A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica

• Published in: Journal of microbiological methods Vol. 70; no. 3; pp. 493 - 502
• Main Authors: Bordes, Florence, Fudalej, Franck, Dossat, Valérie, Nicaud, Jean-Marc, Marty, Alain
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.09.2007; Elsevier Science; Elsevier
• Subjects: Biochemistry, Molecular Biology; Biological and medical sciences; Biotechnology; Directed evolution; Enzyme engineering; Expression system; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation, Fungal; High-throughput screening; Life Sciences; Lipase - genetics; Lipase - metabolism; Methods. Procedures. Technologies; Molecular biology; Mutagenesis, Insertional; Plasmids - genetics; Production of selected enzymes; Protein engineering; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Transformation, Genetic; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; High-throughput screening; Expression system; Protein engineering; Directed evolution; Yarrowia lipolytica; Ascomycota; High throughput screening; Yeast; Eukaryote; Host; Fungi; Improvement; Evolution; Recombinant protein

Development of a high-throughput eukaryotic screening procedure is important to increase success in obtaining improved enzymes through directed enzyme evolution. This procedure was developed for the yeast Yarrowia lipolytica which becomes the second eukaryotic host for this purpose. The extracellula...