De novo main-chain modeling with MAINMAST in 2015/2016 EM Model Challenge

• Published in: Journal of structural biology Vol. 204; no. 2; pp. 351 - 359
• Main Authors: Terashi, Genki, Kihara, Daisuke
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2018
• Subjects: confidence score; Cryo-EM; Cryoelectron Microscopy - methods; CryoEM Model Challenge; Electron microscopy; Main-chain trace; MAINMAST; Map interpretation; Mean shifting algorithm; Minimum spanning tree; Protein Conformation; Protein structure modeling; Proteins - chemistry; Rosetta; Software; Protein structure modeling; Main-chain trace; Mean shifting algorithm; confidence score; Cryo-EM; Map interpretation; Minimum spanning tree; Electron microscopy; CryoEM Model Challenge; MAINMAST; Rosetta

Protein tertiary structure modeling is a critical step for the interpretation of three dimensional (3D) election microscopy density. Our group participated the 2015/2016 EM Model Challenge using the MAINMAST software for a de novo main chain modeling. The software generates local dense points using...