  year: 1999
  end-page: 1551
  ident: bib52
  article-title: A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions
  publication-title: J. Cell Sci.
  contributor:
    fullname: Chia
– volume: 19
  start-page: 723
  year: 2009
  end-page: 729
  ident: bib24
  article-title: aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division
  publication-title: Curr. Biol.
  contributor:
    fullname: Prehoda
– volume: 8
  start-page: 1357
  year: 1998
  end-page: 1365
  ident: bib29
  article-title: Control of spindle orientation in Drosophila by the Par-3-related PDZ-domain protein Bazooka
  publication-title: Curr. Biol.
  contributor:
    fullname: Knust
– volume: 12
  start-page: 1837
  year: 1998
  end-page: 1846
  ident: bib47
  article-title: Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila
  publication-title: Genes Dev.
  contributor:
    fullname: Jan
– volume: 139
  start-page: 2903
  year: 2012
  end-page: 2915
  ident: bib9
  article-title: Dorsal activity of maternal squint is mediated by a non-coding function of the RNA
  publication-title: Development
  contributor:
    fullname: Sampath
– volume: 35
  start-page: 199
  year: 2015
  end-page: 210
  ident: bib49
  article-title: Establishment of par-polarized cortical domains via phosphoregulated membrane motifs
  publication-title: Dev. Cell
  contributor:
    fullname: Prehoda
– volume: 117
  start-page: 6061
  year: 2004
  end-page: 6070
  ident: bib55
  article-title: Scribble protein domain mapping reveals a multistep localization mechanism and domains necessary for establishing cortical polarity
  publication-title: J. Cell Sci.
  contributor:
    fullname: Doe
– volume: 121
  start-page: 2737
  year: 1995
  end-page: 2746
  ident: bib53
  article-title: Localization of oskar RNA regulates oskar translation and requires Oskar protein
  publication-title: Development
  contributor:
    fullname: Lehmann
– volume: 175
  start-page: 67
  year: 2006
  end-page: 76
  ident: bib60
  article-title: Visualization of mRNA translation in living cells
  publication-title: J. Cell Biol.
  contributor:
    fullname: Condeelis
– volume: 9
  start-page: 155
  year: 1999
  end-page: 158
  ident: bib28
  article-title: Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization
  publication-title: Curr. Biol.
  contributor:
    fullname: Jan
– volume: 163
  start-page: 1089
  year: 2003
  end-page: 1098
  ident: bib18
  article-title: Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
  publication-title: J. Cell Biol.
  contributor:
    fullname: Doe
– volume: 390
  start-page: 625
  year: 1997
  end-page: 629
  ident: bib20
  article-title: Miranda directs Prospero to a daughter cell during Drosophila asymmetric divisions
  publication-title: Nature
  contributor:
    fullname: Matsuzaki
– volume: 22
  start-page: 112
  year: 2010
  end-page: 119
  ident: bib2
  article-title: Transmitting the message: intracellular mRNA localization
  publication-title: Curr. Opin. Cell Biol.
  contributor:
    fullname: Davis
– volume: 22
  start-page: 451
  year: 1999
  end-page: 461
  ident: bib56
  article-title: Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
  publication-title: Neuron
  contributor:
    fullname: Luo
– volume: 5
  start-page: 877
  year: 2008
  end-page: 879
  ident: bib44
  article-title: Imaging individual mRNA molecules using multiple singly labeled probes
  publication-title: Nat. Methods
  contributor:
    fullname: Tyagi
– volume: 402
  start-page: 544
  year: 1999
  end-page: 547
  ident: bib16
  article-title: Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
  publication-title: Nature
  contributor:
    fullname: Knust
– volume: 150
  start-page: 1361
  year: 2000
  end-page: 1374
  ident: bib17
  article-title: Drosophila atypical protein kinase C associates with Bazooka and controls polarity of epithelia and neuroblasts
  publication-title: J. Cell Biol.
  contributor:
    fullname: Knust
– volume: 90
  start-page: 449
  year: 1997
  end-page: 458
  ident: bib39
  article-title: Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila
  publication-title: Cell
  contributor:
    fullname: Jan
– volume: 57
  start-page: 840
  year: 2011
  end-page: 850
  ident: bib58
  article-title: Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster
  publication-title: J. Insect Physiol.
