Serum analysis method combining cellulose acetate membrane purification with surface-enhanced Raman spectroscopy for non-invasive HBV screening

• Published in: IET nanobiotechnology Vol. 14; no. 1; pp. 98 - 104
• Main Authors: Xu, Yunchao, Wang, Yunyi, Lin, Huijin, Liu, Xiaokun, Zheng, Zuci, Wang, Tingyin, Feng, Shangyuan
• Format: Journal Article
• Language: English
• Published: United States The Institution of Engineering and Technology 01.02.2020
• Subjects: Biomarkers - blood; biomembranes; Blood Proteins - analysis; Cellulose - analogs & derivatives; Cellulose - chemistry; cellulose acetate membrane purification; cellulose acetate membrane‐purified human serum; diagnostic sensitivity; diseases; HBV patient; Hepatitis B - blood; Hepatitis B - diagnosis; Hepatitis B virus; Humans; linear discriminate analysis; membrane protein purification; Metal Nanoparticles - chemistry; nanomedicine; nanoparticles; noninvasive HBV screening; patient diagnosis; Principal Component Analysis; principal components analysis; proteins; purification; rapid HBV screening method; reproducible serum protein SERS spectra; Research Article; serum analysis method; silver; Silver - chemistry; silver nanoparticle‐based surface‐enhanced Raman scattering spectroscopy; Spectrum Analysis, Raman - methods; surface enhanced Raman scattering; surface‐enhanced Raman spectroscopy; Viral Proteins - blood; noninvasive HBV screening; nanomedicine; diseases; reproducible serum protein SERS spectra; principal components analysis; biomembranes; rapid HBV screening method; nanoparticles; serum analysis method; surface enhanced Raman scattering; proteins; purification; silver nanoparticle-based surface-enhanced Raman scattering spectroscopy; cellulose acetate membrane-purified human serum; membrane protein purification; diagnostic sensitivity; cellulose acetate membrane purification; HBV patient; silver; Hepatitis B virus; patient diagnosis; principal component analysis; surface-enhanced Raman spectroscopy; linear discriminate analysis
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2019.0274

A highly sensitive, non-invasive, and rapid HBV (Hepatitis B virus) screening method combining membrane protein purification with silver nanoparticle-based surface-enhanced Raman scattering (SERS) spectroscopy was developed in this study. Reproducible serum protein SERS spectra were obtained from cellulose acetate membrane-purified human serum from 94 HBV patients and 89 normal groups. Tentative assignments of serum protein SERS spectra showed that the HBV patients primarily led to specific biomedical changes of serum protein. Principal components analysis and linear discriminate analysis were introduced to analyse the obtained spectra, with the diagnostic sensitivity of 92.6% and specificity of 77.5% were achieved for differentiating HBV patients from normal groups.