Construction of an infectious plasmid clone of Muscovy duck parvovirus by TA cloning and creation of a partially attenuated strain
Muscovy duck parvovirus (MDPV) infection is a highly contagious and fatal disease of Muscovy ducklings. The infectious clone methodology is a valuable tool to study the pathogenic mechanisms of viruses, but no infectious clone of MDPV is yet available. In this study, a plasmid clone containing the f...
Saved in:
Published in | Avian pathology Vol. 44; no. 2; pp. 124 - 128 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Taylor & Francis
04.03.2015
Taylor & Francis Ltd |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Muscovy duck parvovirus (MDPV) infection is a highly contagious and fatal disease of Muscovy ducklings. The infectious clone methodology is a valuable tool to study the pathogenic mechanisms of viruses, but no infectious clone of MDPV is yet available. In this study, a plasmid clone containing the full-length genome of MDPV was constructed using the TA cloning methodology. This MDPV clone was found to be infectious after transfection of primary Muscovy duck embryo fibroblast cells and passage in embryonated Muscovy duck eggs. Site-directed mutagenesis showed that the K75N mutation in the VP1 protein of MDPV resulted in the partial attenuation of the virus. The availability of an MDPV infectious clone can facilitate investigation of the pathogenic mechanisms of MDPV and development of vaccines against diseases caused by MDPV. |
---|---|
AbstractList | Muscovy duck parvovirus (MDPV) infection is a highly contagious and fatal disease of Muscovy ducklings. The infectious clone methodology is a valuable tool to study the pathogenic mechanisms of viruses, but no infectious clone of MDPV is yet available. In this study, a plasmid clone containing the full-length genome of MDPV was constructed using the TA cloning methodology. This MDPV clone was found to be infectious after transfection of primary Muscovy duck embryo fibroblast cells and passage in embryonated Muscovy duck eggs. Site-directed mutagenesis showed that the K75N mutation in the VP1 protein of MDPV resulted in the partial attenuation of the virus. The availability of an MDPV infectious clone can facilitate investigation of the pathogenic mechanisms of MDPV and development of vaccines against diseases caused by MDPV. |
Author | Lu, H.-M Yen, T.-Y Chang, P.-C Shien, J.-H Li, K.-P Ou, S.-C |
