Development and Preclinical Characterization of a Humanized Antibody Targeting CXCL12

• Published in: Clinical cancer research Vol. 19; no. 16; pp. 4433 - 4445
• Main Authors: CUILING ZHONG, JIANYONG WANG, QUINTANA, Leah, GRIBLING, Peter, SUTO, Eric, BARCK, Kai, CORPUZ, Racquel, YAO, Jenny, TAKKAR, Rashi, LEE, Wyne P, DAMICO-BEYER, Lisa A, CARANO, Richard D, BING LI, ADAMS, Camellia, KELLEY, Robert F, WEIRU WANG, FERRARA, Napoleone, HONG XIANG, ULTSCH, Mark, COONS, Mary, WONG, Terence, CHIANG, Nancy Y, CLARK, Suzy, CLARK, Robyn
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.08.2013
• Subjects: Angiogenesis Inhibitors - administration & dosage; Angiogenesis Inhibitors - chemistry; Angiogenesis Inhibitors - pharmacology; Animals; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Antibodies, Monoclonal - pharmacology; Antibodies, Monoclonal, Humanized - administration & dosage; Antibodies, Monoclonal, Humanized - chemistry; Antibodies, Monoclonal, Humanized - pharmacology; Antineoplastic agents; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Arthritis, Experimental - drug therapy; Arthritis, Experimental - metabolism; Biological and medical sciences; Cell Line, Tumor; Chemokine CXCL12 - antagonists & inhibitors; Chemokine CXCL12 - chemistry; Chemokine CXCL12 - metabolism; Cricetinae; Disease Models, Animal; Drug Evaluation, Preclinical; Drug Synergism; Epitope Mapping; Female; General aspects; Humans; Medical sciences; Mice; Models, Molecular; Neoplasm Metastasis; Neoplasms - drug therapy; Neoplasms - pathology; Pharmacology. Drug treatments; Protein Conformation; Tumor Burden - drug effects; Vascular Endothelial Growth Factor A - antagonists & inhibitors; Xenograft Model Antitumor Assays; Chemokine CXCL12; Rodentia; Preclinical study; Monoclonal antibody; Malignant tumor; In vitro; Biological activity; Inflammatory disease; In vivo; Vertebrata; Mammalia; Animal; Humanized antibody; Hamster; Cancer

Our goal was to develop a potent humanized antibody against mouse/human CXCL12. This report summarized its in vitro and in vivo activities. Cell surface binding and cell migration assays were used to select neutralizing hamster antibodies, followed by testing in several animal models. Monoclonal ant...