  contributor:
    fullname: Simpson
– volume: 9
  start-page: 676
  year: 2012
  end-page: 682
  ident: bib61
  article-title: Fiji: an open-source platform for biological-image analysis
  publication-title: Nat. Methods
  contributor:
    fullname: Schmid
– volume: 23
  start-page: 1546
  year: 2009
  end-page: 1558
  ident: bib37
  article-title: Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor
  publication-title: Genes Dev.
  contributor:
    fullname: Bullock
– volume: 389
  start-page: 364
  year: 2004
  end-page: 382
  ident: bib31
  article-title: Analysis of the roles of Pins and heterotrimeric G proteins in asymmetric division of Drosophila neuroblasts
  publication-title: Methods Enzymol.
  contributor:
    fullname: Yu
– volume: 326
  start-page: 1212
  year: 2009
  end-page: 1216
  ident: bib1
  article-title: Subcellular mRNA localization in animal cells and why it matters
  publication-title: Science
  contributor:
    fullname: Bullock
– volume: 358
  start-page: 387
  year: 1992
  end-page: 392
  ident: bib12
  article-title: Induction of germ cell formation by oskar
  publication-title: Nature
  contributor:
    fullname: Lehmann
– volume: 12
  start-page: 1167
  year: 2011
  end-page: 1174
  ident: bib54
  article-title: A genetic in vivo system to detect asymmetrically distributed RNA
  publication-title: EMBO Rep.
  contributor:
    fullname: Leptin
– volume: 95
  start-page: 225
  year: 1998
  end-page: 235
  ident: bib22
  article-title: Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors
  publication-title: Cell
  contributor:
    fullname: Jan
– volume: 422
  start-page: 326
  year: 2003
  end-page: 330
  ident: bib48
  article-title: The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
  publication-title: Nature
  contributor:
    fullname: Knoblich
– volume: 121
  start-page: 1403
  year: 2008
  end-page: 1414
  ident: bib26
  article-title: Asymmetric localization of the adaptor protein Miranda in neuroblasts is achieved by diffusion and sequential interaction of Myosin II and VI
  publication-title: J. Cell Sci.
  contributor:
    fullname: Petritsch
– volume: 2
  start-page: 437
  year: 1998
  end-page: 445
  ident: bib42
  article-title: Localization of ASH1 mRNA particles in living yeast
  publication-title: Mol. Cell
  contributor:
    fullname: Long
– volume: 528
  start-page: 280
  year: 2015
  end-page: 285
  ident: bib43
  article-title: Polarized endosome dynamics by spindle asymmetry during asymmetric cell division
  publication-title: Nature
  contributor:
    fullname: González-Gaitán
– volume: 14
  start-page: 1950
  year: 2004
  end-page: 1956
  ident: bib27
  article-title: Inscuteable mRNA localization is dynein-dependent and regulates apicobasal polarity and spindle length in Drosophila neuroblasts
  publication-title: Curr. Biol.
  contributor:
    fullname: Ish-Horowicz
– volume: 132
  start-page: 583
  year: 2008
  end-page: 597
  ident: bib14
  article-title: Mechanisms of asymmetric stem cell division
  publication-title: Cell
  contributor:
    fullname: Knoblich
– volume: 408
  start-page: 596
  year: 2000
  end-page: 600
  ident: bib51
  article-title: The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
  publication-title: Nature
  contributor:
    fullname: Doe
– volume: 15
  start-page: 241
  year: 2013
  end-page: 248
  ident: bib46
  article-title: Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts
  publication-title: Nat. Cell Biol.
  contributor:
    fullname: Gonzalez
– volume: 6
  start-page: 363
  year: 2005
  end-page: 375
  ident: bib3
  article-title: Moving messages: the intracellular localization of mRNAs
  publication-title: Nat. Rev. Mol. Cell Biol.
  contributor:
    fullname: St Johnston
– volume: 66
  start-page: 51
  year: 1991
  end-page: 63
  ident: bib34
  article-title: Staufen, a gene required to localize maternal RNAs in the Drosophila egg
  publication-title: Cell
  contributor:
    fullname: Nüsslein-Volhard
– volume: 41
  start-page: 9438
  year: 2013
  end-page: 9460
  ident: bib38
  article-title: Genome-wide analysis of Staufen-associated mRNAs identifies secondary structures that confer target specificity
  publication-title: Nucleic Acids Res.