Author_xml | – sequence: 1 fullname: Yen, T.-Y – sequence: 2 fullname: Li, K.-P – sequence: 3 fullname: Ou, S.-C – sequence: 4 fullname: Shien, J.-H – sequence: 5 fullname: Lu, H.-M – sequence: 6 fullname: Chang, P.-C |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25609267$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkctu1DAYhS1URC_wCIAlNt1Ma8f3HdWIAlKrLmjXluPYlUtiD3YyVbY8OU5nWiE2rHz7zjn-dY7BQUzRAfAeozOMJDpHBAlFmThrEGb1Ckmi1CtwhClnK0KIPPhrfwiOS3lACHHGmjfgsGEcqYaLI_B7nWIZ82THkCJMHpoIQ_RuOU8FbnpThtBB29f05fl6KjZtZ9hN9ifcmLxN25Ar2M7w9uIJC_G-mlRJdubFdEHHYPp-hmYcXZzM6DpYg02Ib8Frb_ri3u3XE3B3-eV2_W11dfP1-_riamUpw-Oqc4K6lkvMOFXUG9lw1BKhUB1GecklssS2jZeEd9gr0gmKSSsMtYw64QU5Aac7301OvyZXRj2EYl3fm-jqrBoLJQVFlKqKfvoHfUhTjvV3GnOOBaZUykqxHWVzKiU7rzc5DCbPGiO9lKSfS9JLSXpfUtV92LtP7eC6F9VzKxX4vANqEykP5jHlvtOjmfuUfTbRhqLJ_zI-7iy8Sdrc56q4-7EQCGGipGTkD_3krEw |
CitedBy_id | crossref_primary_10_1142_S1682648515500213 crossref_primary_10_1016_j_vetmic_2019_06_015 crossref_primary_10_1142_S1682648515500079 crossref_primary_10_14348_molcells_2016_0033 crossref_primary_10_1186_s12870_020_02710_y crossref_primary_10_3390_ani11113211 crossref_primary_10_1007_s00705_016_2879_7 crossref_primary_10_1186_s12985_019_1237_2 crossref_primary_10_2478_jvetres_2021_0063 |
Cites_doi | 10.1093/nar/19.5.1156 10.1080/03079459408419004 10.1099/0022-1317-82-4-919 10.1556/AVet.53.2005.1.8 10.1006/viro.1996.0367 10.1080/03079450094270 10.1016/j.jviromet.2014.02.014 10.1016/S1534-5807(01)00031-4 10.1006/viro.1995.1345 10.1007/BF01309459 10.1016/j.virol.2003.09.011 10.1128/JVI.79.17.11035-11044.2005 10.1080/03079450410001724067 10.1080/03079450802356979 10.1637/7172 10.1006/viro.1995.1514 |
ContentType | Journal Article |
Copyright | 2015 Houghton Trust Ltd 2015 Copyright Taylor & Francis Ltd. 2015 |
Copyright_xml | – notice: 2015 Houghton Trust Ltd 2015 – notice: Copyright Taylor & Francis Ltd. 2015 |
DBID | FBQ CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QL 7T5 7TM 7U7 7U9 C1K F1W H94 H95 K9. L.G M7N |
DOI | 10.1080/03079457.2015.1008399 |
DatabaseName | AGRIS Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Bacteriology Abstracts (Microbiology B) Immunology Abstracts Nucleic Acids Abstracts Toxicology Abstracts Virology and AIDS Abstracts Environmental Sciences and Pollution Management ASFA: Aquatic Sciences and Fisheries Abstracts AIDS and Cancer Research Abstracts Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources ProQuest Health & Medical Complete (Alumni) Aquatic Science & Fisheries Abstracts (ASFA) Professional Algology Mycology and Protozoology Abstracts (Microbiology C) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Aquatic Science & Fisheries Abstracts (ASFA) Professional Virology and AIDS Abstracts Toxicology Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Nucleic Acids Abstracts ASFA: Aquatic Sciences and Fisheries Abstracts AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Immunology Abstracts Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Environmental Sciences and Pollution Management |
DatabaseTitleList | MEDLINE Aquatic Science & Fisheries Abstracts (ASFA) Professional Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Veterinary Medicine |
EISSN | 1465-3338 |
