Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine

• Published in: Clinica chimica acta Vol. 202; no. 1; pp. 11 - 22
• Main Authors: Silver, Anne C., Lamb, Edmund, Cattell, William R., Dawnay, Anne B.St.J.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 14.10.1991; Elsevier
• Subjects: Adult; Albuminuria; Analytical, structural and metabolic biochemistry; Binding and carrier proteins; Biological and medical sciences; Biomarkers - blood; Biomarkers - urine; Chromatography, Affinity - methods; Diabetes Mellitus - blood; Diabetes Mellitus - urine; Fructosamine; Fundamental and applied biological sciences. Psychology; Glycated albumin; Glycated protein; Glycosuria; Glycosylation; Hexosamines - blood; Humans; m-Aminophenyl boronate affinity chromatography; Middle Aged; Proteins; Radioimmunoassay - methods; Reference Values; Serum Albumin - analysis; Urine protein; Glycated albumin; Glycated protein; m-Aminophenyl boronate affinity chromatography; Urine protein; Human; Urine; Biological fluid; Affinity chromatography; Diabetes mellitus; Radioimmunoassay; Clinical biology; Albumin; Biological marker; Serum; Quantitative analysis

Affinity chromatography on m-aminophenyl boronate columns together with albumin measurement by radioimmunoassay has been validated as a method for determining glycated albumin in serum and urine. Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of re...