  contributor:
    fullname: Lipshitz
– volume: 65
  start-page: 451
  year: 1991
  end-page: 464
  ident: bib23
  article-title: The prospero gene specifies cell fates in the Drosophila central nervous system
  publication-title: Cell
  contributor:
    fullname: Scott
– volume: 391
  start-page: 792
  year: 1998
  end-page: 795
  ident: bib32
  article-title: Staufen-dependent localization of prospero mRNA contributes to neuroblast daughter-cell fate
  publication-title: Nature
  contributor:
    fullname: Doe
– volume: 166
  start-page: 1775
  year: 2004
  end-page: 1782
  ident: bib45
  article-title: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
  publication-title: Genetics
  contributor:
    fullname: Calos
– volume: 313
  start-page: 1639
  year: 2007
  end-page: 1651
  ident: bib11
  article-title: Organization of cytokeratin cytoskeleton and germ plasm in the vegetal cortex of Xenopus laevis oocytes depends on coding and non-coding RNAs: three-dimensional and ultrastructural analysis
  publication-title: Exp. Cell Res.
  contributor:
    fullname: Dougherty
– volume: 3
  start-page: 43
  year: 2001
  end-page: 49
  ident: bib19
  article-title: DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
  publication-title: Nat. Cell Biol.
  contributor:
    fullname: Knoblich
– volume: 157
  start-page: 26
  year: 2014
  end-page: 40
  ident: bib7
  article-title: Remote control of gene function by local translation
  publication-title: Cell
  contributor:
    fullname: Holt
– volume: 136
  start-page: 719
  year: 2009
  end-page: 730
  ident: bib6
  article-title: mRNA localization: gene expression in the spatial dimension
  publication-title: Cell
  contributor:
    fullname: Ephrussi
– volume: 7
  start-page: 827
  year: 1997
  end-page: 835
  ident: bib36
  article-title: Extrinsic cues, intrinsic cues and microfilaments regulate asymmetric protein localization in Drosophila neuroblasts
  publication-title: Curr. Biol.
  contributor:
    fullname: Doe
– volume: 26
  start-page: 1885
  year: 2012
  end-page: 1890
  ident: bib41
  article-title: β-actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration
  publication-title: Genes Dev.
  contributor:
    fullname: Singer
– volume: 90
  start-page: 437
  year: 1997
  end-page: 447
  ident: bib35
  article-title: Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions
  publication-title: Cell
  contributor:
    fullname: Chia
– volume: 133
  start-page: 2827
  year: 2006
  end-page: 2833
  ident: bib13
  article-title: A translation-independent role of oskar RNA in early Drosophila oogenesis
  publication-title: Development
  contributor:
    fullname: Ephrussi
– volume: 132
  start-page: 3445
  year: 2005
  end-page: 3457
  ident: bib10
  article-title: Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes
  publication-title: Development
  contributor:
    fullname: Etkin
– volume: 135
  start-page: 1575
  year: 2008
  end-page: 1587
  ident: bib15
  article-title: Neural stem cells: balancing self-renewal with differentiation
  publication-title: Development
  contributor:
    fullname: Doe
– volume: 175
  start-page: 1505
  year: 2007
  end-page: 1531
  ident: bib57
  article-title: The carnegie protein trap library: a versatile tool for Drosophila developmental studies
  publication-title: Genetics
  contributor:
    fullname: Allen
– volume: 389
  start-page: 364
  year: 2004
  ident: 10.1016/j.cub.2017.06.016_bib31
  article-title: Analysis of the roles of Pins and heterotrimeric G proteins in asymmetric division of Drosophila neuroblasts
  publication-title: Methods Enzymol.
  doi: 10.1016/S0076-6879(04)89022-0
  contributor:
    fullname: Yu
– volume: 2
  start-page: 437
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib42
  article-title: Localization of ASH1 mRNA particles in living yeast
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(00)80143-4
  contributor:
    fullname: Bertrand
– volume: 135
  start-page: 1575
  year: 2008
  ident: 10.1016/j.cub.2017.06.016_bib15
  article-title: Neural stem cells: balancing self-renewal with differentiation
  publication-title: Development
  doi: 10.1242/dev.014977
  contributor:
    fullname: Doe
– volume: 37
  start-page: 1125
  year: 2005
  ident: 10.1016/j.cub.2017.06.016_bib40
  article-title: Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster
  publication-title: Nat. Genet.