EndPage | 128 |
ExternalDocumentID | 3618954731 10_1080_03079457_2015_1008399 25609267 1008399 US201500139885 |
Genre | ORIGINAL ARTICLES Research Support, Non-U.S. Gov't Journal Article |
GrantInformation_xml | – fundername: ATU plan from the Ministry of Education |
GroupedDBID | --- .7F .QJ 07X 0BK 0R~ 23N 2DF 30N 36B 3V. 3YN 4.4 53G 5GY 5VS 6J9 7X2 7X7 88A 88E 8FE 8FH 8FI 8FJ 8G5 AAAVI AAENE AAJMT AALDU AAMIU AAOAP AAPUL AAQRR ABCCY ABDBF ABFIM ABJNI ABJVF ABLIJ ABPEM ABPTK ABQHQ ABTAI ABUWG ABXUL ABXYU ACBBU ACDHJ ACGEJ ACGFO ACGFS ACPRK ACTIO ACZPZ ADBBV ADCVX ADGTB ADGTR ADOPC ADXPE AEGXH AEGYZ AEISY AENEX AEOZL AEPSL AEYOC AFDYB AFKRA AFKVX AFOLD AFRAH AFWLO AGDLA AGMYJ AHDLD AI. AIAGR AIJEM AIRXU AJBAX AJWEG AKBVH AKOOK ALMA_UNASSIGNED_HOLDINGS ALQZU APEBS APNXG AQRUH ATCPS AURDB AVBZW AWYRJ AZQEC BBNVY BENPR BFWEY BHPHI BLEHA BPHCQ BVXVI C0. C1A C5I CAG CCCUG CCPQU CE4 COF CS3 CWRZV DGEBU DKSSO DLOXE DU5 DWQXO EAP EAS EBD EBS EDH EJD EMB EMK EMOBN EPL ESX EYRJQ E~A E~B F5P FBQ FIJ FUNRP FVPDL FYUFA GNUQQ GTTXZ GUQSH HCIFZ HF~ HGUVV HMCUK HZ~ H~P IPNFZ J.P JEPSP KYCEM L84 LJTGL LK8 M0K M0L M1P M2O M4Z M7P NA5 NUSFT NX0 O9- OK1 OVD OWHGL P2P PCLFJ PQQKQ PROAC PSQYO RIG RNANH ROSJB RTWRZ S-T SNACF SV3 TEI TEORI TFL TFT TFW TGX TQWBC TTHFI TWF UB7 UKHRP UT5 UU3 V1K VH1 ZGOLN ~KM ~S~ ABBKH H13 ABPAQ AHDZW CGR CUY CVF ECM EIF NPM TBQAZ AAHBH AAYXX ALIPV CITATION TDBHL TUROJ 7QL 7T5 7TM 7U7 7U9 C1K F1W H94 H95 K9. L.G M7N |
ID | FETCH-LOGICAL-c451t-de74eb68156494fa8260b37902569f8680c3cb2f836d1f93d7413b7a4c54e7f73 |
ISSN | 1465-3338 0307-9457 |
IngestDate | Fri Aug 16 22:20:23 EDT 2024 Fri Sep 13 03:13:56 EDT 2024 Fri Aug 23 03:59:20 EDT 2024 Thu May 23 23:21:20 EDT 2024 Tue Jun 13 19:49:33 EDT 2023 Wed Dec 27 19:17:08 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c451t-de74eb68156494fa8260b37902569f8680c3cb2f836d1f93d7413b7a4c54e7f73 |
Notes | http://dx.doi.org/10.1080/03079457.2015.1008399 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 25609267 |
PQID | 1661714488 |
PQPubID | 32881 |
PageCount | 5 |
ParticipantIDs | proquest_miscellaneous_1798740449 crossref_primary_10_1080_03079457_2015_1008399 fao_agris_US201500139885 proquest_journals_1661714488 pubmed_primary_25609267 informaworld_taylorfrancis_310_1080_03079457_2015_1008399 |
PublicationCentury | 2000 |
PublicationDate | 2015-03-04 |
PublicationDateYYYYMMDD | 2015-03-04 |
PublicationDate_xml | – month: 03 year: 2015 text: 2015-03-04 day: 04 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: Houghton |
PublicationTitle | Avian pathology |
PublicationTitleAlternate | Avian Pathol |
PublicationYear | 2015 |
Publisher | Taylor & Francis Taylor & Francis Ltd |
Publisher_xml | – name: Taylor & Francis – name: Taylor & Francis Ltd |
