Identification of Pseudomonas aeruginosa genes required for epithelial cell injury

• Published in: Molecular microbiology Vol. 24; no. 6; pp. 1249 - 1262
• Main Authors: Jung Kang, Pil, Hauser, Alan R., Apodaca, Gerard, Fleiszig, Suzanne M. J., Wiener‐Kronish, Jeanine, Mostov, Keith, Engel, Joanne N.
• Format: Journal Article
• Language: English
• Published: Oxford UK Blackwell Science Ltd 01.06.1997
• Subjects: Amino Acid Sequence; Animals; Bacterial Adhesion; Bacterial Proteins - genetics; Cell Line; DNA Transposable Elements; Dogs; Epithelial Cells; Fimbriae, Bacterial; Genes, Bacterial; Lung - cytology; Lung - microbiology; Molecular Sequence Data; Mutagenesis; Polymerase Chain Reaction; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - pathogenicity; Sequence Analysis, DNA; Virulence - genetics

We have developed a simple, reproducible and rapid genetic screen for Pseudomonas aeruginosa‐induced epithelial cell cytotoxicity in cultures of MDCK cells. This screen was used to isolate isogenic transposon‐tagged non‐cytotoxic mutants of a cytotoxic and lung‐virulent strain of P. aeruginosa (PA10...