Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism

• Published in: Food science and biotechnology Vol. 28; no. 2; pp. 547 - 553
• Main Authors: Kim, Leeseon, Kim, Ji Yeon
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.04.2019; Springer Nature B.V; 한국식품과학회
• Subjects: anti-inflammatory activity; Chemistry; Chemistry and Materials Science; Chondrocytes; collagen; Collagen (type II); Curcumin; Cyclooxygenase-2; Cytokines; enzyme inhibitors; Food Science; Gene expression; Hyaluronic acid; IL-1β; inducible nitric oxide synthase; Inflammation; Interleukin 6; Interleukin 8; interleukin-1beta; Lecithin; lecithins; Macrophages; Metalloproteinase; metalloproteinases; Nitric oxide; Nitric-oxide synthase; Nutrition; Polypeptides; Prostaglandin E2; prostaglandin synthase; prostaglandins; Proteoglycans; secretion; solubility; Tissue inhibitor of metalloproteinase 1; tumor necrosis factor-alpha; Tumor necrosis factor-TNF; Tumor necrosis factor-α; 식품과학; Chondroprotective effect; Curcumin; Lecithin complex; Ant-inflammatory effect; Osteoarthritis
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-018-0470-6

A complex of curcumin and lecithin developed to improve the solubility of curcumin, enhanced its chondroprotective effect via an anti-inflammatory mechanism. In macrophage, proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, prostaglandin E2 (PGE2), and nitric oxide (NO) were quantified. In addition, the activity of nuclear factor (NF)-κB was examined. With chondrocytes, inflammatory mediators were assessed by measuring the secretion levels of IL-6, IL-8, and PGE2, also the mRNA expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Metalloproteinases (MMPs), tissue inhibitor of metalloprotease (TIMP)-1, type II collagen (COL2), proteoglycan (PG), and hyaluronic acid (HA) were measured with respect to the articulation surface. The complex promoted the anti-inflammatory effect by the inhibition of inflammatory mediators. In addition, mRNA expression levels ameliorated. Furthermore, it was effective in decreasing extracellular secretion of polypeptides, also corresponding intracellular MMPs and TIMP-1. In conclusion, the complex may be developed as a functional supplement to maintain articulation health.