References | cit0001 cit0012 cit0010 Soderlund-Venermo M. (cit0011) 2001; 82 Reed L.J. (cit0009) 1938; 27 Palya V.J. (cit0007) 2013 cit0008 cit0006 cit0017 cit0018 cit0004 cit0015 cit0005 cit0016 cit0002 cit0013 cit0003 cit0014 |
References_xml | – ident: cit0004 doi: 10.1093/nar/19.5.1156 – ident: cit0015 doi: 10.1080/03079459408419004 – volume: 82 start-page: 919 year: 2001 ident: cit0011 publication-title: Journal of General Virology doi: 10.1099/0022-1317-82-4-919 contributor: fullname: Soderlund-Venermo M. – ident: cit0003 doi: 10.1556/AVet.53.2005.1.8 – ident: cit0006 doi: 10.1006/viro.1996.0367 – ident: cit0002 doi: 10.1080/03079450094270 – ident: cit0014 doi: 10.1016/j.jviromet.2014.02.014 – ident: cit0017 doi: 10.1016/S1534-5807(01)00031-4 – ident: cit0001 doi: 10.1006/viro.1995.1345 – ident: cit0005 doi: 10.1007/BF01309459 – ident: cit0018 doi: 10.1016/j.virol.2003.09.011 – start-page: 444 volume-title: Diseases of Poultry year: 2013 ident: cit0007 contributor: fullname: Palya V.J. – ident: cit0008 doi: 10.1128/JVI.79.17.11035-11044.2005 – ident: cit0012 doi: 10.1080/03079450410001724067 – ident: cit0010 doi: 10.1080/03079450802356979 – volume: 27 start-page: 493 year: 1938 ident: cit0009 publication-title: American Journal of Hygiene contributor: fullname: Reed L.J. – ident: cit0013 doi: 10.1637/7172 – ident: cit0016 doi: 10.1006/viro.1995.1514 |
SSID | ssj0006552 |
Score | 2.1461635 |
Snippet | Muscovy duck parvovirus (MDPV) infection is a highly contagious and fatal disease of Muscovy ducklings. The infectious clone methodology is a valuable tool to... |
SourceID | proquest crossref pubmed informaworld fao |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 124 |
SubjectTerms | Animal diseases Animals Cairina moschata Cloning Cloning, Molecular - methods DNA Primers - genetics duck eggs ducklings Ducks - virology fibroblasts genome Genome, Viral - genetics Muscovy Muscovy duck parvovirus Mutagenesis Mutation Parvovirus Parvovirus - genetics Parvovirus - pathogenicity Plasmids Plasmids - biosynthesis Plasmids - genetics Poultry Real-Time Polymerase Chain Reaction site-directed mutagenesis Transfection vaccine development Virulence Viruses |
Title | Construction of an infectious plasmid clone of Muscovy duck parvovirus by TA cloning and creation of a partially attenuated strain |
URI | https://www.tandfonline.com/doi/abs/10.1080/03079457.2015.1008399 https://www.ncbi.nlm.nih.gov/pubmed/25609267 https://www.proquest.com/docview/1661714488/abstract/ https://search.proquest.com/docview/1798740449 |
