Diverse patterns of poly(A) tail elongation and shortening of murine maternal mRNAs from fully grown oocyte to 2-cell embryo stages

• Published in: Biochemical and biophysical research communications Vol. 336; no. 4; pp. 1181 - 1189
• Main Authors: Sakurai, Takayuki, Sato, Masahiro, Kimura, Minoru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.11.2005
• Subjects: Animals; cDNA library; Cytoplasmic polyadenylation; Deadenylation; Embryo, Mammalian - physiology; Female; Fertilized egg; Gene Expression Profiling; Gene Expression Regulation, Developmental; Maternal RNA; Mice; Mouse; Oocyte; Oocytes - physiology; Poly A - genetics; Poly(A) tail; Polyadenylation; Preimplantation embryo; RNA, Messenger, Stored - biosynthesis; RNA, Messenger, Stored - genetics; Translational control; Deadenylation; Mouse; Fertilized egg; Maternal RNA; Poly(A) tail; Preimplantation embryo; Cytoplasmic polyadenylation; Translational control; Oocyte; cDNA library

We previously succeeded in constructing a cDNA library, CPF7, enriched with cDNA derived from maternal RNAs with the extended poly(A) tail in mouse fertilized eggs. In this study, we performed RNA blot analysis to examine the elongation in maternal RNAs using 20 representative clones isolated from C...