  doi: 10.1038/ng1632
  contributor:
    fullname: Caussinus
– volume: 391
  start-page: 792
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib32
  article-title: Staufen-dependent localization of prospero mRNA contributes to neuroblast daughter-cell fate
  publication-title: Nature
  doi: 10.1038/35861
  contributor:
    fullname: Broadus
– volume: 12
  start-page: 1167
  year: 2011
  ident: 10.1016/j.cub.2017.06.016_bib54
  article-title: A genetic in vivo system to detect asymmetrically distributed RNA
  publication-title: EMBO Rep.
  doi: 10.1038/embor.2011.178
  contributor:
    fullname: JayaNandanan
– volume: 136
  start-page: 719
  year: 2009
  ident: 10.1016/j.cub.2017.06.016_bib6
  article-title: mRNA localization: gene expression in the spatial dimension
  publication-title: Cell
  doi: 10.1016/j.cell.2009.01.044
  contributor:
    fullname: Martin
– volume: 117
  start-page: 6061
  year: 2004
  ident: 10.1016/j.cub.2017.06.016_bib55
  article-title: Scribble protein domain mapping reveals a multistep localization mechanism and domains necessary for establishing cortical polarity
  publication-title: J. Cell Sci.
  doi: 10.1242/jcs.01525
  contributor:
    fullname: Albertson
– volume: 26
  start-page: 1885
  year: 2012
  ident: 10.1016/j.cub.2017.06.016_bib41
  article-title: β-actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration
  publication-title: Genes Dev.
  doi: 10.1101/gad.190413.112
  contributor:
    fullname: Katz
– volume: 402
  start-page: 544
  year: 1999
  ident: 10.1016/j.cub.2017.06.016_bib16
  article-title: Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
  publication-title: Nature
  doi: 10.1038/990128
  contributor:
    fullname: Wodarz
– volume: 14
  start-page: 1950
  year: 2004
  ident: 10.1016/j.cub.2017.06.016_bib27
  article-title: Inscuteable mRNA localization is dynein-dependent and regulates apicobasal polarity and spindle length in Drosophila neuroblasts
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2004.10.022
  contributor:
    fullname: Hughes
– volume: 65
  start-page: 451
  year: 1991
  ident: 10.1016/j.cub.2017.06.016_bib23
  article-title: The prospero gene specifies cell fates in the Drosophila central nervous system
  publication-title: Cell
  doi: 10.1016/0092-8674(91)90463-9
  contributor:
    fullname: Doe
– volume: 133
  start-page: 2827
  year: 2006
  ident: 10.1016/j.cub.2017.06.016_bib13
  article-title: A translation-independent role of oskar RNA in early Drosophila oogenesis
  publication-title: Development
  doi: 10.1242/dev.02456
  contributor:
    fullname: Jenny
– volume: 90
  start-page: 449
  year: 1997
  ident: 10.1016/j.cub.2017.06.016_bib39
  article-title: Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)80505-X
  contributor:
    fullname: Shen
– volume: 90
  start-page: 437
  year: 1997
  ident: 10.1016/j.cub.2017.06.016_bib35
  article-title: Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)80504-8
  contributor:
    fullname: Li
– volume: 12
  start-page: 1837
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib47
  article-title: Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila
  publication-title: Genes Dev.
  doi: 10.1101/gad.12.12.1837
  contributor:
    fullname: Shen
– volume: 175
  start-page: 1505
  year: 2007
  ident: 10.1016/j.cub.2017.06.016_bib57
  article-title: The carnegie protein trap library: a versatile tool for Drosophila developmental studies
  publication-title: Genetics
  doi: 10.1534/genetics.106.065961
  contributor:
    fullname: Buszczak
– volume: 132
  start-page: 3445
  year: 2005
  ident: 10.1016/j.cub.2017.06.016_bib10
  article-title: Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes
  publication-title: Development
  doi: 10.1242/dev.01919
  contributor:
    fullname: Kloc
– volume: 121
  start-page: 2737
  year: 1995
  ident: 10.1016/j.cub.2017.06.016_bib53
  article-title: Localization of oskar RNA regulates oskar translation and requires Oskar protein
  publication-title: Development
  doi: 10.1242/dev.121.9.2737
  contributor:
    fullname: Rongo
– volume: 16
  start-page: 95
  year: 2015
  ident: 10.1016/j.cub.2017.06.016_bib4
  article-title: In the right place at the right time: visualizing and understanding mRNA localization
  publication-title: Nat. Rev. Mol. Cell Biol.