Volume | 44 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Jb9NAFB7R9sIFQVkaKGiQuEWObM9i-5hCowgl7QEHhdNo7LEhIjhVs0jhyC_nzeKlUEThYkVeZpx8X9689-YtCL3hQayKvCw8yknowQqhvCxOJFitUvmMKR6adkDTCz6e0fdzNm-9Sia7ZJMN8u-35pX8D6pwDnDVWbL_gGwzKJyAz4AvHAFhON4JY91ts67_ajb0qya4arvWDaLX3xaqny9XlfEMTLfrfLXb99U2_9q_kte71W5xDTeCBpoOzW11xmJXlZT6VpADy-W-r4txVluptdS1aS7R1W2HOy0sdIvjG676T1awpV6jNE9sBIHXpJZdbo0T1ms9tl8WLmnEG3f9EgEzgVm0I750-cmE2vLTg8KKV8qZR4it51LLX0o7PAs7wjSw2dVuXQ5sEvlvIt_FSMJsejIdrMd06Acofkm7xtX7-heXYjSbTER6Pk8P0FEYJQws9qPh2buzUbOAc8aa-pl6zDrxS5dkv22aGyrNQSlXv5S9_bPxYpSY9CF64KwPPLRUeoTuFdUxOv6oQ6JMXjaeulCLx-hHl1x4VWJZ4ZZc2JELG3Lpy45cWJMLt-TC2R6nQ-zIBYPAI45cZlDckAu35MKWXE_QbHSevh17rmGHl1MWbDxVRLTIuClAlNBSgunqZyTSO9k8KWMe-znJs7CMCVdBmRAF6izJIklzRouojMhTdFjBW58gzEvAxlcxJSWhPuhMueQFVyyJmaRZlPXQoP7NxZWtyyKCutytA0lokIQDqYdOABkhP8PaKWYf9CVj_sQx66GkC5fYGF9ZaRvbCPKXYU9rbIWTDGsRgNIbBRTWxh563VwGua0342RVAExCFwqMqE8pDPHMcqL5ItoMSUIePb_D0y_Q_fbfd4oOgRjFS9CTN9krx-ufx_O3Sg |
link.rule.ids | 315,786,790,27957,27958,60241,61030 |
linkProvider | EBSCOhost |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LbxMxELZoOcCFR3k0UMBIXDdk14-1jxWiCtDkQoJ6s7z2GlUNu1GTjZQe-8uZ8e5GpVLFoWfbo7U9nvlmPf6GkE8yVb50oUy4ZFkCHsInhdIWolbrR0J4mcVyQJOpHM_59zNxduMtDKZVYgwdWqKIaKvxcOPP6D4l7jMqpuYix8wsgff84OX1HnkoweHg4WSj6c4aSyF2ZIg4pn_Fc5eYf_zTXrD1LQ7Tu5Fo9EgnT4nr59ImolwMm3UxdFe3aB7vN9ln5EkHWOlxq2HPyYOyOiAHvzCLJj7lpZPudv4Fucbqnz0fLa0DtRXtk72aFV0CUP9z7qlb1FWJzZNm5erNlvrGXdClvdzUm_NL6Fhs6ew4dgO_CkJgSAdto1DsCnZpsdhSJAetGoDLnq5isYuXZH7ydfZlnHQ1HhLHRbpOfJnzspCRs0bzYCHaGRUsx8tPqYOSauSYK7KgmPRp0MwDAmJFbrkTvMxDzl6R_Qq--pBQGTIY5xVngUFUrLWzspReaCUsL_JiQIb9zpplS-Vh0p4htVtkg4tsukUekEPYf2N_g7k185_YFBGzUmJA9E2lMOv4eyW0tVAM-4_Yo16DTGcwViYFnJRDcKvUgHzcNcNRx_sbW5WwTQa55XI-4hxEvG41bzcRRK46k_mbe3zYB_JoPJucmtNv0x9vyWNsitl2_Ijsg_KU7wB-rYv38Xz9BScuH54 |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZokRAXHuXRhQJG4pplk_h5rIBVeewKiS7iZvlZVV2SVbNZaTnyyxk7yQoqVRx6tj2K7fHMN_H4G4TesFw4b4PPCCuLDDyEy4yQGqJW7SaUOlakckCzOTtZkE8_6JBN2PRplTGGDh1RRLLV8XCvXBgy4t5GvZSE8piYReM1Pzh5uYdus1gyK77imMx3xphRuuNCjGOGRzzXifnHPe0FXV-hML0eiCaHNL2PzDCVLg_lYtyuzdj-usLyeKO5PkD3eriKjzv9eohu-eoAHXyPOTTpIS-e9Xfzj9DvWPtzYKPFdcC6wkOqV9vgFcD0n-cO22Vd-dg8axtbb7bYtfYCr_Tlpt6cX0JHs8Wnx6kbeFUQAkN6YJuExq5glZbLLY7UoFULYNnhJpW6eIwW0w-n706yvsJDZgnN15nznHjDEmONJEFDrDMxJY9Xn0wGwcTEltYUQZTM5UGWDvBPabgmlhLPAy-foP0KvvoQYRYKGOcEKUMJMbGUVjPPHJWCamK4GaHxsLFq1RF5qHzgR-0XWcVFVv0ij9AhbL_SZ2Bs1eJbbEp4WQg6QvJvnVDr9HMldJVQVPkfsUeDAqneXDQqB5TEIbQVYoRe75rhoMfbG1152CYVmeU4mRACIp52irebSMStsmD82Q0-7BW68_X9VH35OP_8HN2NLSnVjhyhfdAd_wKw19q8TKfrD5YiHks |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Construction+of+an+infectious+plasmid+clone+of+Muscovy+duck+parvovirus+by+TA+cloning+and+creation+of+a+partially+attenuated+strain&rft.jtitle=Avian+pathology&rft.au=Yen%2C+T-Y&rft.au=Li%2C+K-P&rft.au=Ou%2C+S-C&rft.au=Shien%2C+J-H&rft.date=2015-03-04&rft.issn=0307-9457&rft.eissn=1465-3338&rft.volume=44&rft.issue=2&rft.spage=124&rft.epage=128&rft_id=info:doi/10.1080%2F03079457.2015.1008399&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1465-3338&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1465-3338&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1465-3338&client=summon |