Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis)

• Published in: Journal of fish biology Vol. 98; no. 2; pp. 399 - 414
• Main Authors: Brys, Rein, Halfmaerten, David, Neyrinck, Sabrina, Mauvisseau, Quentin, Auwerx, Johan, Sweet, Michael, Mergeay, Joachim
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2021; Wiley Subscription Services, Inc
• Subjects: Animals; Aquaria; Aquariums; Belgium; conservation; cryptic species; Cypriniformes - genetics; ddPCR versus qPCR analyses; Deoxyribonucleic acid; Detection; detection limit; Dilution; DNA; droplets; Ecosystem; eDNA detection; endangered; Endangered Species; Environmental DNA; Environmental DNA - analysis; Environmental DNA - genetics; Environmental Monitoring; extinction; Fish; Fish conservation; Fresh Water - chemistry; Freshwater fishes; habitats; Harbors; Harbours; Misgurnus fossilis; Monitoring; Monitoring methods; Nucleotide sequence; PCR; Population Density; Quality assessment; Quality control; Rare species; Real-Time Polymerase Chain Reaction; risk; Sampling; Sensitivity; Species extinction; Surveying; surveys; water sampling; Weather; Wildlife conservation; Belgium; endangered; conservation; eDNA detection; water sampling; ddPCR versus qPCR analyses; cryptic species

The European weather loach (Misgurnus fossilis) is a cryptic and poorly known fish species of high conservation concern. The species is experiencing dramatic population collapses across its native range to the point of regional extinction. Although environmental DNA (eDNA)‐based approaches offer cle...