  doi: 10.1038/nrm3918
  contributor:
    fullname: Buxbaum
– volume: 390
  start-page: 625
  year: 1997
  ident: 10.1016/j.cub.2017.06.016_bib20
  article-title: Miranda directs Prospero to a daughter cell during Drosophila asymmetric divisions
  publication-title: Nature
  doi: 10.1038/37641
  contributor:
    fullname: Ikeshima-Kataoka
– volume: 22
  start-page: 112
  year: 2010
  ident: 10.1016/j.cub.2017.06.016_bib2
  article-title: Transmitting the message: intracellular mRNA localization
  publication-title: Curr. Opin. Cell Biol.
  doi: 10.1016/j.ceb.2009.11.011
  contributor:
    fullname: Meignin
– volume: 3
  start-page: 43
  year: 2001
  ident: 10.1016/j.cub.2017.06.016_bib19
  article-title: DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
  publication-title: Nat. Cell Biol.
  doi: 10.1038/35050550
  contributor:
    fullname: Petronczki
– volume: 422
  start-page: 326
  year: 2003
  ident: 10.1016/j.cub.2017.06.016_bib48
  article-title: The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
  publication-title: Nature
  doi: 10.1038/nature01486
  contributor:
    fullname: Betschinger
– volume: 8
  start-page: 1357
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib29
  article-title: Control of spindle orientation in Drosophila by the Par-3-related PDZ-domain protein Bazooka
  publication-title: Curr. Biol.
  doi: 10.1016/S0960-9822(98)00016-5
  contributor:
    fullname: Kuchinke
– volume: 11
  start-page: 423
  year: 1997
  ident: 10.1016/j.cub.2017.06.016_bib33
  article-title: An Egalitarian-BicaudalD complex is essential for oocyte specification and axis determination in Drosophila
  publication-title: Genes Dev.
  doi: 10.1101/gad.11.4.423
  contributor:
    fullname: Mach
– volume: 143
  start-page: 35
  year: 2016
  ident: 10.1016/j.cub.2017.06.016_bib25
  article-title: Phosphotyrosyl phosphatase activator facilitates localization of Miranda through dephosphorylation in dividing neuroblasts
  publication-title: Development
  contributor:
    fullname: Zhang
– volume: 408
  start-page: 596
  year: 2000
  ident: 10.1016/j.cub.2017.06.016_bib51
  article-title: The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts
  publication-title: Nature
  doi: 10.1038/35046094
  contributor:
    fullname: Peng
– volume: 112
  start-page: 1541
  year: 1999
  ident: 10.1016/j.cub.2017.06.016_bib52
  article-title: A functional analysis of inscuteable and its roles during Drosophila asymmetric cell divisions
  publication-title: J. Cell Sci.
  doi: 10.1242/jcs.112.10.1541
  contributor:
    fullname: Tio
– volume: 140
  start-page: 4818
  year: 2013
  ident: 10.1016/j.cub.2017.06.016_bib59
  article-title: Accelerated homologous recombination and subsequent genome modification in Drosophila
  publication-title: Development
  doi: 10.1242/dev.100933
  contributor:
    fullname: Baena-Lopez
– volume: 358
  start-page: 387
  year: 1992
  ident: 10.1016/j.cub.2017.06.016_bib12
  article-title: Induction of germ cell formation by oskar
  publication-title: Nature
  doi: 10.1038/358387a0
  contributor:
    fullname: Ephrussi
– volume: 175
  start-page: 67
  year: 2006
  ident: 10.1016/j.cub.2017.06.016_bib60
  article-title: Visualization of mRNA translation in living cells
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200512137
  contributor:
    fullname: Rodriguez
– volume: 9
  start-page: 155
  year: 1999
  ident: 10.1016/j.cub.2017.06.016_bib28
  article-title: Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization
  publication-title: Curr. Biol.
