Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria

• Published in: Plant physiology and biochemistry Vol. 38; no. 10; pp. 773 - 779
• Main Authors: De Leonardis, Silvana, Dipierro, Nunzio, Dipierro, Silvio
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 2000; Elsevier
• Subjects: Agronomy. Soil science and plant productions; ascorbate; ascorbate peroxidase; Biological and medical sciences; CHEMICOPHYSICAL PROPERTIES; Economic plant physiology; Enzymes; Fundamental and applied biological sciences. Psychology; HORTALIZAS (PLANTAS); Metabolism; MITOCHONDRIA; MITOCHONDRIE; MITOCONDRIA; Nutrition. Photosynthesis. Respiration. Metabolism; PEROXIDASAS; PEROXIDASES; PEROXYDASE; Plant physiology and development; PLANTE LEGUMIERE; PROPIEDADES FISICOQUIMICAS; PROPRIETE PHYSICOCHIMIQUE; reactive oxygen species; SOLANUM TUBEROSUM; VEGETABLE CROPS; NEM, N-ethylmaleimide; ascorbate peroxidase; p-HMB, p-hydroxymercuribenzoate; Solanum tuberosum; mitochondria; AsA, ascorbic acid or ascorbate; AFR, ascorbate-free radical; SOD, superoxide dismutase; mAPX, mitochondrial ascorbate peroxidase; reactive oxygen species; ascorbate; ROS, reactive oxygen species; DHA, dehydroascorbate; cAPX, cytosolic ascorbate peroxidase; APX, ascorbate peroxidase; Purification; Enzyme; L-Ascorbate peroxidase; Characterization; Mitochondria; Enzymatic activity; Peroxidases; Dicotyledones; Angiospermae; Tuber; Spermatophyta; Oxidoreductases; Tuber plant; Solanaceae; Catalyst activity

Ascorbate peroxidase (APX) has been purified from potato tuber ( Solanum tuberosum L.) mitochondria. The potato enzyme appeared to be localized inside mitochondria. The mitochondrial APX was purified to homogeneity and its physico-chemical and kinetic properties were compared with those of the cytos...