Portable wide-field femtoliter-chamber imaging system for point-of-care digital bioanalysis
Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of c...
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| Published in | iScience Vol. 27; no. 9; p. 110868 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
20.09.2024
Elsevier |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2589-0042 2589-0042 |
| DOI | 10.1016/j.isci.2024.110868 |
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| Abstract | Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases.
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•Developed a portable wide-field fluorescence imaging system for digital bioanalysis•Developed low-cost and durable consumables for digital bioanalysis•Developed a stable storage method for the assay reagents used in digital bioanalysis•Performed a point-of-care digital bioanalysis in a hospital setting
Applied sciences; Bioelectronics; Biotechnology |
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| AbstractList | Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases.
[Display omitted]
•Developed a portable wide-field fluorescence imaging system for digital bioanalysis•Developed low-cost and durable consumables for digital bioanalysis•Developed a stable storage method for the assay reagents used in digital bioanalysis•Performed a point-of-care digital bioanalysis in a hospital setting
Applied sciences; Bioelectronics; Biotechnology Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases.Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases. Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases. Recently, digital bioanalysis using femtoliter (fL)-chamber arrays has significantly improved the sensitivity, accuracy, and throughput of conventional nucleic acid and antigen tests, with great potential for the diagnosis of infectious diseases and underlying disorders. However, the large size of conventional platforms with costly assay consumables for digital bioanalysis complicates its use in point-of-care testing (POCT). To solve these problems, in this study, we developed a wide-field fL-chamber imaging system (COWFISH2), a portable wide-field femtoliter-chamber imaging system (footprint: 14 × 22 cm), by redesigning various electronic controls and optical systems of COWFISH, accompanied by the development of low-cost and durable consumables for digital bioanalysis. As a proof of concept, the point-of-care digital bioanalysis was successfully performed in a hospital setting, using amplification-free multiplex digital RNA detection of SARS-CoV-2, influenza A virus, and influenza B virus. Collectively, COWFISH2 will facilitate versatile and convenient digital bioanalysis in POCT, contributing to the improvement of public health, including the prevention of infectious diseases. • Developed a portable wide-field fluorescence imaging system for digital bioanalysis • Developed low-cost and durable consumables for digital bioanalysis • Developed a stable storage method for the assay reagents used in digital bioanalysis • Performed a point-of-care digital bioanalysis in a hospital setting Applied sciences; Bioelectronics; Biotechnology |
| ArticleNumber | 110868 |
| Author | Kogo, Yasushi Ando, Jun Noda, Takeshi Iida, Tatsuya Yoshimura, Mami Toyoda, Masashi Watanabe, Rikiya Makino, Asami Nakano, Masahiro Shinoda, Hajime |
| Author_xml | – sequence: 1 givenname: Tatsuya surname: Iida fullname: Iida, Tatsuya organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 2 givenname: Jun surname: Ando fullname: Ando, Jun organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 3 givenname: Mami surname: Yoshimura fullname: Yoshimura, Mami organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 4 givenname: Asami surname: Makino fullname: Makino, Asami organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 5 givenname: Masahiro surname: Nakano fullname: Nakano, Masahiro organization: Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan – sequence: 6 givenname: Yasushi surname: Kogo fullname: Kogo, Yasushi organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 7 givenname: Hajime surname: Shinoda fullname: Shinoda, Hajime organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan – sequence: 8 givenname: Masashi surname: Toyoda fullname: Toyoda, Masashi organization: Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, Japan – sequence: 9 givenname: Takeshi surname: Noda fullname: Noda, Takeshi organization: Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan – sequence: 10 givenname: Rikiya orcidid: 0000-0001-7146-2745 surname: Watanabe fullname: Watanabe, Rikiya email: rikiya.watanabe@riken.jp organization: Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan |
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