Human serum leads to differentiation of human hepatoma cells, restoration of very‐low‐density lipoprotein secretion, and a 1000‐fold increase in HCV Japanese fulminant hepatitis type 1 titers

• Published in: Hepatology (Baltimore, Md.) Vol. 58; no. 6; pp. 1907 - 1917
• Main Authors: Steenbergen, Rineke H.G., Joyce, Michael A., Thomas, Bradley S., Jones, Daniel, Law, John, Russell, Rodney, Houghton, Michael, Tyrrell, D. Lorne
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.12.2013
• Subjects: Animals; Apolipoproteins B - secretion; Cadherins - biosynthesis; Carcinoma, Hepatocellular - pathology; Cattle; Cell Differentiation; Cell Line, Tumor; Cholesterol; Claudin-1 - biosynthesis; Culture Media; Fatty acids; Hepacivirus - growth & development; Hepatitis C virus; Hepatocytes - cytology; Hepatocytes - drug effects; Hepatocytes - virology; Hepatology; Humans; Lipoproteins - secretion; Liver Neoplasms; Occludin - biosynthesis; Serum

In this study, we differentiated the human hepatoma cell line Huh7.5 by supplementing tissue culture media with human serum (HS) and examined the production of hepatitis C virus (HCV) by these cells. We compared the standard tissue culture protocol, using media supplemented with 10% fetal bovine ser...