Detection of decreased granules in neutrophils by automated hematology analyzers XR-1000 and UniCel DxH 800

• Published in: Laboratory Medicine Vol. 55; no. 6; pp. 768 - 775
• Main Authors: Kato, Yosuke, Sakamoto, Daisuke, Ohnishi, Hiroaki, Taki, Tomohiko
• Format: Journal Article
• Language: English
• Published: US Oxford University Press (OUP) 04.11.2024; Oxford University Press
• Subjects: Adult; Aged; Aged, 80 and over; Automation; Automation, Laboratory - methods; Cytoplasmic Granules; Female; Hematology; Humans; Male; Middle Aged; Myelodysplastic Syndromes - diagnosis; Neutrophils; XR-1000; flow cytometry; hypogranulation; cell population data; myelodysplastic syndromes; UniCel DxH 800
• ISSN: 0007-5027; 1943-7730; 1943-7730
• DOI: 10.1093/labmed/lmae047

Abstract Objective This study aimed to investigate the utility of neutrophil-related cell population data obtained by automated hematology analyzers in assessing myelodysplastic syndrome cases with decreased granules in neutrophils. Methods A total of 108 subjects were classified into normal granule (n = 35), hypogranulation (n = 37), or hypergranulation (n = 36) groups. Neutrophil cell area and granule area were measured by ImageJ. All samples were analyzed on the XR-1000 and UniCel DxH 800, and neutrophil-related parameters were compared among the 3 groups. Results Neutrophil cell area and the ratio of the granular area showed significant differences among the 3 groups; they were the highest in the hypergranulation group and lowest in the hypogranulation group. XR-1000 data showed significant differences in NE-SFL and NE-FSC among the 3 groups (P < .0001). NE-SFL and NE-FSC discriminated most accurately hypogranulation group against other groups. UniCel DxH 800 data showed significant differences in MN-V-NE, MN-MALS-N, MN-UMALS-NE, SD-UMALS-NE (P <.01), MN-LMALS-NE, and SD-LMALS-NE (P <.05) among the 3 groups. The combination of SD-V-NE and SD-LMALS-NE discriminated most accurately the hypogranulation group against the other groups. Conclusion NE-SFL and NE-FSC and the combination of SD-V-NE and SD-LMALS-NE are useful in detecting cases with decreased granules in neutrophils.