  doi: 10.1016/S0960-9822(99)80070-0
  contributor:
    fullname: Knoblich
– volume: 66
  start-page: 51
  year: 1991
  ident: 10.1016/j.cub.2017.06.016_bib34
  article-title: Staufen, a gene required to localize maternal RNAs in the Drosophila egg
  publication-title: Cell
  doi: 10.1016/0092-8674(91)90138-O
  contributor:
    fullname: St Johnston
– volume: 313
  start-page: 1639
  year: 2007
  ident: 10.1016/j.cub.2017.06.016_bib11
  article-title: Organization of cytokeratin cytoskeleton and germ plasm in the vegetal cortex of Xenopus laevis oocytes depends on coding and non-coding RNAs: three-dimensional and ultrastructural analysis
  publication-title: Exp. Cell Res.
  doi: 10.1016/j.yexcr.2007.02.018
  contributor:
    fullname: Kloc
– volume: 5
  start-page: 877
  year: 2008
  ident: 10.1016/j.cub.2017.06.016_bib44
  article-title: Imaging individual mRNA molecules using multiple singly labeled probes
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1253
  contributor:
    fullname: Raj
– volume: 22
  start-page: 451
  year: 1999
  ident: 10.1016/j.cub.2017.06.016_bib56
  article-title: Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
  publication-title: Neuron
  doi: 10.1016/S0896-6273(00)80701-1
  contributor:
    fullname: Lee
– volume: 377
  start-page: 624
  year: 1995
  ident: 10.1016/j.cub.2017.06.016_bib21
  article-title: Asymmetric segregation of Numb and Prospero during cell division
  publication-title: Nature
  doi: 10.1038/377624a0
  contributor:
    fullname: Knoblich
– volume: 19
  start-page: 723
  year: 2009
  ident: 10.1016/j.cub.2017.06.016_bib24
  article-title: aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2009.03.056
  contributor:
    fullname: Atwood
– volume: 132
  start-page: 583
  year: 2008
  ident: 10.1016/j.cub.2017.06.016_bib14
  article-title: Mechanisms of asymmetric stem cell division
  publication-title: Cell
  doi: 10.1016/j.cell.2008.02.007
  contributor:
    fullname: Knoblich
– volume: 163
  start-page: 1089
  year: 2003
  ident: 10.1016/j.cub.2017.06.016_bib18
  article-title: Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200306079
  contributor:
    fullname: Rolls
– volume: 15
  start-page: 241
  year: 2013
  ident: 10.1016/j.cub.2017.06.016_bib46
  article-title: Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb2671
  contributor:
    fullname: Januschke
– volume: 35
  start-page: 199
  year: 2015
  ident: 10.1016/j.cub.2017.06.016_bib49
  article-title: Establishment of par-polarized cortical domains via phosphoregulated membrane motifs
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2015.09.016
  contributor:
    fullname: Bailey
– volume: 157
  start-page: 26
  year: 2014
  ident: 10.1016/j.cub.2017.06.016_bib7
  article-title: Remote control of gene function by local translation
  publication-title: Cell
  doi: 10.1016/j.cell.2014.03.005
  contributor:
    fullname: Jung
– volume: 150
  start-page: 1361
  year: 2000
  ident: 10.1016/j.cub.2017.06.016_bib17
  article-title: Drosophila atypical protein kinase C associates with Bazooka and controls polarity of epithelia and neuroblasts
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.150.6.1361
  contributor:
    fullname: Wodarz
– volume: 326
  start-page: 1212
  year: 2009
  ident: 10.1016/j.cub.2017.06.016_bib1
  article-title: Subcellular mRNA localization in animal cells and why it matters
  publication-title: Science
  doi: 10.1126/science.1176488
  contributor:
    fullname: Holt
– volume: 121
  start-page: 1403
  year: 2008
  ident: 10.1016/j.cub.2017.06.016_bib26
  article-title: Asymmetric localization of the adaptor protein Miranda in neuroblasts is achieved by diffusion and sequential interaction of Myosin II and VI
  publication-title: J. Cell Sci.
  doi: 10.1242/jcs.020024
  contributor:
    fullname: Erben
– volume: 12
  start-page: 1847
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib30
  article-title: Miranda mediates asymmetric protein and RNA localization in the developing nervous system
  publication-title: Genes Dev.
  doi: 10.1101/gad.12.12.1847
  contributor:
    fullname: Schuldt
– volume: 7
  start-page: 827
  year: 1997
  ident: 10.1016/j.cub.2017.06.016_bib36
  article-title: Extrinsic cues, intrinsic cues and microfilaments regulate asymmetric protein localization in Drosophila neuroblasts
  publication-title: Curr. Biol.
