Trait stacking via targeted genome editing

• Published in: Plant biotechnology journal Vol. 11; no. 9; pp. 1126 - 1134
• Main Authors: Ainley, William M., Sastry‐Dent, Lakshmi, Welter, Mary E., Murray, Michael G., Zeitler, Bryan, Amora, Rainier, Corbin, David R., Miles, Rebecca R., Arnold, Nicole L., Strange, Tonya L., Simpson, Matthew A., Cao, Zehui, Carroll, Carley, Pawelczak, Katherine S., Blue, Ryan, West, Kim, Rowland, Lynn M., Perkins, Douglas, Samuel, Pon, Dewes, Cristie M., Shen, Liu, Sriram, Shreedharan, Evans, Steven L., Rebar, Edward J., Zhang, Lei, Gregory, Phillip D., Urnov, Fyodor D., Webb, Steven R., Petolino, Joseph F.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.12.2013
• Subjects: Corn; Crops; Crops, Agricultural; Deoxyribonucleic acid; designed zinc finger nucleases; DNA; Editing; Embryos; Endonucleases - genetics; Endonucleases - metabolism; Gene sequencing; gene targeting; Gene Targeting - methods; Genes; Genetic Linkage; Genetic transformation; Genome editing; Genome, Plant - genetics; Genomes; Genomics; Herbicide Resistance; Herbicides; Herbicides - pharmacology; Integration; Microparticles; Modular engineering; Nuclease; Nucleotide sequence; Phenotype; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; Rice; transgene stacking; Transgenes; Transgenic plants; Zea mays; Zea mays - genetics; Zinc finger proteins; Zinc Fingers; United States > US; transgene stacking; gene targeting; designed zinc finger nucleases

Summary Modern agriculture demands crops carrying multiple traits. The current paradigm of randomly integrating and sorting independently segregating transgenes creates severe downstream breeding challenges. A versatile, generally applicable solution is hereby provided: the combination of high‐effic...