Transposable elements for efficient manipulation of a wide range of Gram-negative bacteria: promoter probes and vectors for foreign genes

• Published in: Gene Vol. 85; no. 1; pp. 83 - 89
• Main Authors: Joseph-Liauzun, Evelyne, Fellay, Rémy, Chandler, Michael
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 21.12.1989; Amsterdam Elsevier; New York, NY
• Subjects: Base Sequence; Biological and medical sciences; Biotechnology; Cloning, Molecular; DNA Probes; DNA Transposable Elements; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Genes; Genetic engineering; Genetic technics; Genetic Vectors; Gram-Negative Bacteria - genetics; inducible promoters; insertion sequence; IS1; Methods. Procedures. Technologies; Molecular Sequence Data; Mutation; omegon-Km; Paracoccus denitrificans - genetics; plant pathogen; Plasmids; Promoter Regions, Genetic; Pseudomonas - genetics; Recombinant DNA; Restriction Mapping; Rhizobium - genetics; transposition; Xanthomonas - genetics; Xanthomonas campestris; Zymomonas mobilis; Km; omegon-Km; oriV; IS1; nt; Ks; Cm; Recombinant DNA; bp; Tc; transposition; R; IRL and IRR; cat; kb; X; oligo; oriT; Z; Zymomonas mobilis; RBS; plant pathogen; inducible promoters; IS; Rif; Xanthomonas campestris; TEA; insertion sequence; XGal; PolIk; Sm; Chromosomal aberration; Pseudomonadales; Heterologous system; Chromosome insertion; Transcription promoter; Transposon; Site directed mutagenesis; Transposable element; Method; DNA; Abnormal chromosome; Pseudomonadaceae; Bacteria; Genetic engineering; Molecular cloning; Vector; Hybrid gene; Gram negative bacteria

We describe here the construction and use of a series of modified transposons based on the insertion sequence IS1. Like their parent, omegon-Km [Fellay et al., Gene 76 (1989) 215–226], these elements permit efficient insertional mutagenesis of a variety of Gram-negative bacteria. The presence of a f...