Osmolyte controlled fibrillation kinetics of insulin: New insight into fibrillation using the preferential exclusion principle

• Published in: Biotechnology progress Vol. 25; no. 5; pp. 1508 - 1514
• Main Authors: Nayak, Arpan, Lee, Chuang-Chung, McRae, Gregory J., Belfort, Georges
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2009; Wiley
• Subjects: amyloid; Amyloid - chemistry; Biological and medical sciences; Biotechnology; Carbohydrates - chemistry; Computer Simulation; Fundamental and applied biological sciences. Psychology; Humans; Insulin - chemistry; Insulin - metabolism; Kinetics; Models, Chemical; osmolyte; protein aggregation; Protein Multimerization; Temperature; Osmolyte; Insulin; Kinetics

Amyloid proteins are converted from their native‐fold to long β‐sheet‐rich fibrils in a typical sigmoidal time‐dependent protein aggregation curve. This reaction process from monomer or dimer to oligomer to nuclei and then to fibrils is the subject of intense study. The main results of this work are...