Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis) - Brys, Rein; Halfmaerten, David; Neyrinck, Sabrina; Mauvisseau, Quentin; Auwerx, Johan; Sweet, Michael; Mergeay, Joachim (Journal of fish biology, 2021)
Keywords: Animals; Aquaria; Aquariums; Belgium; conservation; cryptic species; Cypriniformes - genetics; ddPCR versus qPCR analyses; Deoxyribonucleic acid; Detection; detection limit; Dilution; DNA; droplets; Ecosystem; eDNA detection; endangered; Endangered Species; Environmental DNA; Environmental DNA - analysis; Environmental DNA - genetics; Environmental Monitoring; extinction; Fish; Fish conservation; Fresh Water - chemistry; Freshwater fishes; habitats; Harbors; Harbours; Misgurnus fossilis; Monitoring; Monitoring methods; Nucleotide sequence; PCR; Population Density; Quality assessment; Quality control; Rare species; Real-Time Polymerase Chain Reaction; risk; Sampling; Sensitivity; Species extinction; Surveying; surveys; water sampling; Weather; Wildlife conservation; Belgium; endangered; conservation; eDNA detection; water sampling; ddPCR versus qPCR analyses; cryptic species