  doi: 10.1016/S0960-9822(06)00370-8
  contributor:
    fullname: Broadus
– volume: 93
  start-page: 633
  year: 2011
  ident: 10.1016/j.cub.2017.06.016_bib8
  article-title: When one is better than two: RNA with dual functions
  publication-title: Biochimie
  doi: 10.1016/j.biochi.2010.11.004
  contributor:
    fullname: Ulveling
– volume: 57
  start-page: 840
  year: 2011
  ident: 10.1016/j.cub.2017.06.016_bib58
  article-title: Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster
  publication-title: J. Insect Physiol.
  doi: 10.1016/j.jinsphys.2011.03.014
  contributor:
    fullname: Ponton
– volume: 9
  start-page: 676
  year: 2012
  ident: 10.1016/j.cub.2017.06.016_bib61
  article-title: Fiji: an open-source platform for biological-image analysis
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2019
  contributor:
    fullname: Schindelin
– volume: 135
  start-page: 161
  year: 2008
  ident: 10.1016/j.cub.2017.06.016_bib62
  article-title: Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization
  publication-title: Cell
  doi: 10.1016/j.cell.2008.07.049
  contributor:
    fullname: Wirtz-Peitz
– volume: 139
  start-page: 2903
  year: 2012
  ident: 10.1016/j.cub.2017.06.016_bib9
  article-title: Dorsal activity of maternal squint is mediated by a non-coding function of the RNA
  publication-title: Development
  doi: 10.1242/dev.077081
  contributor:
    fullname: Lim
– volume: 166
  start-page: 1775
  year: 2004
  ident: 10.1016/j.cub.2017.06.016_bib45
  article-title: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
  publication-title: Genetics
  contributor:
    fullname: Groth
– volume: 125
  start-page: 4089
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib50
  article-title: miranda localizes staufen and prospero asymmetrically in mitotic neuroblasts and epithelial cells in early Drosophila embryogenesis
  publication-title: Development
  doi: 10.1242/dev.125.20.4089
  contributor:
    fullname: Matsuzaki
– volume: 23
  start-page: 1546
  year: 2009
  ident: 10.1016/j.cub.2017.06.016_bib37
  article-title: Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor
  publication-title: Genes Dev.
  doi: 10.1101/gad.531009
  contributor:
    fullname: Dienstbier
– volume: 528
  start-page: 280
  year: 2015
  ident: 10.1016/j.cub.2017.06.016_bib43
  article-title: Polarized endosome dynamics by spindle asymmetry during asymmetric cell division
  publication-title: Nature
  doi: 10.1038/nature16443
  contributor:
    fullname: Derivery
– volume: 6
  start-page: 363
  year: 2005
  ident: 10.1016/j.cub.2017.06.016_bib3
  article-title: Moving messages: the intracellular localization of mRNAs
  publication-title: Nat. Rev. Mol. Cell Biol.
  doi: 10.1038/nrm1643
  contributor:
    fullname: St Johnston
– volume: 41
  start-page: 9438
  year: 2013
  ident: 10.1016/j.cub.2017.06.016_bib38
  article-title: Genome-wide analysis of Staufen-associated mRNAs identifies secondary structures that confer target specificity
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkt702
  contributor:
    fullname: Laver
– volume: 95
  start-page: 225
  year: 1998
  ident: 10.1016/j.cub.2017.06.016_bib22
  article-title: Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)81753-5
  contributor:
    fullname: Lu
– volume: 131
  start-page: 174
  year: 2007
  ident: 10.1016/j.cub.2017.06.016_bib5
  article-title: Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function
  publication-title: Cell
  doi: 10.1016/j.cell.2007.08.003
  contributor:
    fullname: Lécuyer
SSID ssj0012896
Score 2.3970742
Snippet How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by...
SourceID pubmedcentral
hal
crossref
pubmed
elsevier
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2101
SubjectTerms Animals
asymmetric cell division
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila melanogaster - physiology
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Larva - genetics
Larva - physiology
Life Sciences
Male
Mitosis
mRNA localization
nanobody
Neural Stem Cells - metabolism
neuroblasts
polarity
RNA, Messenger - genetics
RNA, Messenger - metabolism
Title Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA
URI https://dx.doi.org/10.1016/j.cub.2017.06.016
https://www.ncbi.nlm.nih.gov/pubmed/28690114
https://hal.science/hal-04736818
https://pubmed.ncbi.nlm.nih.gov/PMC5526833
Volume 27
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT-MwELZYENJeVjwW6PKQhTghRW1ixybHUkAttBxgQb1FtmPTIEhRtyDtv2fGSSoiIQ5cosRxLGtszXwZz3xDyJHicYa4PJBayYBrowLFhQoiw4Tg1jit0KE_uhb9O345jsdLpFfnwmBYZaX7S53utXXV0q6k2X7J8_atJ0sD-xbCJgUNC3p4BbnMYWuvdE_vr4aLwwT4p_BHltA_wA_qw00f5mVeNQZ4Sc_iiVXPPzdPPyYYJ7kwUs0Ayg8W6WKN_KqgJO2Ws10nS7bYIKtlccn_m2QyUsgFgYQalk4dHeUz9BrQIVqvKvuS5gU9m_lSBvmTop6pQwOenv-jgwIU15vFG0xS9ukPFL22FCAj7U0fCkCp9Pnmuvub3F2c_-31g6quQmB4HM0D5pxw_KSTWaM7TkrpDMAcnTDAC1nIQO0YZhkzzmGirMhOIi44HjcmwijpOmyLLBfTwu4QCvAmUTpLNI9CbnSmWAwDAMSIbGKUjVrkuBZn-lLSZ6R1XNljCrJPUfYpxtaFokV4LfC0sQdSUO9ffXYIi7MYHvmy-91him0dLpkASPIWtsh2uWSLfpGvxRXyFpGNxWwM1HxT5BNPwB0jRw5jf743313yE5_QQRzxPbI8n73afUA2c31Q7dwDwPaDK7gOxqfvWGD5XA
link.rule.ids 230,314,780,784,885,3506,27569,27924,27925,45663,45874
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6VVoheEO8uTwtxQop2Ezt2c1wWqi3N7gFa1JtlOzYbBNlq2Vbi3zPjJCsiIQ7cIr9kja2ZL56ZbwDeGJFXhMsTZY1KhHUmMUKaJHNcSuFdsIYe9BdLOb8QHy_zyz2Y9bkwFFbZ6f5Wp0dt3bWMO2mOr-p6_DmSpaF9S_GSooZFPXyAaEDiZT-YvvtyVu6cCfhPEV2WOD6hCb1zM4Z5uWtLAV4qsnhS1fO_m6dbK4qT3BmpYQDlHxbp5B7c7aAkm7a7vQ97vnkAt9vikr8ewmphiAuCCDU8Wwe2qDf0asBKsl5d9iWrG_Z-E0sZ1N8Ni0wdFvH09ic7bVBx3Xj6oCTlmP7A6NWWIWRks_XXBlEq-_FpOX0EFycfzmfzpKurkDiRZ9uEhyCDOJ5U3tlJUEoFhzDHFhzxQpVyVDuOe85dCJQoK6vjTEhB7sZCOqPChD-G_Wbd-CNgCG8KY6vCiiwVzlaG57gAQozMF874bARve3Hqq5Y-Q_dxZd80yl6T7DXF1qVyBKIXuB7cAY3q_V_TXuPh7JYnvuz5tNTUNhGKS4QkN-kInrRHthuXxVpcqRiBGhzmYKFhT1OvIgF3Thw5nD_9v_2-gjvz80Wpy9Pl2TM4pB56LM7Ec9jfbq79C0Q5W_uyu8W_AV7X-kQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Maintenance+of+Miranda+Localization+in+Drosophila+Neuroblasts+Involves+Interaction+with+the+Cognate+mRNA&rft.jtitle=Current+biology&rft.au=Ramat%2C+Anne&rft.au=Hannaford%2C+Matthew&rft.au=Januschke%2C+Jens&rft.date=2017-07-24&rft.pub=Cell+Press&rft.issn=0960-9822&rft.eissn=1879-0445&rft.volume=27&rft.issue=14&rft.spage=2101&rft.epage=2111.e5&rft_id=info:doi/10.1016%2Fj.cub.2017.06.016&rft_id=info%3Apmid%2F28690114&rft.externalDBID=PMC5526833
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0960-9822&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0960-9822&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0960-9822&client=summon